Role of RNAIII in Resistance to Antibiotics and Antimicrobial Agents in Staphylococcus epidermidis Biofilms
Abstract
:1. Introduction
2. Results
2.1. Biofilm Formation in Presence of Oxacillin and Phenolic Compounds
2.2. Construction of S. epidermidis RP62a-ΔRNAIII
2.3. Effect of Vanillin and Oxacillin on S. epidermidis Biofilm
2.4. Real-Time qPCR Analysis of Biofilm-Associated Genes
2.5. Analysis of Change in the Phenotype of the RNAIII-Deficient Strain
3. Discussion
4. Materials and Methods
4.1. Construction of S. epidermidis RP62a-ΔRNAIII
4.2. Bacterial Strains and Culture Conditions
4.3. Antimicrobial Agents and Antibiotics
4.4. Assessment of Biofilm Formation by Crystal Violet Staining
4.5. Determination of the Growth Curve of Treated and Untreated Strains
4.6. Confocal Laser Scanning Microscopy (CLSM)
4.7. Congo Red Agar Analysis
4.8. Phenol-Chloroform RNA Extraction
4.9. Real-Time qPCR
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Minich, A.; Lišková, V.; Kormanová, Ľ.; Krahulec, J.; Šarkanová, J.; Mikulášová, M.; Levarski, Z.; Stuchlík, S. Role of RNAIII in Resistance to Antibiotics and Antimicrobial Agents in Staphylococcus epidermidis Biofilms. Int. J. Mol. Sci. 2022, 23, 11094. https://doi.org/10.3390/ijms231911094
Minich A, Lišková V, Kormanová Ľ, Krahulec J, Šarkanová J, Mikulášová M, Levarski Z, Stuchlík S. Role of RNAIII in Resistance to Antibiotics and Antimicrobial Agents in Staphylococcus epidermidis Biofilms. International Journal of Molecular Sciences. 2022; 23(19):11094. https://doi.org/10.3390/ijms231911094
Chicago/Turabian StyleMinich, Andrej, Veronika Lišková, Ľubica Kormanová, Ján Krahulec, Júlia Šarkanová, Mária Mikulášová, Zdenko Levarski, and Stanislav Stuchlík. 2022. "Role of RNAIII in Resistance to Antibiotics and Antimicrobial Agents in Staphylococcus epidermidis Biofilms" International Journal of Molecular Sciences 23, no. 19: 11094. https://doi.org/10.3390/ijms231911094