GmIAA27 Encodes an AUX/IAA Protein Involved in Dwarfing and Multi-Branching in Soybean

Su, Bohong; Wu, Haitao; Guo, Yong; Gao, Huawei; Wei, Zhongyan; Zhao, Yuyang; Qiu, Lijuan

doi:10.3390/ijms23158643

Open AccessArticle

GmIAA27 Encodes an AUX/IAA Protein Involved in Dwarfing and Multi-Branching in Soybean

by

Bohong Su

^1,2,†,

Haitao Wu

^1,†,

Yong Guo

²,

Huawei Gao

²,

Zhongyan Wei

³,

Yuyang Zhao

² and

Lijuan Qiu

^1,2,*

¹

College of Agriculture, Northeast Agricultural University, Harbin 150030, China

²

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

³

Institute of Plant Virology, Ningbo University, Ningbo 315211, China

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2022, 23(15), 8643; https://doi.org/10.3390/ijms23158643

Submission received: 28 June 2022 / Revised: 25 July 2022 / Accepted: 1 August 2022 / Published: 3 August 2022

(This article belongs to the Special Issue Soybean Molecular Breeding and Genetics)

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Abstract

Soybean plant height and branching affect plant architecture and yield potential in soybean. In this study, the mutant dmbn was obtained by treating the cultivar Zhongpin 661 with ethylmethane sulfonate. The dmbn mutant plants were shorter and more branched than the wild type. The genetic analysis showed that the mutant trait was controlled by a semi-dominant gene. The candidate gene was fine-mapped to a 91 kb interval on Chromosome 9 by combining BSA-seq and linkage analysis. Sequence analysis revealed that Glyma.09g193000 encoding an Aux/IAA protein (GmIAA27) was mutated from C to T in the second exon of the coding region, resulting to amino acid substitution of proline to leucine. Overexpression of the mutant type of this gene in Arabidopsis thaliana inhibited apical dominance and promoted lateral branch development. Expression analysis of GmIAA27 and auxin response genes revealed that some GH3 genes were induced. GmIAA27 relies on auxin to interact with TIR1, whereas Gmiaa27 cannot interact with TIR1 owing to the mutation in the degron motif. Identification of this unique gene that controls soybean plant height and branch development provides a basis for investigating the mechanisms regulating soybean plant architecture development.

Keywords: soybean; plant height; branches; AUX/IAA; TIR1

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MDPI and ACS Style

Su, B.; Wu, H.; Guo, Y.; Gao, H.; Wei, Z.; Zhao, Y.; Qiu, L. GmIAA27 Encodes an AUX/IAA Protein Involved in Dwarfing and Multi-Branching in Soybean. Int. J. Mol. Sci. 2022, 23, 8643. https://doi.org/10.3390/ijms23158643

AMA Style

Su B, Wu H, Guo Y, Gao H, Wei Z, Zhao Y, Qiu L. GmIAA27 Encodes an AUX/IAA Protein Involved in Dwarfing and Multi-Branching in Soybean. International Journal of Molecular Sciences. 2022; 23(15):8643. https://doi.org/10.3390/ijms23158643

Chicago/Turabian Style

Su, Bohong, Haitao Wu, Yong Guo, Huawei Gao, Zhongyan Wei, Yuyang Zhao, and Lijuan Qiu. 2022. "GmIAA27 Encodes an AUX/IAA Protein Involved in Dwarfing and Multi-Branching in Soybean" International Journal of Molecular Sciences 23, no. 15: 8643. https://doi.org/10.3390/ijms23158643

APA Style

Su, B., Wu, H., Guo, Y., Gao, H., Wei, Z., Zhao, Y., & Qiu, L. (2022). GmIAA27 Encodes an AUX/IAA Protein Involved in Dwarfing and Multi-Branching in Soybean. International Journal of Molecular Sciences, 23(15), 8643. https://doi.org/10.3390/ijms23158643

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GmIAA27 Encodes an AUX/IAA Protein Involved in Dwarfing and Multi-Branching in Soybean

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