Salicylic Acid Regulates Root Gravitropic Growth via Clathrin-Independent Endocytic Trafficking of PIN2 Auxin Transporter in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. High-Concentration SA Application Affects Root Gravitropic Growth in a Dose-Dependent Manner
2.2. SA Reduces Auxin Accumulation and Distribution in the Root Apical Meristem
2.3. SA Reduces PIN2 Accumulation and Distribution in the Roots
2.4. SA-Induced PIN2 Internalization Is Independent of Clathrin-Mediated Endocytosis
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Growth Condition
4.3. Root Gravitropism Assay
4.4. Confocal Microscopy Analysis
4.5. Quantitative Real-Time PCR (qRT-PCR)
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhou, H.; Ge, H.; Chen, J.; Li, X.; Yang, L.; Zhang, H.; Wang, Y. Salicylic Acid Regulates Root Gravitropic Growth via Clathrin-Independent Endocytic Trafficking of PIN2 Auxin Transporter in Arabidopsis thaliana. Int. J. Mol. Sci. 2022, 23, 9379. https://doi.org/10.3390/ijms23169379
Zhou H, Ge H, Chen J, Li X, Yang L, Zhang H, Wang Y. Salicylic Acid Regulates Root Gravitropic Growth via Clathrin-Independent Endocytic Trafficking of PIN2 Auxin Transporter in Arabidopsis thaliana. International Journal of Molecular Sciences. 2022; 23(16):9379. https://doi.org/10.3390/ijms23169379
Chicago/Turabian StyleZhou, Houjun, Haiman Ge, Jiahong Chen, Xueqin Li, Lei Yang, Hongxia Zhang, and Yuan Wang. 2022. "Salicylic Acid Regulates Root Gravitropic Growth via Clathrin-Independent Endocytic Trafficking of PIN2 Auxin Transporter in Arabidopsis thaliana" International Journal of Molecular Sciences 23, no. 16: 9379. https://doi.org/10.3390/ijms23169379