Profiling of the Candidate Interacting Proteins of SELF-PRUNING 6A (SP6A) in Solanum tuberosum
Abstract
:1. Introduction
2. Results
2.1. Yeast Two-Hybrid Library Construction
2.2. Screening of StSP6A Interactors
2.3. Yeast Two-Hybrid and Bimolecular Fluorescence Complementation Analysis of the Interactors of StSP6A In Vitro and In Vivo
2.4. Subcellular Localization of the StFPF1.1, StNFL1, and StNFL2
2.5. Expression Patterns of StFPF1.1, StNFL1, StNFL2, and StSP6A in Response to Tuberization or Flowering
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. RNA Extraction and Quantitative Reverse Transcription PCR Analysis
4.3. Yeast Library Construction
4.4. BK-StSP6A Vector Construction
4.5. Y2H Library Screening and Y2H Assays
4.6. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Enrichment Analysis and Visualization
4.7. Bimolecular Fluorescence Complementation (BiFC) Assays
4.8. Subcellular Localization
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, E.; Liu, T.; Sun, X.; Jing, S.; Zhou, T.; Liu, T.; Song, B. Profiling of the Candidate Interacting Proteins of SELF-PRUNING 6A (SP6A) in Solanum tuberosum. Int. J. Mol. Sci. 2022, 23, 9126. https://doi.org/10.3390/ijms23169126
Wang E, Liu T, Sun X, Jing S, Zhou T, Liu T, Song B. Profiling of the Candidate Interacting Proteins of SELF-PRUNING 6A (SP6A) in Solanum tuberosum. International Journal of Molecular Sciences. 2022; 23(16):9126. https://doi.org/10.3390/ijms23169126
Chicago/Turabian StyleWang, Enshuang, Tengfei Liu, Xiaomeng Sun, Shenglin Jing, Tingting Zhou, Tiantian Liu, and Botao Song. 2022. "Profiling of the Candidate Interacting Proteins of SELF-PRUNING 6A (SP6A) in Solanum tuberosum" International Journal of Molecular Sciences 23, no. 16: 9126. https://doi.org/10.3390/ijms23169126