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Review

Root-Related Genes in Crops and Their Application under Drought Stress Resistance—A Review

by
Tianyuan Qin
1,
Ali Kazim
2,
Yihao Wang
1,
Dormatey Richard
1,
Panfeng Yao
1,
Zhenzhen Bi
1,
Yuhui Liu
1,
Chao Sun
1,* and
Jiangping Bai
1,*
1
State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
2
National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Centre, Park Road, Islamabad 45500, Pakistan
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(19), 11477; https://doi.org/10.3390/ijms231911477
Submission received: 31 August 2022 / Revised: 25 September 2022 / Accepted: 26 September 2022 / Published: 29 September 2022
(This article belongs to the Section Molecular Plant Sciences)
Browse Figure
Versions Notes

Abstract

:
Crop growth and development are frequently affected by biotic and abiotic stresses. The adaptation of crops to stress is mostly achieved by regulating specific genes. The root system is the primary organ for nutrient and water uptake, and has an important role in drought stress response. The improvement of stress tolerance to increase crop yield potential and yield stability is a traditional goal of breeders in cultivar development using integrated breeding methods. An improved understanding of genes that control root development will enable the formulation of strategies to incorporate stress-tolerant genes into breeding for complex agronomic traits and provide opportunities for developing stress-tolerant germplasm. We screened the genes associated with root growth and development from diverse plants including Arabidopsis, rice, maize, pepper and tomato. This paper provides a theoretical basis for the application of root-related genes in molecular breeding to achieve crop drought tolerance by the improvement of root architecture.

1. Introduction

Global warming and water scarcity attributable to climate change have caused an urgent global food security challenge. One third of the world’s arable land is undersupplied with water, while other arable land is frequently affected by periodic or unpredictable droughts that have resulted in yield losses of up to approximately USD 30 billion in the last decade [1,2]. As the global population continues to grow (and is expected to increase to approximately 10 billion people by 2050 [3]), the demand for water for agriculture and the reduced availability of freshwater will further exacerbate the impact of droughts on agriculture [4]. Therefore, studying the mechanisms by which plants can sustain growth during droughts, and the exploration of strategies to improve plant survivability may provide solutions to future food security problems [5,6,7]. In China, sustainable agricultural development is severely affected by the large proportion of arid and semi-arid regions, high water consumption in agriculture, and low irrigation water-use efficiency. Irrigation and improvement of the water-holding capacity of soil are effective agronomic measures to compensate for low rainfall, but selection and breeding of drought-tolerant plant cultivars is an additional effective strategy to improve the water-use efficiency of crops. Therefore, plant breeders are targeting leaf growth and transpiration traits to improve plant reproductive phenology under water limitation [8]. However, water uptake and root phenotypic traits, which are the most important factors affecting plant water uptake, have not been adequately addressed by breeders mainly because of the high variability of root systems in relation to the environment and the difficulty of in situ monitoring of root systems. Therefore, understanding the response of root cells to water deficit is crucial for continued sustainable agricultural production [9,10,11].
Potato (Solanum tuberosum L.) is an important tuberous crop and is among the four largest crops in the world. Global potato production is currently about 350 billion kg per year and is increasing annually [12,13]. China is the largest potato producer in the world. Potato-growing areas in China are mainly located in the northwest, western Inner Mongolia, the northeast, and other arid and semi-arid regions with an average annual precipitation less than 500 mm. Stress from prolonged or seasonal drought can severely affect potato plant growth, tuber yield, and marketability. Especially in the tuber growth stage, drought stress can cause significant yield loss or even crop failure [14,15]. Therefore, research on the drought tolerance of potato is essential to ensure productivity under global climate change.
In this review, we compiled 217 published genes associated with root development from diverse plant species, including Arabidopsis, rice, maize, pepper and tomato. Our future aim is to further validate the functions of these genes in potato and apply them in breeding programs to improve potato drought tolerance.

2. Root Traits Associated with Drought Stress Tolerance

The root system plays an essential role in the plant life cycle and forms a complex structure through growth and branching to fulfil its primary functions of anchoring the plant in the soil and absorbing water and nutrients [16,17]. Important structural characteristics of the root system include the length of the primary root, the density of the secondary roots, and the gravitropic set-point angle of the root, all of which are mostly regulated by plant hormones [18,19]. In rice and Arabidopsis, a deep-penetrating root system and positively geotropic root growth are the predominant traits that govern drought adaptation, as both phenotypes facilitate water uptake from deeper layers of the soil and help to ensure that normal plant activities are maintained [20].
In addition to the root architecture, water uptake depends on the intrinsic water-transport capacity of the root system. Normally, water absorbed by the root is radially transferred to the central stele and transported in the xylem to the aboveground organs, where water channel proteins (termed aquaporins) play a vital role in intercellular water transport. The root system responds to changes in soil water availability at the cellular and root architecture levels, and the root system cell ecological niche, phloem tissue, and vascular system coordinate to respond to adverse stresses [21]. When a plant perceives water deficit, the architecture of the root system is subjected to morphological changes through cell division, elongation, and differentiation at the root tip to improve the water uptake capacity. Root system architecture is associated with the distribution and depth of the soil water layer. The deeper the root system, the smaller the branching angle of the root tip, and the more efficiently the root system can absorb water from deeper soil layers [22]. Lignification and suberization of specific walls of the endodermal cells play an important role in water transport in the xylem. The physiological water balance of the plant is actively maintained by water transport in the xylem from the roots to the aboveground organs and photosynthetic assimilate transport in the phloem from the shoots to the root system. The transport process may also affect the drought tolerance of the plant [23].
In general, many traits can be used to screen plant roots for drought tolerance, including the germination capacity of the plant in soils with different osmotic potentials, the depth, width, and thickness of roots in the soil, the starch hydrolysis status of the root crown, and the proline accumulation capacity of the root system under drought, as well as antioxidant enzyme activities and root vitality [24,25,26,27,28]. In addition, morphological variables, such as root growth and yield indicators, can be used to assess drought resistance in crops [29,30]. Therefore, a comprehensive assessment combining the above indicators will more realistically reflect the actual drought resistance of a crop species. Since the root system is the main organ that allows plants to absorb water, it is necessary to comprehensively understand the genes related to root growth and development.

