Comparative Investigation of the Chemiluminescent Properties of a Dibrominated Coelenterazine Analog
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemistry and Spectroscopic Properties of Br2-Cla
2.2. Investigation of Chemiluminescence in Aprotic Solvents
2.3. Investigation of Chemiluminescence in Protic Solvents
3. Materials and Methods
3.1. Synthesis of Br2-Cla
3.2. Clz, Clz400a, Clz-e and CypLuc
3.3. Luminometric and Spectroscopy Characterization
4. Conclusions
5. Patents
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Molecules | Emax | Area | thalf | vinitial |
---|---|---|---|---|
Br2-Cla | 100 ± 10% | 100 ± 10% | 14 ± 1 s | 100 ± 11% |
Clz | 33 ± 6% | 14 ± 2% | 19.2 ± 2.2 s | 55 ± 11% |
Clz400a | 61 ± 2% | 103 ± 5% | 24.8 ± 0.5 s | 66 ± 4% |
CypLuc | 40 ± 3% | 15 ± 1% | 5.9 ± 0.1 s | 55 ± 6% |
Clz-e | 696 ± 45% | 272 ± 17% | 6.1 ± 0.3 s | 702 ± 84% |
Molecules | Emax | Area | thalf | vinitial |
---|---|---|---|---|
Br2-Cla | 100 ± 4% | 100 ± 8% | 79.8 ± 10.2 s | 100 ± 10% |
Clz | 54 ± 8% | 81 ± 11% | 164.0 ± 14.4 s | 51 ± 11% |
Clz400a | 69 ± 8% | 64 ± 14% | 73.7 ± 17.6 s | 90 ± 13% |
CypLuc | 413 ± 37% | 115 ± 5% | 9.5 ± 1.2 s | 317 ± 51% |
Clz-e | 587 ± 71% | 156 ± 17% | 8.2 ± 1.3 s | 676 ± 126% |
Solvent | Emax | Area | thalf | vinitial |
---|---|---|---|---|
DMSO—sodium acetate buffer pH 5.2 (1%) | 100 ± 10% | 100 ± 4% | 40.0 ± 4.8 s | 100 ± 24% |
DMSO—NaOH (0.1 M) | 222 ± 11% | 88 ± 4% | 0.48 ± 0.02 s | 1517 ± 30% |
DMF—sodium acetate buffer pH 5.2 (1%) | 100 ± 4% | 100 ± 5% | 108.5 ± 5.4 s | 100 ± 19% |
DMF—NaOH (0.1 M) | 1253 ± 105% | 96 ± 4% | 0.48 ± 0.05 s | 1999 ± 252% |
Molecule—KO2 Amount | Emax |
---|---|
Br2-Cla—5 mg | 109 ± 9% |
Br2-Cla—10 mg | 100 ± 8% |
Br2-Cla—15 mg | 56 ± 7% |
Clz—5 mg | 3 ± 1% |
Clz—10 mg | 6 ± 1% |
Clz—15 mg | 8 ± 1% |
Clz-e—10 mg | 35 ± 7% |
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Sousa, J.; Magalhães, C.M.; González-Berdullas, P.; Esteves da Silva, J.C.G.; Pinto da Silva, L. Comparative Investigation of the Chemiluminescent Properties of a Dibrominated Coelenterazine Analog. Int. J. Mol. Sci. 2022, 23, 8490. https://doi.org/10.3390/ijms23158490
Sousa J, Magalhães CM, González-Berdullas P, Esteves da Silva JCG, Pinto da Silva L. Comparative Investigation of the Chemiluminescent Properties of a Dibrominated Coelenterazine Analog. International Journal of Molecular Sciences. 2022; 23(15):8490. https://doi.org/10.3390/ijms23158490
Chicago/Turabian StyleSousa, João, Carla M. Magalhães, Patricia González-Berdullas, Joaquim C. G. Esteves da Silva, and Luís Pinto da Silva. 2022. "Comparative Investigation of the Chemiluminescent Properties of a Dibrominated Coelenterazine Analog" International Journal of Molecular Sciences 23, no. 15: 8490. https://doi.org/10.3390/ijms23158490