Photosynthetic Characteristics and Chloroplast Ultrastructure Responses of Citrus Leaves to Copper Toxicity Induced by Bordeaux Mixture in Greenhouse
Abstract
:1. Introduction
2. Results
2.1. Effects of Excessive Spraying of Bm on the Leaf Morphology and Cu Content of Two Citrus Species
2.2. Effects of Excessive Spraying of Bm on the Leaf Photosynthetic Pigments of Two Citrus Species
2.3. Effects of Excessive Spraying of Bm on the Leaf Gas Exchange of Two Citrus Species
2.4. Effects of Excessive Spraying of Bm on the Leaf Chl a Fluorescence (OJIP) Transients of Two Citrus Species
2.5. Effects of Excessive Spraying of Bm on the Ultrastructure of Leaf Chloroplast of Two Citrus Species
2.6. Effects of Excessive Spraying of Bm on the Leaf ELR and Activities of SOD, POD and CAT of Two Citrus Species
3. Discussion
4. Materials and Methods
4.1. Seedlings Culture and Treatments
4.2. Cu Quantification of Citrus Leaves
4.3. Extraction and Quantification of Photosynthetic Pigments of Citrus Leaves
4.4. Gas Exchange Measurement of Citrus Leaves
4.5. Chl a Fluorescence (OJIP) Transients Analyses of Citrus Leaves
4.6. Transmission Electron Microscopy (TEM) Analyses of Citrus Leaves
4.7. The ELR and Activities of SOD, POD and CAT of Citrus Leaves
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lu, F.; Hu, P.; Lin, M.; Ye, X.; Chen, L.; Huang, Z. Photosynthetic Characteristics and Chloroplast Ultrastructure Responses of Citrus Leaves to Copper Toxicity Induced by Bordeaux Mixture in Greenhouse. Int. J. Mol. Sci. 2022, 23, 9835. https://doi.org/10.3390/ijms23179835
Lu F, Hu P, Lin M, Ye X, Chen L, Huang Z. Photosynthetic Characteristics and Chloroplast Ultrastructure Responses of Citrus Leaves to Copper Toxicity Induced by Bordeaux Mixture in Greenhouse. International Journal of Molecular Sciences. 2022; 23(17):9835. https://doi.org/10.3390/ijms23179835
Chicago/Turabian StyleLu, Fei, Pingping Hu, Meilan Lin, Xin Ye, Lisong Chen, and Zengrong Huang. 2022. "Photosynthetic Characteristics and Chloroplast Ultrastructure Responses of Citrus Leaves to Copper Toxicity Induced by Bordeaux Mixture in Greenhouse" International Journal of Molecular Sciences 23, no. 17: 9835. https://doi.org/10.3390/ijms23179835