3. Current Status of Research on Genes Associated with Root Growth and Development

Distribution in the soil and degree of development of the root system are directly related to the growth and development of aerial shoots. Most plants depend on the root system to supply the water and minerals essential for the growth and maintenance of the aboveground organs. A balance is established between the development of the branches and leaves and the development of the root system [31,32]. The development of the root system is much greater than that of the aboveground parts, and the total area of the root system in contact with the soil often exceeds the area of the stem and leaves by a factor of 6–16 [33]. The spatial configuration of the root system can be generally divided into crown roots, primary roots, lateral roots, and adventitious roots. Root elongation, water and nutrient uptake, tissue differentiation, and response to gravity and light all occur in the root tip, which consists of the root cap, meristematic zone, elongation zone, and maturation zone. The root cap protects the growing point of the root tip from friction and damage from the soil and is the primary gravity-sensing site. The epidermal cells of the maturation zone project outward to form root hairs. As root hairs are shed and the root tip grows, root hairs develop from newly generated cells; thus, a root hair zone is always maintained at the root tip, which is the most active portion of the root for absorbing water and inorganic salts [34,35,36].
We conducted a literature search and identified 217 published genes associated with different root development traits (Table 1) to investigate the important functions of plant root systems. The major genes that represent root growth and development are shown in Figure 1.

3.1. Genes Associated with Adventitious Root Growth and Development

Adventitious roots are an important component of potato root system architecture (RSA); thus, understanding adventitious root development may be useful to improve potato yield and optimize the potential of agricultural land use [37]. Roots are not only the principal organs for water and nutrient uptake in plants, but they also respond to environmental and plant–soil microbial interactions [38,39]. Rice, an important food crop, has a fibrous-rooted system. The subsequent growth of the rice root system depends on adventitious roots that continuously emerge from the germinal sheath or the base of stem nodes. Scientists such as Zhou Daoxiu and Zhao Yu have been studying rice epigenetics and the mechanisms that control the development of adventitious roots in rice since as early as 2009. They identified the WUSCHEL-Related Homeobox 11 (WOX11) gene from the WUSCHEL-related homeobox domain family, an important regulator of adventitious root development in rice. WOX11 is specifically expressed in the meristematic zone of adventitious roots in rice after elongation. Overexpression of WOX11 not only increases the number of adventitious roots but can also give rise to ectopic roots on the rice stems and at the base of the flower. Reduced expression or the complete loss-of-function of this gene results in a dramatic reduction in the adventitious root phenotype. Further studies revealed that WOX11 directly represses the expression of Ribonucleotide Reductase 2 (RR2), encoding an A-type cytokinin response factor that participates in the development of adventitious roots in rice [40]. WOX11 and the AP2-like transcription factor ERF3 (EUKARYOTIC RELEASE FACTOR 3) precisely regulate the expression of RR2 in a reciprocal manner to control the initiation and elongation of adventitious roots in rice [41]. The genes ZmRTCS and ZmRTCL are important regulators of adventitious root formation in maize and both RTCS (rootless concerning crown and seminal roots) and RTCL (RTCS-like) proteins bind to the LBD (lateral organ boundaries domain) downstream promoter response element ARF34 and function as transcription factors. Mutation of RTCL leads to the early growth and developmental arrest of adventitious roots in maize, and RTCS regulates transcriptional expression of the RTCL gene in the maize root, demonstrating the synergistic roles of RTCS and RTCL in adventitious root formation [42].
In Arabidopsis, PIN-FORMED (PIN) polarity regulators have been studied by mutagenizing the PIN2:PIN1-HA; pin2 strain and identifying the regulator of PIN polarity 12 (repp12) mutant, which restored the gravitropic growth phenotype of the Arabidopsis adventitious root system [43,44]. Similarly, a study on rice showed that OsPIN is expressed in vascular tissue and root primordia in a manner similar to Arabidopsis AtPIN1. In transgenic rice, in which OsPIN1 was silenced by RNA interference (RNAi), root emergence and development are significantly inhibited. Overexpression or suppression of OsPIN1 expression by transgenic methods result in significant changes in the number of tillers and the root-to-shoot ratio, suggesting that OsPIN1 is important in rice root growth and tillering [45]. In addition, a novel regulator of adventitious root development in rice, CROWN ROOT DEFECT 1 (CRD1), was identified by screening a rice mutant library. Zhu et al. [46] revealed that CRD1 affects adventitious root development in rice by regulating the development of adventitious root primordia. Small RNA sequencing of wild type and the mutant revealed that CRD1 can regulate miR156 levels, thereby modulating adventitious root development. In addition, the authors showed that CRD1 can maintain miRNA stability and they demonstrated its essential role in adventitious root development in indica and japonica rice cultivars. Several mutant tomato lines have been generated by CRISPR/Cas9-mediated editing of a Cas9/single-guide RNA construct targeting the second exon of CCD8 (CCD8Cas9). The T1 plants of the CCD8Cas9 mutant exhibited several morphological changes including dwarfism and formation of excessive adventitious roots [47]. Among four potato cultivars differing in earliness, drought reduced the maximum dry mass of roots and the total length of stolons, but increased stolon number. The number of adventitious roots on stolons was decreased under drought stress and was negatively correlated with the root dry mass of plants. Fresh tuber yield was significantly correlated with root dry mass in the field, and the drought tolerance index was significantly correlated with root depth in the field [48,49].

