Effect of Protein–Protein Interactions on Translational Diffusion of Spheroidal Proteins

Round 1

Reviewer 1 Report

The manuscript is written very well and also the conclusions are consistent with the data but both in the introduction and in the conclusion section, there is no explicit explanation of how this study can help to understand the biological processes in which PPIs are involved and in particular how translational diffusion can be affected or in turn affect PPIs above all in diseases where often PPIs are dysregulated.

Author Response

Dear Reviewer! Thank you for Your comments on our manuscript. The PPIs are involved in all protein functioning, which are difficult to enumerate. The knowledge on PPI is necessary and useful in different fields of protein science. The translational diffusion, as well is many other dynamic processes, reflects many aspects of PPI. Here we have shown and analyzed comparatively simple and direct experimental approach to estimate PPI, which can be applied to any specific protein in different conditions.

We added the following information in Conclusion section of our manuscript:

PPIs have the pivotal role in biological processes in living systems, controlling and modulating the direction protein functioning, such as, for example, signal transduction, associated with various diseases, including cancer, infections and neurodegenerative diseases [138].

Reviewer 2 Report

The review summarized the PPI using the self and collective diffusion constants using different models of proteins. The review is well written and clearly organized in different subjects including varied  protein and salt concentrations. The interaction potentionals of proteins and assumptions of spherical genometry are generally consistent with the experimental data of PPI. I recommend this review to be published as is.

Author Response

Dear Reviewer! Thank You for Your appreciation of our article.