Engineering Nanofiber Scaffolds with Biomimetic Cues for Differentiation of Skin-Derived Neural Crest-like Stem Cells to Schwann Cells
Abstract
:1. Introduction
2. Results
2.1. Fiber Formation and Decoration with Biological Cues
2.2. Effect of Biomolecules on Adhesion and Spreading of KC-NC
2.3. Effect of the Biological Cues on Differentiation of KC-NC Cells to SC Phenotype
3. Discussion
4. Materials and Methods
4.1. Materials
4.1.1. Development of Anisotropically Aligned PCL-PDA
4.1.2. Synthesis of Hep-Thiol
4.1.3. Surface Functionalization with Hep-Thiol and Conjugation of the Biological Cues
4.1.4. Fiber Characterization Measurements
4.1.5. Cloning and Production of the Recombinant Fusion Proteins
4.1.6. Release Kinetics of the Immobilized NRG1 from PCL Fiber Surface
4.2. Cell Culture
4.2.1. Epidermal Cell Isolation
4.2.2. Induction of KC into KC-NC
4.2.3. Proliferation and Differentiation of KC-NC
4.2.4. Immunocytochemistry
4.2.5. Fluorescence Microscopy and Image Analysis
4.2.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
BSA | Bovine serum albumin |
BDNF | Brain-derived neurotrophic factor |
DI water | Deionized Water |
DIV | Day in-vitro |
DMEM | Dulbecco’s Modified Eagle Medium |
DOPA | 3,4-dihydroxy-L-phenylalanine |
DTPDH | Dithiobis (propanoic dihydrazide) |
ECM | Extracellular matrix |
EDC | Ethylcarbodiimide hydrochloride |
EGF | Epidermal growth factor |
FGF2 | Fibroblast growth factor 2 |
FIB-SEM | Focused ion beam scanning electron microscopy |
FTIR | Fourier transform infrared |
GFAP | Glial fibrillary acidic protein |
HBD | Heparin-binding domain |
Hep | Heparin |
HFIP | Hexafluoro-2-propanol |
iPSC | Induced pluripotent stem cells |
IPTG | isopropyl thiogalactopyranoside |
KC | Keratinocyte |
KCM | Keratinocyte growth medium |
KC-NC | Keratinocyte-derived Neural Crest |
KSFM | KC serum-free growth medium |
MAG | Myelin-associated glycoprotein |
MPG | Myelin protein zero |
NCAM | Neural cell adhesion molecule |
NC | Neural crest |
NCSC | Neural crest stem cell |
NRG1 | Neuregulin beta1 |
OD | Optical density |
p75 NGFR | p75 Nerve growth factor receptor |
PCL | poly-ε-caprolactone |
PDA | Polydopamine |
PDGF-BB | Platelet-derived growth factor-BB |
PGA | Polyglycolic acid |
PLA | Polylactic acid |
PLP1 | Proteolipid protein 1 |
PMSF | Phenylmethanesulfonyl fluoride |
PNI | Peripheral nerve injury |
PNS | Peripheral nervous system |
SC | Schwann cell |
TCEP | Tris(2-carboxyethyl) phosphine |
TCP | Tissue Culture Plate |
TMS | Tetramethylsilane |
References
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Forward H2_BamHI | ATATGGATCCGCCATTCCTGCACCAACTGACCTGAAGTTCA |
Reverse H2_HindIII | ATATAAGCTTATGTCCAATCAGGGGCTCGCTCTTCT |
Forward Fragment-1 of HBD-RGD | TTGGACATAAGCTTGGCGGCGGAGGCAGTGGCCGTGGCGATTCCGGCGGCGGAGGCAGTGGCCGTGGCGATTCCGGCGGCGGAGGCAGTGGCCGTGG |
Reverse Fragment-1 of HBD-RGD | TGGTGGTGCTCGAGATTATTAGGAATCGCCACGGCCACTGCCTCCGCCGCCGGAATCGCCACGGCCACTGCCTCCGCCGCCGGAATCG |
Forward Fragment-2 of HBD-RGD | CGATTCCGGCGGCGGAGGCAGTGGCCGTGGCGATTCCGGCGGCGGAGGCAGTGGCCGTGGCGATTCCTAATAATCTCGAGCACCACCA |
Reverse Fragment-2 of HBD-RGD | CCACGGCCACTGCCTCCGCCGCCGGAATCGCCACGGCCACTGCCTCCGCCGCCGGAATCGCCACGGCCACTGCCTCCGCCGCCAAGCTTATGTCCAA |
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Podder, A.K.; Mohamed, M.A.; Tseropoulos, G.; Nasiri, B.; Andreadis, S.T. Engineering Nanofiber Scaffolds with Biomimetic Cues for Differentiation of Skin-Derived Neural Crest-like Stem Cells to Schwann Cells. Int. J. Mol. Sci. 2022, 23, 10834. https://doi.org/10.3390/ijms231810834
Podder AK, Mohamed MA, Tseropoulos G, Nasiri B, Andreadis ST. Engineering Nanofiber Scaffolds with Biomimetic Cues for Differentiation of Skin-Derived Neural Crest-like Stem Cells to Schwann Cells. International Journal of Molecular Sciences. 2022; 23(18):10834. https://doi.org/10.3390/ijms231810834
Chicago/Turabian StylePodder, Ashis Kumar, Mohamed Alaa Mohamed, Georgios Tseropoulos, Bita Nasiri, and Stelios T. Andreadis. 2022. "Engineering Nanofiber Scaffolds with Biomimetic Cues for Differentiation of Skin-Derived Neural Crest-like Stem Cells to Schwann Cells" International Journal of Molecular Sciences 23, no. 18: 10834. https://doi.org/10.3390/ijms231810834