DRB2 Modulates Leaf Rolling by Regulating Accumulation of MicroRNAs Related to Leaf Development in Rice
Abstract
:1. Introduction
2. Results
2.1. Phenotypes of rl89 Mutant
2.2. The rl89 Locus Was Mapped to a Putative Gene Encoding DRB2
2.3. Functional Confirmation of OsDRB2 through Complementation and Knockout Assays
2.4. Subcellular Localization of OsDRB2 Protein
2.5. Expression Analyses of the OsDRB2 Gene
2.6. Protein Interaction of OsDRB2 with OsDCL1 and OsDRB1-2
2.7. Expression Analyses of miRNAs and Their Target Genes Associated with Leaf Development
2.8. Expression Analyses of Some Genes Regulating Leaf Shape and Cell Growth
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Determination of Photosynthetic Parameter and Leaf-rolling Index (LRI)
4.3. Measurement of Photosynthetic Pigments
4.4. Histology Analysis
4.5. MutMap Analysis and Marker Development
4.6. Sequence Analysis
4.7. Vector Construction and Rice Transformation
4.8. Histochemical GUS Assay
4.9. Subcellular Localization
4.10. Yeast-Two-Hybrid (Y2H) Assay
4.11. qRT-PCR Analyses
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yuan, Z.; Pan, J.; Chen, C.; Tang, Y.; Zhang, H.; Guo, J.; Yang, X.; Chen, L.; Li, C.; Zhao, K.; et al. DRB2 Modulates Leaf Rolling by Regulating Accumulation of MicroRNAs Related to Leaf Development in Rice. Int. J. Mol. Sci. 2022, 23, 11147. https://doi.org/10.3390/ijms231911147
Yuan Z, Pan J, Chen C, Tang Y, Zhang H, Guo J, Yang X, Chen L, Li C, Zhao K, et al. DRB2 Modulates Leaf Rolling by Regulating Accumulation of MicroRNAs Related to Leaf Development in Rice. International Journal of Molecular Sciences. 2022; 23(19):11147. https://doi.org/10.3390/ijms231911147
Chicago/Turabian StyleYuan, Zhaodi, Jihong Pan, Congping Chen, Yulin Tang, Hongshan Zhang, Jia Guo, Xiaorong Yang, Longfei Chen, Chunyan Li, Ke Zhao, and et al. 2022. "DRB2 Modulates Leaf Rolling by Regulating Accumulation of MicroRNAs Related to Leaf Development in Rice" International Journal of Molecular Sciences 23, no. 19: 11147. https://doi.org/10.3390/ijms231911147