In Vitro and In Vivo Activities of Tilmicosin and Acetylisovaleryltylosin Tartrate against Toxoplasma gondii
Abstract
:1. Introduction
2. Results
2.1. Cytotoxicity Activity of Tilmicosin and ATLL
2.2. Tilmicosin and ATLL Have Good Anti-T. gondii Effect In Vitro
2.3. Differential Effects of Tilmicosin and ATLL on Tachyzoite Invasion and Proliferation
2.4. Effect of ATLL on the Morphology and Division of Tachyzoites
2.5. Tilmicosin and ATLL Do Not Affect the Mitochondria and Apicoplast of Tachyzoites
2.6. Tilmicosin and ATLL Delay Mortality in Mice Caused by Acute Toxoplasmosis
3. Discussion
4. Materials and Methods
4.1. Ethical Statement
4.2. Parasites and Cell Culture
4.3. Compounds
4.4. Cytotoxicity Assay
4.5. Plaque Assay
4.6. Immunofluorescence Assays
4.7. Invasion Assay and Intracellular Replication Assay
4.8. In Vitro Drug Inhibition Assay for T. gondii
4.9. T. gondii Intracellular and Extracellular Inhibition Assay
4.10. Parasite Division and Morphological Observation by Immunofluorescence Assays
4.11. Therapeutic Effect of Tilmicosin and ATLL on Acute Toxoplasmosis in Mice
4.12. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Ma, Y.; Cao, X.; Wang, H.; Song, X.; Hu, D. In Vitro and In Vivo Activities of Tilmicosin and Acetylisovaleryltylosin Tartrate against Toxoplasma gondii. Int. J. Mol. Sci. 2022, 23, 9586. https://doi.org/10.3390/ijms23179586
Ma Y, Cao X, Wang H, Song X, Hu D. In Vitro and In Vivo Activities of Tilmicosin and Acetylisovaleryltylosin Tartrate against Toxoplasma gondii. International Journal of Molecular Sciences. 2022; 23(17):9586. https://doi.org/10.3390/ijms23179586
Chicago/Turabian StyleMa, Yazhen, Xinru Cao, Hui Wang, Xingju Song, and Dandan Hu. 2022. "In Vitro and In Vivo Activities of Tilmicosin and Acetylisovaleryltylosin Tartrate against Toxoplasma gondii" International Journal of Molecular Sciences 23, no. 17: 9586. https://doi.org/10.3390/ijms23179586