The Role of CD28 and CD8+ T Cells in Keloid Development
Abstract
:1. Introduction
2. Result
2.1. DEGs between Groups N and C
2.2. Identification of Hub Genes
2.3. Functional Annotation of DEGs
2.4. CIBERSORT Immune Cell Analysis and CTD Analysis of Hub Genes CD28 and CD8
2.5. Abnormal Expression of Costimulatory Signaling Molecules
2.6. CD28 Expressed on the Surface of CD8+ T Cells
2.7. CD8+ T Cells and Keloid-Associated Fibroblast Coculture
2.8. Neural Network Model of Keloids
3. Discussion
4. Methods
4.1. Patients
4.2. Fibroblasts from Patients with Keloids Cocultured with CD8+ T Cells
4.3. Neural Network Model
4.4. Exploration of Rabbit Ear Model Construction
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patients | Age of Onset (Years) | Gender | mVSS a |
---|---|---|---|
C6 | 27 | male | 0 |
C10 | 54 | female | 0 |
C15 | 31 | female | 0 |
C16 | 25 | male | 0 |
C19 | 32 | female | 0 |
N7 | 24 | female | 11 |
N8 | 32 | male | 10 |
N9 | 37 | female | 10 |
N14 | 21 | male | 9 |
N24 | 26 | female | 11 |
N25 | 24 | female | 9 |
N36 | 38 | male | 12 |
Gene Set Name | SIZE | ES | NES | Rank at Max |
---|---|---|---|---|
Upregulated | ||||
KEGG_EPITHELIAL_CELL_SIGNALING_IN_HELICOBACTER_PYLORI_INFECTION | 61 | 0.563 | 1.525 | 3156 |
KEGG_BASE_EXCISION_REPAIR | 31 | 0.459 | 1.522 | 6082 |
KEGG_ANTIGEN_PROCESSING_AND_PRESENTATION | 57 | 0.653 | 1.519 | 2554 |
KEGG_T_CELL_RECEPTOR_SIGNALING_PATHWAY | 101 | 0.605 | 1.510 | 3648 |
KEGG_TOLL_LIKE_RECEPTOR_SIGNALING_PATHWAY | 88 | 0.702 | 1.508 | 2235 |
KEGG_PATHOGENIC_ESCHERICHIA_COLI_INFECTION | 45 | 0.499 | 1.505 | 4454 |
Downregulated | ||||
KEGG_DILATED_CARDIOMYOPATHY | 86 | −0.524 | −1.434 | 1865 |
KEGG_HYPERTROPHIC_CARDIOMYOPATHY_HCM | 80 | −0.499 | −1.407 | 2006 |
KEGG_LONG_TERM_POTENTIATION | 65 | −0.390 | −1.300 | 1772 |
KEGG_ADHERENS_JUNCTION | 72 | −0.425 | −1.272 | 2780 |
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Shan, M.; Liu, H.; Hao, Y.; Song, K.; Feng, C.; Wang, Y. The Role of CD28 and CD8+ T Cells in Keloid Development. Int. J. Mol. Sci. 2022, 23, 8862. https://doi.org/10.3390/ijms23168862
Shan M, Liu H, Hao Y, Song K, Feng C, Wang Y. The Role of CD28 and CD8+ T Cells in Keloid Development. International Journal of Molecular Sciences. 2022; 23(16):8862. https://doi.org/10.3390/ijms23168862
Chicago/Turabian StyleShan, Mengjie, Hao Liu, Yan Hao, Kexin Song, Cheng Feng, and Youbin Wang. 2022. "The Role of CD28 and CD8+ T Cells in Keloid Development" International Journal of Molecular Sciences 23, no. 16: 8862. https://doi.org/10.3390/ijms23168862