3.2. Genes Associated with Primary Root Growth and Development

The primary root, which develops from the radicle, has a strong growth ability and can grow to a depth of 2–3 m. In a study of Arabidopsis, Jia et al. [50] used genome-wide association analysis to identify genes associated with changes in primary root length. The authors identified BRASSINOSTEROID-SIGNALING KINASE 3 (BSK3) as the main gene effecting primary root length. In addition, the basic helix-loop-helix (bHLH) transcription factor UPBEAT1 (UPB1) regulates the balance between cell proliferation and differentiation by directly controlling peroxidase expression [50]. The differential localization of UPB1 in transcriptional and translational reporter gene lines of Arabidopsis suggests that deletion mutants of UPB1 lead to impaired growth of the primary root. The transcriptional regulator may also function as an intercellular signaling molecule and provide a direct transcriptional link between the distribution of reactive oxygen species (ROS) and the proliferation state of root apical cells [51]. In Arabidopsis, overexpression of HOOK-ASSOCIATED PROTEIN 3B (HAP3b) promotes elongation of the primary root. Root cells overexpressing HAP3b elongate faster than wild-type root cells, and HAP3b is specifically expressed in the apical region and promotes apical cell division and elongation [52].
Two heterologous genes, MAINTENANCE OF MERISTEMS (MAIN) and MAINTENANCE OF MERISTEMS LIKE 1 (MAIL1), in Arabidopsis encode a conserved retrotransposon-associated mobile plant domain essential for primary root development [53]. Loss-of-function of MAIN or MAIL1 results in the release of heterochromatin, reduced cohesion of heterochromatin around metaphase, cell death of meristematic tissue, and growth arrest shortly after primary root emergence. In addition, loss-of-function of PROTEIN PHOSPHATASE 7-LIKE (PP7L) results in the same root growth phenotype as the loss-of-function main or mail1 mutants, with the PP7L mutant showing an incipient root growth arrest phenotype. A double-mutation analysis confirmed that the three genes, MAIN, MAIL, and PP7L act in the same molecular pathway [54]. ROOT MERISTEM GROWTH FACTOR 1 (RGF1), a secreted peptide hormone, assists/regulates phloem development in Arabidopsis primordial roots. RGF1 regulates root phloem tissue activity mainly through two downstream transcription factors, PLETHORA 1 (PLT1) and PLT2. Two independent rgi1 rgi2 rgi3 rgi4 rgi5 quintuple mutants exhibit a consistent short primary root phenotype. Expression of PLT1 and PLT2 is barely detectable in the quintuple mutants. Ectopic expression in the quintuple mutant of PLT2 driven by the RGF1 INSENSITIVE 2 (RGI2) promoter largely reverses the primary root meristem defects. Exogenous administration of RGF1 rapidly and simultaneously induces phosphorylation and ubiquitination of RGI1, which enables RGI1 to recognize and transduce peptide signals from RGF1. Thus, RGIs function as receptors for RGF1 and regulate meristem development in Arabidopsis primordial roots [55].
In rice, two T-DNA insertion mutant strains of ROOT LENGTH REGULATOR 4 (OsRLR4) developed primary roots longer than those of the wild type, whereas the opposite was true for the overexpression strains. Inukai et al. [56] characterized five recessive mutants in rice, BRX65, BRX117, BRX430, BRX448 and crl2 mutants, to analyze the genetic mechanisms controlling root elongation. The mutants, which showed a short root length phenotype, were genetically defined as reduced root length (rrl). The first rrl locus was designated as RRL1 (BRX65), the other locus as RRL2, and the alleles were designated as RRL2-1 (BRX117), RRL2-2 (BRX430), and RRL2-3 (BRX448). In the rrl1 mutant, only the mature cortical cells were significantly shorter than in the wild type, whereas the roots of the rrl2-1 mutant had significantly shorter cell length, apical meristem size, and cell flux than the wild type. However, mature cortical cell length, apical meristem size, and cell flux were significantly higher in roots of the crl2 mutant than in the wild type. The rrl1 crl2 double mutant had a mature bark cell length intermediate between the parental single mutants, whereas the rrl2-1 crl2 double mutant had cell length, meristem