Genome-Wide Identification and Functional Characterization Reveals the Pivotal Roles of BnaA8.ATG8F in Salt Stress Tolerance and Nitrogen Limitation Adaptation in Allotetraploid Rapeseed
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification of ATG8s in Brassica Species
2.2. Genomic Distribution, Expansion, and Syntenic Analysis of BnaATG8s
2.3. Phylogenetic Relationships and Evolutionary Selection Pressure of the ATG8 Proteins
2.4. Molecular Characterization of BnaATG8s
2.5. Conserved Domains, Gene Structure, Protein Interaction, and Transcriptional Regulatory Analysis
2.6. Transcriptional Analysis of BnaATG8s under Diverse Nutrient Stresses
2.7. Gene Co-Expression Network Analysis of the Core BnaATG8 Members
2.8. BnaA8.ATG8F May Be Indispensable for The Resistance to Salt Stress and Low Nitrogen Stress
2.9. Effects of BnaA8.ATG8F on Morpho-Physiological and Mineral Nutrients in Rapeseed under Salt Stress
2.10. BnaA8.ATG8F Plays a Key Role in Rapeseed Resistance to Low Nitrate Stress
3. Discussion
3.1. Integrated Bioinformatics Analysis Provided a Comprehensive Insight into the Molecular Characteristics of BnaATG8s
3.2. Transcriptional Analysis Revealed the Response of the BnaATG8 Family to Various Nutrient Stresses and Identified the Core Member BnaA8.ATG8F
3.3. Molecular Strategy Model of BnaA8.ATG8F Regulating Salt Stress Resistance and Nitrogen Limitation Adaptation in Allotetraploid Rapeseed
4. Materials and Methods
4.1. Identification and Gene Nomenclature of Autophagy Gene 8 (ATG8) Family in Brassica
4.2. Chromosomal Localization and Syntenic Analysis of ATG8s in Brassica
4.3. Analysis of Evolutionary Selection Pressure and Phylogeny Relationships
4.4. Identification of Exon-Intron Structures, Conserved Motifs, and Putative Cis-Acting Regulatory Elements (CREs)
4.5. Molecular Characterization of the ATG8 Proteins
4.6. Transcriptional Responses of BnaATG8s and Functionality Analysis under Different Nutrient Stresses
4.7. Differential Expression and Gene Co-Expression Network Analysis
4.8. Construction of Recombinant Vector and Acquisition of Transgenic Rapeseed
4.9. Determination of Osmoregulatory Substances, Chlorophyll, Anthocyanin, Nitrate Nitrogen, Total Nitrogen Concentration, and Enzyme Activities
4.10. Ionomic Analysis
4.11. Quantitative Reverse-Transcription PCR (RT-qPCR) Assays
4.12. Statistical Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Zhang, T.; Zhou, T.; Zhang, Y.; Chen, J.; Song, H.; Wu, P.; Yue, C.; Huang, J.; Zhang, Z.; Hua, Y. Genome-Wide Identification and Functional Characterization Reveals the Pivotal Roles of BnaA8.ATG8F in Salt Stress Tolerance and Nitrogen Limitation Adaptation in Allotetraploid Rapeseed. Int. J. Mol. Sci. 2022, 23, 11318. https://doi.org/10.3390/ijms231911318
Zhang T, Zhou T, Zhang Y, Chen J, Song H, Wu P, Yue C, Huang J, Zhang Z, Hua Y. Genome-Wide Identification and Functional Characterization Reveals the Pivotal Roles of BnaA8.ATG8F in Salt Stress Tolerance and Nitrogen Limitation Adaptation in Allotetraploid Rapeseed. International Journal of Molecular Sciences. 2022; 23(19):11318. https://doi.org/10.3390/ijms231911318
Chicago/Turabian StyleZhang, Tianyu, Ting Zhou, Yifan Zhang, Junfan Chen, Haili Song, Pengjia Wu, Caipeng Yue, Jinyong Huang, Zhenhua Zhang, and Yingpeng Hua. 2022. "Genome-Wide Identification and Functional Characterization Reveals the Pivotal Roles of BnaA8.ATG8F in Salt Stress Tolerance and Nitrogen Limitation Adaptation in Allotetraploid Rapeseed" International Journal of Molecular Sciences 23, no. 19: 11318. https://doi.org/10.3390/ijms231911318