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Article

Functional Impairment of Endothelial Colony Forming Cells (ECFC) in Patients with Severe Atherosclerotic Cardiovascular Disease (ASCVD)

by
Stéphanie Simoncini
1,†,
Simon Toupance
2,†,
Carlos Labat
2,
Sylvie Gautier
3,
Chloé Dumoulin
4,
Laurent Arnaud
5,
Maria G. Stathopoulou
6,
Sophie Visvikis-Siest
7,
Pascal M. Rossi
1,8,
Athanase Benetos
2,3,
Françoise Dignat-George
1,5 and
Florence Sabatier
1,4,*
1
Aix Marseille Univ, INSERM, INRAE, C2VN, 13005 Marseille, France
2
Université de Lorraine, INSERM, DCAC, 54000 Nancy, France
3
Université de Lorraine, CHRU-Nancy, Department of Geriatrics, 54000 Nancy, France
4
Cell Therapy Laboratory, Hôpital de la Conception, AP-HM, INSERM CIC BT 1409, 13005 Marseille, France
5
Hematology and Vascular Biology Department, Hopital de la Timone, AP-HM, 13005 Marseille, France
6
INSERM, C3M, Team Control of Gene Expression (10), Université Cote d’Azur, 06000 Nice, France
7
Université de Lorraine, IGE-PCV, 54000 Nancy, France
8
Service de Médecine Interne, Hôpital Nord, APHM, 13015 Marseille, France
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2022, 23(16), 8969; https://doi.org/10.3390/ijms23168969
Submission received: 15 July 2022 / Revised: 2 August 2022 / Accepted: 9 August 2022 / Published: 11 August 2022
(This article belongs to the Special Issue Endothelial Progenitor Cells in Health and Disease 2.0)
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Abstract

Endothelial dysfunction is a key factor in atherosclerosis. However, the link between endothelial repair and severity of atherosclerotic cardiovascular disease (ASCVD) is unclear. This study investigates the relationship between ASCVD, markers of inflammation, and circulating endothelial progenitor cells, namely hematopoietic cells with paracrine angiogenic activity and endothelial colony forming cells (ECFC). Two hundred and forty-three subjects from the TELARTA study were classified according to the presence of clinical atherosclerotic disease. ASCVD severity was assessed by the number of involved vascular territories. Flow cytometry was used to numerate circulating progenitor cells (PC) expressing CD34 and those co-expressing CD45, CD34, and KDR. Peripheral blood mononuclear cells ex vivo culture methods were used to determine ECFC and Colony Forming Unit- endothelial cells (CFU-EC). The ECFC subpopulation was analyzed for proliferation, senescence, and vasculogenic properties. Plasma levels of IL-6 and VEGF-A were measured using Cytokine Array. Despite an increased number of circulating precursors in ASCVD patients, ASCVD impaired the colony forming capacity and the angiogenic properties of ECFC in a severity-dependent manner. Alteration of ECFC was associated with increased senescent phenotype and IL-6 levels. Our study demonstrates a decrease in ECFC repair capacity according to ASCVD severity in an inflammatory and senescence-associated secretory phenotype context.
Keywords: endothelial progenitor cells; atherosclerosis; senescence endothelial progenitor cells; atherosclerosis; senescence
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MDPI and ACS Style

Simoncini, S.; Toupance, S.; Labat, C.; Gautier, S.; Dumoulin, C.; Arnaud, L.; Stathopoulou, M.G.; Visvikis-Siest, S.; Rossi, P.M.; Benetos, A.; et al. Functional Impairment of Endothelial Colony Forming Cells (ECFC) in Patients with Severe Atherosclerotic Cardiovascular Disease (ASCVD). Int. J. Mol. Sci. 2022, 23, 8969. https://doi.org/10.3390/ijms23168969

AMA Style

Simoncini S, Toupance S, Labat C, Gautier S, Dumoulin C, Arnaud L, Stathopoulou MG, Visvikis-Siest S, Rossi PM, Benetos A, et al. Functional Impairment of Endothelial Colony Forming Cells (ECFC) in Patients with Severe Atherosclerotic Cardiovascular Disease (ASCVD). International Journal of Molecular Sciences. 2022; 23(16):8969. https://doi.org/10.3390/ijms23168969

Chicago/Turabian Style

Simoncini, Stéphanie, Simon Toupance, Carlos Labat, Sylvie Gautier, Chloé Dumoulin, Laurent Arnaud, Maria G. Stathopoulou, Sophie Visvikis-Siest, Pascal M. Rossi, Athanase Benetos, and et al. 2022. "Functional Impairment of Endothelial Colony Forming Cells (ECFC) in Patients with Severe Atherosclerotic Cardiovascular Disease (ASCVD)" International Journal of Molecular Sciences 23, no. 16: 8969. https://doi.org/10.3390/ijms23168969

APA Style

Simoncini, S., Toupance, S., Labat, C., Gautier, S., Dumoulin, C., Arnaud, L., Stathopoulou, M. G., Visvikis-Siest, S., Rossi, P. M., Benetos, A., Dignat-George, F., & Sabatier, F. (2022). Functional Impairment of Endothelial Colony Forming Cells (ECFC) in Patients with Severe Atherosclerotic Cardiovascular Disease (ASCVD). International Journal of Molecular Sciences, 23(16), 8969. https://doi.org/10.3390/ijms23168969

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