size, and cell flux intermediate between the parental single mutants. These results suggest that opposing effects of these genes determine the extent of primary root growth and development in rice [57]. In addition, the OsAGP gene in rice encodes a protein with a structure similar to that predicted by ArfGAP. The purified OsAGP-GST fusion protein is able to stimulate the GTPase activity of rice Arf. In addition, OsAGP is able to repair the defective vesicular translocation in yeast cells of the gcs1∆glo3∆ double mutant. Transgenic Arabidopsis constitutively expressing OsAGP shows reduced apical dominance and shortened primary roots. These results suggest that rice ARF-GAP may be involved in regulating plant root growth and development [58].
A receptor gene for Glu, designated as GLR3, has a T-DNA insertion that results in a mutant phenotype of shortened rice primary roots. Histological and DNA synthesis analyses showed that the activity of the mutant root meristem is impaired and associated with enhanced programmed cell death. Thus, in rice, GLR3 is essential for the maintenance of cell division and cell survival in the early root tip meristem tissue of seedlings [59]. Similarly, a short postembryonic root mutant of rice, designated as OsSPR1, has shorter postembryonic roots and encodes a novel mitochondrial protein with an armadillo-like repeat structural domain. Complementation experiments with the spr1 mutant have confirmed the involvement of the OsSPR1 mutant in the elongation growth of rice postembryonic roots [60]. The rice mutant Osglu3-1, with a short root phenotype caused by a point mutation in OsGLU3, which encodes a membrane-bound protein, was isolated and identified from a rice library mutagenized with ethyl methanesulfonate (EMS). The Osglu3-1 mutant has less crystalline cellulose in the root cell wall, shorter root cells, and less root meristem tissue. OsGLU3 is widely expressed in various tissues, and especially strongly in the roots, and regulates root elongation by changing the cell wall cellulose content [61].
Mapping-based cloning revealed that an EMS-mutagenized rice mutant with short roots was caused by a point mutation, causing premature termination during protein synthesis in an intron of OsDGL1. OsDGL1 is a direct homolog of Arabidopsis DGL1 and yeast WBP1 and is involved in N-glycosylation in eukaryotes. The Osdgl1 rice mutant exhibits alternations in the polysaccharides of the root cell wall matrix, resulting in shortened root cells, less root meristem tissue, and cell death in the root [62].
AUXIN RESISTANT 1 (AUX1) and PIN2 regulate root gravitropism. The aux1-T mutant shows stronger defects in root gravitropism than the pin2-T aux1-T double mutant. The pin2-T double mutant shows a similar phenotype to aux1-T. No asymmetric distribution of gravity-induced growth hormone response is detectable in the pin2-T, aux1-T, and aux1-T pin2-T mutants. In contrast, the aux1-T pin2-T double mutant shows similar growth hormone responses to the aux1-T mutant, indicating that AUX1 controls gravitropic root growth by regulating the asymmetric distribution of growth hormone upstream of PIN2 [63]. Diallyl disulfide (DADS) has significant effects on tomato seed germination, root growth, mitotic index, root meristem cell size, and hormone content, as well as expression of growth hormone biosynthesis genes (FZYs), growth hormone transport genes (SlPINs), and expansin genes (EXPs) in the root system. Low concentrations (0.01–0.62 mM) of DADS promote root growth, whereas high concentrations (6.20–20.67 mM) have inhibitory effects. The expression levels of EXP1, EXB2, EXB3, EXLB1, and β-expanding protein precursors are increased by DADS treatment. These results suggest that tomato root growth is regulated by a variety of expander protein genes at different stages of tomato development [64]. A cell wall-associated receptor kinase (WAK) directly links the extracellular matrix to the intracellular compartment and is involved in developmental processes and stress responses. In barley, HvWAK1 is specifically expressed in the roots. Significant differences in root growth have been observed between wild-type and HvWAK1-mutant seedlings of barley under control and salt stress environments [65,66].

