Genetic Variation in the MBL2 Gene Is Associated with Chlamydia trachomatis Infection and Host Humoral Response to Chlamydia trachomatis Infection
Abstract
:1. Introduction
2. Results
2.1. Study Sample Characteristics
2.2. SNP Distribution
2.3. Association of SNPs and Stadium of Infection
2.4. Effect of Homozygous and Heterozygous Carriage of Alleles and Susceptibility to Infection
2.5. Haplotype Frequencies and Associations with Susceptibility
2.6. Association of SNPs with Immune Response
3. Discussion
4. Materials and Methods
4.1. Sample Collection
4.2. Ethical Approval
4.3. Laboratory Tests
4.4. Genotype Analysis
4.5. Subgroups for Analyses
4.6. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
SNP | single-nucleotide polymorphism |
MBL | mannose-binding lectin |
IgG | immunoglobulin G |
ELISA | enzyme-linked immunosorbent assay |
PCR | polymerase chain reaction |
OR | odds ratio |
CI | confidence interval |
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Chlamydia trachomatis Infection | ||||||
---|---|---|---|---|---|---|
Overall SNP Distribution | Ct-DNA+/IgG+ | Ct-DNA+/IgG− | Ct-DNA−/IgG+ | Ct-DNA−/IgG− | ||
n = 492 (%) | n = 139 (%) | n = 65 (%) | n = 73 (%) | n = 215 (%) | ||
−619 G > C | GG | 203 (41) | 59 (42) | 28 (43) | 39 (53) * | 77 (36) |
GC | 224 (46) | 67 (48) | 30 (46) | 24 (33) | 103 (48) | |
CC | 65 (13) | 13 (9.4) | 7 (11) | 10 (14) | 35 (16) | |
−290 G > C | GG | 301 (61) | 88 (63) | 35 (54) | 41 (56) | 137 (64) |
GC | 167 (34) | 45 (32) | 29 (45) | 27 (37) | 66 (31) | |
CC | 24 (4.9) | 6 (4.3) | 1 (1.5) | 5 (6.8) | 12 (5.6) | |
−66 C > T | CC | 280 (57) | 76 (55) | 36 (55) | 41 (56) | 127 (59) |
CT | 187 (38) | 51 (37) | 27 (42) | 28 (38) | 81 (38) | |
TT | 25 (5.1) | 12 (8.6) | 2 (3.1) | 4 (5.5) | 7 (3.3) | |
+154 C > T | CC | 430 (87) | 122 (88) | 58 (89) | 63 (86) | 187 (87) |
CT | 60 (12) | 15 (11) | 7 (11) | 10 (14) | 28 (13) | |
TT | 2 (0.4) | 2 (1.4) | 0 (0.0) | 0 (0.0) | 0 (0.0) | |
+161 A > G | AA | 386 (78) | 108 (78) | 49 (75) | 57 (78) | 172 (80) |
AG | 99 (20) | 28 (20) | 15 (23) | 15 (21) | 41 (19) | |
GG | 7 (1.4) | 3 (2.2) | 1 (1.5) | 1 (1.4) | 2 (0.9) | |
+170 A > G | AA | 459 (93) | 123 (89) | 63 (97) | 69 (95) | 204 (95) |
AG | 32 (7) | 15 (11) | 2 (3.1) | 4 (5.5) | 11 (5.1) | |
GG | 1 (0.2) | 1 (0.7) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
Ct-DNA+/IgG+ | Ct-DNA+/IgG− | Ct-DNA−/IgG+ | Ct-DNA−/IgG− | ||
---|---|---|---|---|---|
MBL Production | n = 139 (%) | n = 65 (%) | n = 73 (%) | n = 215 (%) | |
HYA/HYA | High | 7 (5.0) * | 4 (6.2) | 6 (8.2) | 27 (13) |
HYA/LYA | 22 (16) | 12 (19) | 8 (11) | 37 (17) | |
HYA/LXA | 19 (14) | 8 (12) | 5 (6.8) | 26 (12) | |
LYA/LYA | 10 (7.2) | 2 (3.1) | 8 (11) | 13 (6.0) | |
LYA/LXA | 14 (10) | 12 (19) | 13 (18) | 23 (11) | |
LXA/LXA | Low | 6 (4.3) | 1 (1.5) | 5 (6.8) | 12 (5.6) |
HYA/O | 19 (14) | 9 (14) | 9 (12) | 28 (13) | |
LYA/O | 21 (15) | 7 (11) | 7 (9.6) | 25 (12) | |
LXA/O | Deficient | 12 (8.6) | 9 (14) | 9 (12) | 17 (7.9) |
O/O | 9 (6.5) | 1 (1.5) | 3 (4.1) | 7 (3.3) |
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Verweij, S.P.; Peters, R.P.H.; Catsburg, A.; de Vries, H.J.C.; Ouburg, S.; Morré, S.A. Genetic Variation in the MBL2 Gene Is Associated with Chlamydia trachomatis Infection and Host Humoral Response to Chlamydia trachomatis Infection. Int. J. Mol. Sci. 2022, 23, 9292. https://doi.org/10.3390/ijms23169292
Verweij SP, Peters RPH, Catsburg A, de Vries HJC, Ouburg S, Morré SA. Genetic Variation in the MBL2 Gene Is Associated with Chlamydia trachomatis Infection and Host Humoral Response to Chlamydia trachomatis Infection. International Journal of Molecular Sciences. 2022; 23(16):9292. https://doi.org/10.3390/ijms23169292
Chicago/Turabian StyleVerweij, Stephan P., Remco P. H. Peters, Arnold Catsburg, Henry J. C. de Vries, Sander Ouburg, and Servaas A. Morré. 2022. "Genetic Variation in the MBL2 Gene Is Associated with Chlamydia trachomatis Infection and Host Humoral Response to Chlamydia trachomatis Infection" International Journal of Molecular Sciences 23, no. 16: 9292. https://doi.org/10.3390/ijms23169292