Ionomic Combined with Transcriptomic and Metabolomic Analyses to Explore the Mechanism Underlying the Effect of Melatonin in Relieving Nutrient Stress in Apple
Abstract
:1. Introduction
2. Results
2.1. Exogenous Melatonin Regulates the Growth and Development of Apple Plants and Activates the Antioxidant System under Nutrient Stress
2.2. Exogenous Melatonin Mediates the Regulation of Stomatal Configuration under Nutrient Stress
2.3. Exogenous Melatonin Mediates the Regulation of the Concentration, Uptake, Transfer, and Distribution of Minerals in Plants under Nutrient Stress
2.4. Differentially Expressed Gene Analysis
2.5. Validation of DEGs by qRT-PCR
2.6. Metabolic Response of Apple Trees to Nutrient Stress
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Experimental Design
4.3. Measurement of Growth Characteristics and Root Architecture
4.4. Determination of TCC, TTC, REL, MDA, and Antioxidant Enzyme Activity
4.5. Determination of the Stomatal Configuration
4.6. Determination of Minerals
4.7. Extracting RNA and Transcriptome Sequencing
4.8. qRT-PCR Validation
4.9. Metabolite Analysis
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cao, Y.; Du, P.; Ji, J.; He, X.; Zhang, J.; Shang, Y.; Liu, H.; Xu, J.; Liang, B. Ionomic Combined with Transcriptomic and Metabolomic Analyses to Explore the Mechanism Underlying the Effect of Melatonin in Relieving Nutrient Stress in Apple. Int. J. Mol. Sci. 2022, 23, 9855. https://doi.org/10.3390/ijms23179855
Cao Y, Du P, Ji J, He X, Zhang J, Shang Y, Liu H, Xu J, Liang B. Ionomic Combined with Transcriptomic and Metabolomic Analyses to Explore the Mechanism Underlying the Effect of Melatonin in Relieving Nutrient Stress in Apple. International Journal of Molecular Sciences. 2022; 23(17):9855. https://doi.org/10.3390/ijms23179855
Chicago/Turabian StyleCao, Yang, Peihua Du, Jiahao Ji, Xiaolong He, Jiran Zhang, Yuwei Shang, Huaite Liu, Jizhong Xu, and Bowen Liang. 2022. "Ionomic Combined with Transcriptomic and Metabolomic Analyses to Explore the Mechanism Underlying the Effect of Melatonin in Relieving Nutrient Stress in Apple" International Journal of Molecular Sciences 23, no. 17: 9855. https://doi.org/10.3390/ijms23179855