Remodeling of Liver and Plasma Lipidomes in Mice Lacking Cyclophilin D
Abstract
:1. Introduction
2. Results
2.1. Significant Remodeling of the Liver and Plasma Lipidomes Occurred in Response to CypD Deletion
2.2. The Lipid Content Increased Due to CypD Ablation Both in the Liver and Plasma
2.3. Lipid Composition Changed Due to CypD Ablation
2.4. The Level of Several Liver and Plasma Phospholipid Species Showed a Positive Correlation
2.5. CypD Ablation Did Not Result in Abdominal Fat Accumulation
2.6. TEM Investigation Revealed Structural Differences between CypD-/- and WT Hepatocytes
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Lipid Analysis
4.3. MRI Analysis
4.4. Tissue Preparation for Histological and Histochemical Analysis
4.5. Histochemistry
4.6. Electron Microscopy
4.7. Cholesterol Extraction, Sample Preparation and GC-MS Measurements
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Koszegi, B.; Balogh, G.; Berente, Z.; Vranesics, A.; Pollak, E.; Molnar, L.; Takatsy, A.; Poor, V.; Wahr, M.; Antus, C.; et al. Remodeling of Liver and Plasma Lipidomes in Mice Lacking Cyclophilin D. Int. J. Mol. Sci. 2022, 23, 11274. https://doi.org/10.3390/ijms231911274
Koszegi B, Balogh G, Berente Z, Vranesics A, Pollak E, Molnar L, Takatsy A, Poor V, Wahr M, Antus C, et al. Remodeling of Liver and Plasma Lipidomes in Mice Lacking Cyclophilin D. International Journal of Molecular Sciences. 2022; 23(19):11274. https://doi.org/10.3390/ijms231911274
Chicago/Turabian StyleKoszegi, Balazs, Gabor Balogh, Zoltan Berente, Anett Vranesics, Edit Pollak, Laszlo Molnar, Aniko Takatsy, Viktoria Poor, Matyas Wahr, Csenge Antus, and et al. 2022. "Remodeling of Liver and Plasma Lipidomes in Mice Lacking Cyclophilin D" International Journal of Molecular Sciences 23, no. 19: 11274. https://doi.org/10.3390/ijms231911274