The p53 and Calcium Regulated Actin Rearrangement in Model Cells
Abstract
:1. Introduction
2. Results
2.1. p53 Forms Dimers and Binds to Actin Monomers and Filaments
2.2. JMY Reorders Actin Filaments by Millimolar Calcium
2.3. Actin Competes with p53 on JMY and p53 Competes with Actin on GSN in a Calcium-Dependent Manner
2.4. Increased Cytoplasmic GSN Level in HeLa Cells
2.5. High Cytoplasmic GSN or JMY Affects the Division and Motility of HeLa Cells
3. Discussion
4. Materials and Methods
4.1. Proteins
4.2. Fluorescence Spectroscopy
4.3. Cosedimentation
4.4. Low-Voltage Electroporation
4.5. Cell Culture
4.6. Cell Fixation, Labeling
4.7. Microscopy, Confocal Microscopy
4.8. Scratching Method
4.9. Image Analysis
4.10. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hencz, A.; Szabó-Meleg, E.; Dayo, M.Y.; Bilibani, A.; Barkó, S.; Nyitrai, M.; Szatmári, D. The p53 and Calcium Regulated Actin Rearrangement in Model Cells. Int. J. Mol. Sci. 2022, 23, 9078. https://doi.org/10.3390/ijms23169078
Hencz A, Szabó-Meleg E, Dayo MY, Bilibani A, Barkó S, Nyitrai M, Szatmári D. The p53 and Calcium Regulated Actin Rearrangement in Model Cells. International Journal of Molecular Sciences. 2022; 23(16):9078. https://doi.org/10.3390/ijms23169078
Chicago/Turabian StyleHencz, Alexandra, Edina Szabó-Meleg, Muhammad Yaqoob Dayo, Ardora Bilibani, Szilvia Barkó, Miklós Nyitrai, and Dávid Szatmári. 2022. "The p53 and Calcium Regulated Actin Rearrangement in Model Cells" International Journal of Molecular Sciences 23, no. 16: 9078. https://doi.org/10.3390/ijms23169078