LPS-Induced Coagulation and Neuronal Damage in a Mice Model Is Attenuated by Enoxaparin
Abstract
:1. Introduction
2. Results
2.1. General Health of the LPS Mice
2.2. Motor Functions
2.3. Heat Sensitivity Function
2.4. Open Field Test
2.5. Y-Maze
2.6. Thrombin Activity
2.7. Hippocampal Gene Expression of Coagulation and Inflammatory Factors
2.8. Serum Markers for Axonal Damage and Inflammation
3. Discussion
4. Materials and Methods
4.1. Model Establishment and Treatment Protocol
4.2. Rotarod
4.3. Hot Plate
4.4. Behavioral Tests
4.5. Open Field
4.6. Y-Maze
4.7. Thrombin Activity Assay
4.8. Coagulation and Inflammation Gene Expression in the Hippocampus
4.9. Neurofilament Light Chain and Tumor Necrosis Factor-α Measurement in the Serum
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Forward | Reverse |
---|---|---|
PAR1 | GCCTCCATCATGCTCATGAC | AAAGCAGACGATGAAGATGCA |
PT | CCGAAAGGGCAACCTAGAGC | GGCCCAGAACACGTCTGTG |
FX | GTGGCCGGGAATGCAA | AACCCTTCATTGTCTTCGTTAATGA |
TNF-α | GACCCTCACACTCAGATCATCTTCT | CCTCCACTTGGTGGTTTGCT |
IL1-β | CTGGTGTGTGACGTTCCCATTA | CCGACAGCACGAGGCTTT |
HPRT | GATTAGCGATGATGAACCAGGTT | CCTCCCATCTCCTTCATGA CA |
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Berkowitz, S.; Gofrit, S.G.; Aharoni, S.A.; Golderman, V.; Qassim, L.; Goldberg, Z.; Dori, A.; Maggio, N.; Chapman, J.; Shavit-Stein, E. LPS-Induced Coagulation and Neuronal Damage in a Mice Model Is Attenuated by Enoxaparin. Int. J. Mol. Sci. 2022, 23, 10472. https://doi.org/10.3390/ijms231810472
Berkowitz S, Gofrit SG, Aharoni SA, Golderman V, Qassim L, Goldberg Z, Dori A, Maggio N, Chapman J, Shavit-Stein E. LPS-Induced Coagulation and Neuronal Damage in a Mice Model Is Attenuated by Enoxaparin. International Journal of Molecular Sciences. 2022; 23(18):10472. https://doi.org/10.3390/ijms231810472
Chicago/Turabian StyleBerkowitz, Shani, Shany Guly Gofrit, Shay Anat Aharoni, Valery Golderman, Lamis Qassim, Zehavit Goldberg, Amir Dori, Nicola Maggio, Joab Chapman, and Efrat Shavit-Stein. 2022. "LPS-Induced Coagulation and Neuronal Damage in a Mice Model Is Attenuated by Enoxaparin" International Journal of Molecular Sciences 23, no. 18: 10472. https://doi.org/10.3390/ijms231810472