Selenium-Stimulated Exosomes Enhance Wound Healing by Modulating Inflammation and Angiogenesis
Abstract
:1. Introduction
2. Results
2.1. Selenium Treatment Improved AdMSC Functionality
2.2. AdMSC-Derived Exosomes and Selenium-Stimulated Exosomes Showed Similar Characteristics
2.3. Selenium-Treated Exosomes Strongly Promoted Cell Proliferation by Reducing p16, p21, and Intracellular ROS Accumulation
2.4. Selenium-Treated Exosomes Suppressed the Inflammatory Response by Regulating Pro-Inflammatory and Anti-Inflammatory Factors
2.5. Selenium-Treated Exosomes Accelerated Endothelial Cell Angiogenesis In Vitro
2.6. Selenium-Treated Exosomes Promoted Migration and Wound Closure by Upregulating the Factors Associated with Remodelling
2.7. Selenium-Treated Exosomes Contained Many Beneficial miRNAs for Wound Healing
2.8. Selenium-Treated Exosomes Improved Wound Healing In Vivo
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Cell Proliferation Assay
4.3. Quantitative Reverse Transcription PCR (qRT-PCR)
4.4. Exosome Isolation and Characterization
4.5. Western Blotting
4.6. ROS Analysis
4.7. Multiplex Supernatant Cytokine Assay
4.8. Tube Formation Assay
4.9. In Vitro Wound Healing Assay
4.10. In Vivo Wound Healing Assay
4.11. Library Preparation and Sequencing
4.12. Statistical Analysis
Supplementary Materials
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Heo, J.S. Selenium-Stimulated Exosomes Enhance Wound Healing by Modulating Inflammation and Angiogenesis. Int. J. Mol. Sci. 2022, 23, 11543. https://doi.org/10.3390/ijms231911543
Heo JS. Selenium-Stimulated Exosomes Enhance Wound Healing by Modulating Inflammation and Angiogenesis. International Journal of Molecular Sciences. 2022; 23(19):11543. https://doi.org/10.3390/ijms231911543
Chicago/Turabian StyleHeo, June Seok. 2022. "Selenium-Stimulated Exosomes Enhance Wound Healing by Modulating Inflammation and Angiogenesis" International Journal of Molecular Sciences 23, no. 19: 11543. https://doi.org/10.3390/ijms231911543