3.3. Genes Associated with Lateral Root Growth and Development

Root branching is an important factor in root architecture. Lateral roots improve root attachment, water and nutrient uptake, and influence plant growth and development in response to various environmental signals in the soil. Initiation of lateral roots begins with specific mesocolonial sheath cells, that undergo a series of tightly coordinated asymmetric cell divisions to form the lateral root primordia [67]. Initiation of lateral roots is caused by the cyclic death of lateral root cap cells, resulting in a shock to growth hormone signaling in the elongation zone. These cells, characterized by growth hormone signaling, can give rise to a pre-branching site with the ability to form lateral root primordia [68,69]. The formation of the lateral root primordia is characterized by the coordinated nuclear migration of two or three adjacent polar pericycle cells of the xylem, followed by a series of well-organized cell divisions. The involvement of specific molecules is required for different steps of the process before or during lateral root formation [70].
In Arabidopsis, GOLVEN 6 (GLV6), a member of the GOLVEN/root growth factor/CLE-like (GLV/RGF/CLEL) signal peptide family, is involved in lateral root initiation. Loss-of-function mutation of the related gene GLV10 results in increased asymmetric cell division during lateral root initiation. The leucine-rich repeat-like receptor kinases RGI1, 4, and 5 may function as recognition receptors for GLV6 during lateral root initiation. A GLV6 inhibitor screening revealed that the mitogen-activated protein kinase MPK6 may function as a direct downstream signal for GLV6. GLV6/10 inhibits asymmetric cell division via the RGF1-insensitive receptor and MPK6 signaling pathway to limit the initial asymmetric cell division that occurs during lateral root initiation. During lateral root initiation, a series of initial asymmetric cell divisions occur [71].
The LBD transcription factor is among the most intensively studied downstream target genes of ARF7, which is mainly expressed in lateral root primordia and adjacent cell tissues and regulates lateral root development during a novel mechanism for the regulation of lateral root development in Arabidopsis [72]. A novel downstream target gene in the ARF7-LBDs-mediated growth hormone signaling pathway, named PR-1 HOMOLOG 1 (PRH1), is involved in the regulation of growth hormone-induced lateral root development in Arabidopsis downstream of ARF7 and LBDs. Overexpression of PRH1 in an arf7 background partially restored the lateral root phenotype of ARF7. LBDs, which are also located downstream of ARF7, are involved in regulating the transcriptional expression of PRH1. Overexpression of PRH1 partially restored the reduced lateral root phenotype in lbds mutants [73,74,75].
Lateral root genesis in Arabidopsis begins with the asymmetric division of the guard cell, which undergoes cell proliferation and differentiation to form a new lateral root primordium and eventually new meristematic tissue [76,77]. Genetic and physiological analyses have shown that most developmental events during lateral root formation are regulated by growth hormone signaling [68]. The transcription factors GATA23 and LBD16 have been identified as important growth hormone-inducible transcription factors in lateral root formation cells, and LBD16 can be directly activated by the SLR/IAA14–ARF7–ARF19 signaling pathway [78,79]. Based on the genetic identification of growth hormone signaling pathways involved in lateral root initiation, ARF7 activates LBD16, which is upstream of the AP2/EREBP-like transcription factor PUCHI (a regulator of lateral root primordia) [80,81]. This suggests that spatiotemporal control of PUCHI expression by LBD16 is important for promoting lateral root formation and that initiation of lateral roots requires sequential induction of the LBD16 and PUCHI transcription factors [82].
The adaptation of plant roots to environmental stresses depends largely on the growth and development of lateral roots. In chrysanthemum, CmANR1, a homolog of Arabidopsis AtANR1, plays a critical role in regulating lateral root development. Ectopic expression of CmANR1 in Arabidopsis significantly increases the number and length of lateral roots compared with the wild type. Moreover, CmANR1 promotes lateral root growth and development by regulating growth hormone biosynthesis and transport [83]. A novel mechanism has been observed by which CmANR1 in chrysanthemum can transcriptionally activate expression of the growth hormone transport gene CmPIN2 and increase the content of growth hormones in the root system, thereby promoting root development. Hydroponically grown transgenic chrysanthemum CmANR1 has a more extended and stronger root system. In addition, the number and total length of lateral roots and the total volume of the root system are significantly increased compared with wild-type chrysanthemum, suggesting that CmANR1 may promote lateral root development in chrysanthemum. Furthermore, CmANR1 promotes lateral root development in chrysanthemum by increasing auxin contents in the root system [84]. In plants, growth hormone distribution is closely associated with lateral root development. Recent research has also shown that auxin activates mitogen-activated protein kinases (MAPKs) via transverse membrane kinases (TMKs) to control the pattern of cell division during lateral root development. Both TMK1/4 and MKK4/5-MPK3/6 signaling pathways are required to control cell division, which ultimately determines lateral root development in response to auxin. In addition, TMKs directly and specifically interact with phosphorylate MKK4/5, which is required for activation of the MKK4/5-MPK3/6 pathway by auxin. Thus, TMK-mediated growth hormone signaling promotes lateral root growth and development by regulating cell division patterns via MAPK signaling [85].
Maize deeper rooting 1 (DRO1) is negatively regulated by auxin and is involved in the elongation of root tip cells, resulting in asymmetric lateral roots and downward bending under gravity. Overexpression experiments have shown that the higher the expression level of DRO1, the greater the angle of lateral root growth and the more the depth of growth [19]. Similarly, DRO1 influences the direction of lateral root growth. The promoter–reporter structure shows that mutations in AtDRO1 can lead to greater horizontal entrapment of lateral roots, and overexpression of PpeDRO1 in plum results in a deeper root phenotype. These data suggest that DRO1-related genes play an important role in altering root architecture [86]. In Arabidopsis, a family of six genes share five regions of limited sequence similarity with the LAZY1 gene, and a gene in rice is involved in early gravitropic signaling of lateral root gravitropism. Insertion of T-DNA into the Arabidopsis gene AT5G14090, which is most similar to LAZY1, indicated that AtLAZY1 is able to regulate the lateral root meristem phenotype in Arabidopsis [87].
KNOTTED-LIKE FROM ARABIDOPSIS THALIANA 1 (KNAT1) is involved in regulating the angle of lateral roots in Arabidopsis when grown on vertical and inclined agar medium. The two mutant alleles (bp-1 and bp-5) have roots that are excessively inclined to the right side of gravity when grown on a vertical and inclined agar medium surface. Further investigation revealed that the knat1 mutation significantly reduces growth hormone transport in roots and increases growth hormone accumulation in roots. This change in growth hormone transport is accompanied by a reduction in PIN2 concentration in the root tip, as determined by PIN2-GFP reporter and Western blot analysis. These results suggest that KNAT1 may negatively affect the gravitropic angle of lateral roots by regulating growth hormone transport [88]. Four rice mutants with different genetic backgrounds show a reduced number of lateral roots and partial loss of gravitropism. Positional cloning of one of the four mutants revealed that this was due to a loss-of-function of the ribosylation factor guanine nucleotide exchange factor (OsGNOM1) ADP. In addition, the expression of OsPIN2, OsPIN5b, and OsPIN9 is altered in the mutants [89]. OsIAA3 is an IAA gene family member, for which the expression level increases rapidly in response to growth hormone. Overexpression of OsIAA3 in rice results in phenotypic traits such as insensitivity to growth hormones and gravitropic stimulation, as well as reduced lateral root formation and abnormal leaf formation [90].

