The Nursehound Scyliorhinus stellaris Mitochondrial Genome—Phylogeny, Relationships among Scyliorhinidae and Variability in Waters of the Balearic Islands
Abstract
:1. Introduction
2. Results and Discussion
2.1. Mitochondrial Genome Organization
2.2. Ribosomal and Transfer RNA Genes
2.3. Noncoding Regions
Variability in Balearic Sea
2.4. Protein-Coding Genes
2.5. Multigenes Phylogenies and Genetic Distance
3. Materials and Methods
3.1. Fish Sampling
3.2. DNA Isolation, Amplification and Purification
3.3. Sequence Analysis
3.4. Mitogenomes Comparison, Genetic Distance and Phylogenetic Analyses
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Position | Strand | Length | Codons | Spacer | ||||
---|---|---|---|---|---|---|---|---|
Gene Description | Start | Stop | nt | AA | Start | Stop | nt | |
tRNA-F PHE (TTC) | 1 | 70 | H | 70 | 0 | |||
rRNA12S | 71 | 1028 | H | 958 | 0 | |||
tRNA-V VAL (GTA) | 1029 | 1100 | H | 72 | 0 | |||
rRNA16S | 1101 | 2772 | H | 1672 | 0 | |||
tRNA-L2 LEU (TTA) | 2773 | 2847 | H | 75 | 0 | |||
ND1 | 2848 | 3822 | H | 975 | 324 | ATG | TAA | 0 |
tRNA-I ILE (ATC) | 3826 | 3895 | H | 70 | 3 | |||
tRNA-Q GLN (CAA) | 3897 | 3968 | L | 72 | 1 | |||
tRNA-M MET (ATG) | 3969 | 4038 | H | 70 | 0 | |||
ND2 | 4039 | 5085 | H | 1047 | 348 | ATG | TAA | 0 |
tRNA-W TRP (TGA) | 5085 | 5153 | H | 69 | −1 | |||
tRNA-A ALA (GCA) | 5155 | 5223 | L | 69 | 1 | |||
tRNA-N ASN (AAC) | 5224 | 5296 | L | 73 | 0 | |||
OriL | 5294 | 5344 | - | 51 | −3 | |||
tRNA-C CYS (TGC) | 5334 | 5400 | L | 67 | −11 | |||
tRNA-Y TYR (TAC) | 5402 | 5471 | L | 70 | 1 | |||
COI | 5473 | 7026 | H | 1554 | 517 | GTG | TAA | 1 |
tRNA-S2 SER (TCA) | 7027 | 7097 | L | 71 | 0 | |||
tRNA-D ASP (GAC) | 7098 | 7166 | H | 69 | 0 | |||
COII | 7172 | 7867 | H | 696 | 231 | ATG | AGA | 5 |
tRNA-K LYS (AAA) | 7860 | 7933 | H | 74 | −8 | |||
ATP8 | 7935 | 8102 | H | 168 | 55 | ATG | TAA | 1 |
ATP6 | 8093 | 8776 | H | 684 | 227 | ATG | TAA | −10 |
COIII | 8776 | 9561 | H | 786 | 261 | ATG | TAA | −1 |
tRNA-G GLY (GGA) | 9564 | 9633 | H | 70 | 3 | |||
ND3 | 9634 | 9984 | H | 351 | 116 | ATG | TAA | 0 |
tRNA-R ARG (CGA) | 9983 | 10,052 | H | 70 | −2 | |||
ND4L | 10,053 | 10,349 | H | 297 | 98 | ATG | TAA | 0 |
ND4 | 10,343 | 11,723 | H | 1381 | 460 | ATG | T-- | −7 |
tRNA-H HIS (CAC) | 11,724 | 11,792 | H | 69 | 0 | |||
tRNA-S1 SER (AGC) | 11,793 | 11,859 | H | 67 | 0 | |||
tRNA-L1 LEU (CTA) | 11,860 | 11,931 | H | 72 | 0 | |||
ND5 | 11,932 | 13,761 | H | 1830 | 609 | ATG | TAG | 0 |
ND6 | 13,758 | 14,279 | L | 522 | 173 | ATG | TAA | −4 |
tRNA-E GLU (GAA) | 14,280 | 14,349 | L | 70 | 0 | |||
CYTB | 14,352 | 15,495 | H | 1144 | 381 | ATG | T-- | 2 |
tRNA-T THR (ACA) | 15,496 | 15,567 | H | 72 | 0 | |||
tRNA-P PRO (CCA) | 15,568 | 15,636 | L | 69 | 0 | |||
DLOOP | 15,637 | 16,684 | - | 1048 | 0 |
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Catanese, G.; Morey, G.; Verger, F.; Grau, A.M. The Nursehound Scyliorhinus stellaris Mitochondrial Genome—Phylogeny, Relationships among Scyliorhinidae and Variability in Waters of the Balearic Islands. Int. J. Mol. Sci. 2022, 23, 10355. https://doi.org/10.3390/ijms231810355
Catanese G, Morey G, Verger F, Grau AM. The Nursehound Scyliorhinus stellaris Mitochondrial Genome—Phylogeny, Relationships among Scyliorhinidae and Variability in Waters of the Balearic Islands. International Journal of Molecular Sciences. 2022; 23(18):10355. https://doi.org/10.3390/ijms231810355
Chicago/Turabian StyleCatanese, Gaetano, Gabriel Morey, Francesc Verger, and Antonio Maria Grau. 2022. "The Nursehound Scyliorhinus stellaris Mitochondrial Genome—Phylogeny, Relationships among Scyliorhinidae and Variability in Waters of the Balearic Islands" International Journal of Molecular Sciences 23, no. 18: 10355. https://doi.org/10.3390/ijms231810355