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Volume 23
Issue 18
10.3390/ijms231810556
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Article

Comparison between Immunocytochemistry, FISH and NGS for ALK and ROS1 Rearrangement Detection in Cytological Samples

by
Diane Frankel
1,
Isabelle Nanni
2,
L’Houcine Ouafik
2,3,
Clara Camilla
2,
Eric Pellegrino
2,
Nathalie Beaufils
2,
Laurent Greillier
4,
Hervé Dutau
5,
Philippe Astoul
5,
Elise Kaspi
1 and
Patrice Roll
1,*
1
Aix Marseille Univ, APHM, INSERM, MMG, Hôpital la Timone, Service de Biologie Cellulaire, 13005 Marseille, France
2
APHM, Faculté de Médecine Nord, Service d’Oncobiologie, 13015 Marseille, France
3
Aix Marseille Univ, CNRS, INP, Inst Neurophysiopathol, 13005 Marseille, France
4
Multidisciplinary Oncology and Therapeutic Innovations, Marseille University Hospital (APHM), Aix Marseille University, 13015 Marseille, France
5
Department of Thoracic Oncology, Pleural Diseases and Interventional Pulmonology, APHM, 13015 Marseille, France
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(18), 10556; https://doi.org/10.3390/ijms231810556
Submission received: 2 August 2022 / Revised: 30 August 2022 / Accepted: 6 September 2022 / Published: 12 September 2022
(This article belongs to the Collection Feature Papers in Molecular Pathology, Diagnostics, and Therapeutics)
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Abstract

The detection of ROS1 and ALK rearrangements is performed for advanced-stage non-small cell lung cancer. Several techniques can be used on cytological samples, such as immunocytochemistry (ICC), fluorescence in situ hybridization (FISH) and, more recently, next-generation sequencing (NGS), which is gradually becoming the gold standard. We performed a retrospective study to compare ALK and ROS1 rearrangement results from immunocytochemistry, FISH and NGS methods from 131 cytological samples. Compared to NGS, the sensitivity and specificity of ICC were 0.79 and 0.91, respectively, for ALK, and 1 and 0.87 for ROS1. Regarding FISH, the sensitivity and specificity were both at 1 for ALK and ROS1 probes. False-positive cases obtained by ICC were systematically corrected by FISH. When using ICC and FISH techniques, results are very close to NGS. The false-positive cases obtained by ICC are corrected by FISH, and the true-positive cases are confirmed. NGS has the potential to improve the detection of ALK and ROS1 rearrangements in cytological samples; however, the cost of this technique is still much higher than the sequential use of ICC and FISH.
Keywords: immunocytochemistry; FISH; NGS; ALK; ROS1; lung cancer; adenocarcinoma; cytology immunocytochemistry; FISH; NGS; ALK; ROS1; lung cancer; adenocarcinoma; cytology

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MDPI and ACS Style

Frankel, D.; Nanni, I.; Ouafik, L.; Camilla, C.; Pellegrino, E.; Beaufils, N.; Greillier, L.; Dutau, H.; Astoul, P.; Kaspi, E.; et al. Comparison between Immunocytochemistry, FISH and NGS for ALK and ROS1 Rearrangement Detection in Cytological Samples. Int. J. Mol. Sci. 2022, 23, 10556. https://doi.org/10.3390/ijms231810556

AMA Style

Frankel D, Nanni I, Ouafik L, Camilla C, Pellegrino E, Beaufils N, Greillier L, Dutau H, Astoul P, Kaspi E, et al. Comparison between Immunocytochemistry, FISH and NGS for ALK and ROS1 Rearrangement Detection in Cytological Samples. International Journal of Molecular Sciences. 2022; 23(18):10556. https://doi.org/10.3390/ijms231810556

Chicago/Turabian Style

Frankel, Diane, Isabelle Nanni, L’Houcine Ouafik, Clara Camilla, Eric Pellegrino, Nathalie Beaufils, Laurent Greillier, Hervé Dutau, Philippe Astoul, Elise Kaspi, and et al. 2022. "Comparison between Immunocytochemistry, FISH and NGS for ALK and ROS1 Rearrangement Detection in Cytological Samples" International Journal of Molecular Sciences 23, no. 18: 10556. https://doi.org/10.3390/ijms231810556

APA Style

Frankel, D., Nanni, I., Ouafik, L., Camilla, C., Pellegrino, E., Beaufils, N., Greillier, L., Dutau, H., Astoul, P., Kaspi, E., & Roll, P. (2022). Comparison between Immunocytochemistry, FISH and NGS for ALK and ROS1 Rearrangement Detection in Cytological Samples. International Journal of Molecular Sciences, 23(18), 10556. https://doi.org/10.3390/ijms231810556

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