3.4. Genes Associated with Root Hair Growth and Development

Root hairs are mainly located in the maturation zone of the root tip and are formed as protrusions from the epidermal cells. Root hairs increase the absorption area of the root and secrete acidic substances that promote the dissolution of salts in soil and thus increase nutrient uptake by the plant [91]. The length of root hairs can vary by as much as 100-fold, and therefore are an excellent system for studying the cellular regulatory mechanisms of the plant root system.
Initiation of root hair growth is regulated by several bHLH transcription factors, such as Root Hair Defective 6-like 4 (RSL4), RSL2, and Root Hair Defective 6 (RHD6) [92]. Several growth and developmental regulatory pathways, as well as signals from the growth hormones ethylene and abscisic acid, are important regulators of root hair elongation [93]. In addition, ROS can regulate root hair elongation by oxidizing cell wall-specific components and affecting cell wall cross-linking and hardening [94]. M190905 is located in the intron region of the peroxidase gene PEROXIDASE 62 (PRX62). An additional peroxidase gene, PRX69, is highly expressed in root hairs [95]. The FAB1 protein and its product PtdIns (3,5) P2 are localized to the cell membrane of the root hair stalk. Phenotypic analysis after the simultaneous reduction of FAB1A/FAB1B expression in artificial microRNA mutants revealed that the root hairs were shorter, wider, wavier, and formed branches. The reduction in FAB1A/FAB1B expression also affects the thickness of secondary cell walls in the xylem and the microtubule arrangement in root hairs. Subsequent biochemical experiments have revealed that RHO-RELATED GTPASES FROM PLANTS 10 (ROP10) is involved in sclerotization of the root hair stalk. Furthermore, ROP10 interacts with FAB in root hairs and the cell membrane localization of ROP10 is dependent on the kinase PtdIns (3,5) P2 [96].
A zinc finger protein 5 (zfp5) mutant and zfp5 RNAi strains show reduced ZFP5 function, resulting in fewer and shorter root hairs compared to the wild type. ZFP5 affects root hair development by directly promoting the expression of CAPRICE (CPC) [97]. Root hair formation in Arabidopsis is mainly controlled by a transcriptional activation complex that induces the homologous gene GL2 and MYB genes with a single repeat R3 to regulate root hair development. The gl2 single mutant partially restores the phenotype of a lack of root hairs. The double and higher mutant between gl2 and a myb single mutant have a similar root hair phenotype to the gl2 single mutant. These results suggest that gl2 and a single myb function in a common pathway to regulate the root hair pattern [98]. Similarly, WRKY DNA BINDING PROTEIN 75 (WRKY75) inhibits the growth and development of Arabidopsis root hairs and represses expression of TRIPTYCHON and CPC. A yeast one-hybrid assay showed that the WRKY75 protein bound to the CPC promoter. The WRKY75 gene is mainly expressed in the mesocolonial sheath and vascular tissue [99]. In addition, Arabidopsis mutants with an altered root hair phenotype have been used to study cell wall dynamics and the expression of S-Nitrosoglutathione (GSNO) in roots during the induction of root hair formation. GSNO and growth hormone were jointly involved in the restoration of the root hair phenotype in the root-hairless rhd6 mutant. GSNO regulated the expression of a large number of genes associated with cell wall composition and metabolism, as well as genes encoding ribosomal proteins, DNA- and histone-modifying enzymes, and proteins involved in post-translational modifications [100].
In the rice mutant rth1, root hair elongation is eliminated after swelling. Sequence comparison of three genes from the wild type and the rth1 mutant revealed a nucleotide substitution in OsAPY only. This nucleotide substitution results in abnormal splicing of the cDNA sequence in the rth1 mutant. Following introduction of the OsAPY allele, the transgenic plants developed normal root hairs and showed the complementary phenotype of the rth1 mutant. This suggests that OsAPY may directly regulate root hair growth and development in rice [101]. The cellulose synthase-like gene OsCSLD1 is required for root hair growth and development, and rice mutants defective for OsCSLD1 exhibit an abnormal root hair phenotype or lack root hairs. Gene expression analysis and an in situ hybridization analysis showed that OsCSLD1 is expressed only in root hair cells. OsCSLD1 is the only gene among four OsCSLD genes that exhibits root-specific expression [102]. Yuo et al. demonstrated the molecular mechanisms involved in the specific expression of OsCSLD1 in root hairs and the growth and development of rice root hairs [103]. A rice T-DNA mutant with short root hairs, designated as ossrh3 (oryza sativa short root hair 3), exhibits severely impaired root hair elongation together with changes in plant height, main root length, lateral root length, and number of lateral roots. Genetic analysis showed that the mutant trait is controlled by a single pair of recessive genes, and OsSRH3 has been identified by molecular marker and localization analyses to be responsible for root hair development in rice [104,105,106].
A gene encoding EXPANSIN A17 (EXPA17) was identified in a rice mutant with short root hairs. The mutant OsEXPA17 protein contains a point mutation that results in a change in amino acid sequence. Further studies in which OsEXPA17 expression was inhibited by RNAi confirmed that OsEXPA17 was capable of regulating root hair elongation in rice. Complementation of the OsEXPA17 mutant with the root hair-expressed genes OsEXPA30 and AtEXPA7 in Arabidopsis restored root hair elongation, suggesting that members of the root hair expansion protein subclass play a crucial role in root hair formation [107,108]. In maize, root hairs of the recessive root-hairless mutant rth6 bulged at the transition to root tip growth, and then stopped growing and exhibited a rough cell surface. A phylogenetic analysis showed that ROOTHAIRLESS 6 (RTH6) belongs to the D-type cellulose synthase branch, which is found only in monocotyledons, and that D-type cellulose synthase is highly conserved in the plant kingdom, with five gene family members in maize. Expression profiling showed that RTH6 is highly enriched in root hairs compared with other root tissues [109].

4. Conclusions

Declining crop yields caused by abiotic stresses pose a major challenge to the production of staple crops, and global demand for food is expected to exceed genetic advances in the near future. Thus, environmental changes pose a significant risk to food security. Therefore, integrated solutions are needed to address these challenges and increase crop yields to ensure food security. Plant roots are the first organs to sense drought stress and their morphological structure plays a pivotal role in coping with drought stress [27,110]. When plants are under drought stress, the number of root branches and root hairs increases as the root system becomes more deeply rooted, maximizing the uptake and utilization of nutrients and water in the soil. Therefore, plant root architecture is an important phenotypic indicator of drought resistance in crops [111]. In recent years, studies on the root system of tuber crops have shown that root architecture is strongly correlated with crop yield [112,113]. Exploring the relationship between root architecture and abiotic interaction holds great potential to provide answers to pressing problems, such as root plasticity and crop improvement. As a typical shallow-rooted tuber crop, potato roots penetrate the soil poorly and are sensitive to drought throughout the growing season [114]. The improvement of root architecture is an important direction in breeding potato for drought stress resistance because it can promote faster growth rates, smaller angles of lateral root branches and an increase in the number of root hairs. Thus, a greater number of lateral roots and root hairs can penetrate deeper into the soil to absorb water from lower soil layers under drought stress. Therefore, it is important to explore genes involved in root development in model plants and other crops, such as rice and maize, to identify and screen their homologs in potato to perform functional validations and in-depth studies to improve the root architecture of potato.
Investigation of the root development-related genes discussed in this review will enrich our understanding of root architecture establishment and abiotic stress resistance and expand our knowledge of gene function in improving plant productivity. Breeding stress-resistant plants with high yields will lead to crop improvement. Elucidating the molecular mechanisms of root development genes will provide insights into the different genes and their specific roles in adaptation to various abiotic stresses, thereby providing a better understanding of the targeted opportunities for crop improvement and drought tolerance breeding.

Author Contributions

Conceptualization, T.Q.; methodology, T.Q. and C.S.; investigation, T.Q. and A.K.; data curation, T.Q., C.S. and J.B.; writing—original draft preparation, T.Q.; writing—review and editing, C.S., A.K., Y.W., D.R., P.Y., Z.B., Y.L. and J.B. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Natural Science Foundation of China (Grant No. 32060502 and 31960442), China Agriculture Potato Research System of MOF and MARA (CARS-09-P10), the Gansu Science and Technology fund (Grant No. 21JR7RA804 and 19ZD2WA002-02), and Scientific Research Startup Funds for Openly-recruited Doctors of Gansu Agricultural University (GAU-KYQD-2021-11).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript or in the decision to publish the results.

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Ijms 23 11477 g001 550
Figure 1. Major genes related to root system growth and development.
Figure 1. Major genes related to root system growth and development.
Ijms 23 11477 g001
Table
Table 1. Genes related to the development of different parts of plant roots.
Table 1. Genes related to the development of different parts of plant roots.
Root TypePlant SpeciesGenes
Adventitious rootArabidopsis thalianaPIN1/3, ARF7/17/19, MDR1, SHR, SOS3, WOX5/12
Oryza sativa L.PIN1/2, RAA1, AGAP, GNOM1, MT2b, NAL1, PIN3t, RCC3, CRL1, CAND1, LOB16, CKX4, IAA3, TIR1, WOX11
Zea mays L.RTCL1, RTCS1, LOB6, RTCS
Solanum lycopersicumCCD8
Capsicum annuum L.CaNAC46, CaDSR6
Gossypium hirsutumPIN2
Lateral rootArabidopsis thalianaGLV6/10, ARF7/19, EXP4, ASA1, GATA23, PIN3, SCR, PLT1/2, BRX, PYL8, ALF4, DRO1, LAZY1, KNAT1, ABI3/4, AFB2/3, AGL21/44, ALF1/3/4, ARF7/8/19, AUX1, AXR1/4, BARK1, CEG, CRF2/3, DFL1, E2FA, EIR1, ETR1, FUS3, GNOM, IAA18/19/28, IQM3, KNAT1/3, KRP1/2, LAX3, LBD13/14, LEC2, MDR1, MIZ1, MKK6, MPK13, MUL, MUS, MYB44, NAC1/2, PGP1/4, PHB, PHV, PIN3/7, PLC5, PRE3, PUCHI, PYL8/9, REV, RML2, SGT1B, SHR, SOS3, SWP1, TIR1,WOX7/9, WRKY46, XBAT32, YUCCA4
Oryza sativa L.GNOM, CYP2, ORC3, MT2b, AUX1, NAC9/10, IAA3/11/13, LAZY1, DRO1, CML16, EXPA8, GRXC12, MADS25, NAR2.1, RCc3, WRKY28
Zea mays L.NAC1, Rum1, ARF34, LA1, IRT1, SLR1/2
Gossypium hirsutumNAC9, EXP4
Glycine maxLBD12, WRKY13, NAC1, EXPB2, WNK1
Triticum aestivum L.WRKY51
Solanum lycopersicumEXP4, IAA7, ARF2, DGT, MBP9
Primary rootArabidopsis thalianaKNAT5, HAP3b, DGL1, MAIN, MAIL, RGS1, RGF1, PERK4, ABA2, AFB3, AGL12/14, ARF2, CKX7, DWF4, EIN3, ERF1, GNOM, HYD1, IQM3, MAIL1, MED12, MRP5, PERK4/8, PIN1/2, PLC5, RML1/2, SHR, WOX9/14, UPB1, BSK3
Oryza sativa L.GNA1, GLU3, SPR1, PIN3t, AGAP, ARF12, GLR3.1, DGL1, SOR1, AGAP, AKT1, CML16, CRL2, DGL1, EXPA8, MADS25, MOGS, RAA1, RCc3, RRL1/2
Zea mays L.AUX1, EXPB2
Solanum lycopersicumEXP1, EXPB2
Glycine maxWAK1
Root hairArabidopsis thalianaCOW1, RHD6, IAA7, CTR1, BRI1, AKT1, AXR2, COW1, CPC, CPL3, ETC1/2, EXPA7, FH8, GL1, GLV4, HDG11, IAA17, KOJAK, LRX1/2, MED12/13, MRH1, PERK13, PGP4, PLC5, PRP3, RHD1, ROP2, SOS4, TIP1, TRH1, TTG, WRKY75, ZFP5
Oryza sativa L.APY2, CSLD1, EXPB5, RHL1, SRH1/3, EXP17, YUCCA1, EXPA30, FH1, NOX3, XXT1
Zea mays L.RTH1/2
Hordeum vulgare L.EXPB1/7
Gossypium hirsutumEXPA4
Triticum aestivum L.RSL2/4
Solanum lycopersicumLKT1, SlTRY, SlGL3
Glycine maxEXPB2
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Qin, T.; Kazim, A.; Wang, Y.; Richard, D.; Yao, P.; Bi, Z.; Liu, Y.; Sun, C.; Bai, J. Root-Related Genes in Crops and Their Application under Drought Stress Resistance—A Review. Int. J. Mol. Sci. 2022, 23, 11477. https://doi.org/10.3390/ijms231911477

AMA Style

Qin T, Kazim A, Wang Y, Richard D, Yao P, Bi Z, Liu Y, Sun C, Bai J. Root-Related Genes in Crops and Their Application under Drought Stress Resistance—A Review. International Journal of Molecular Sciences. 2022; 23(19):11477. https://doi.org/10.3390/ijms231911477

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Qin, Tianyuan, Ali Kazim, Yihao Wang, Dormatey Richard, Panfeng Yao, Zhenzhen Bi, Yuhui Liu, Chao Sun, and Jiangping Bai. 2022. "Root-Related Genes in Crops and Their Application under Drought Stress Resistance—A Review" International Journal of Molecular Sciences 23, no. 19: 11477. https://doi.org/10.3390/ijms231911477

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