HOTAIR/miR-203/CAV1 Crosstalk Influences Proliferation, Migration, and Invasion in the Breast Cancer Cell
Abstract
:1. Introduction
2. Results
2.1. High Expression of HOTAIR in Breast Cancer
2.2. Up-Regulation of HOTAIR Promotes the Proliferation, Invasion, and Migration of MDA-MB-231 Cells
2.3. HOTAIR Binds to miR-203 and Acts as a Molecular Sponge for miR-203
2.4. HOTAIR Reverses the Effect of miR-203 on MDA-MB-231 Cells in Proliferation, Invasion, and Migration
2.5. miR-203 Binds to CAV1 and Inhibits the Expression of CAV1
2.6. miR-203 Restrains the Function of CAV1 on MDA-MB-231 Cells in Proliferation, Invasion, and Migration
3. Discussion
4. Materials and Methods
4.1. Database Search
4.2. Cell Culture
4.3. Cell Transfection
4.4. RNA Extraction and Quantitative Real-Time PCR (RT-qPCR)
4.5. Cell Proliferation Assay
4.6. Cell Migration Assay
4.7. Cell Invasion Assay
4.8. Dual-Luciferase Reporter Assay
4.9. Western Blot
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shi, F.; Chen, X.; Wang, Y.; Xie, Y.; Zhong, J.; Su, K.; Li, M.; Li, Y.; Lin, Q.; Zhou, Y.; et al. HOTAIR/miR-203/CAV1 Crosstalk Influences Proliferation, Migration, and Invasion in the Breast Cancer Cell. Int. J. Mol. Sci. 2022, 23, 11755. https://doi.org/10.3390/ijms231911755
Shi F, Chen X, Wang Y, Xie Y, Zhong J, Su K, Li M, Li Y, Lin Q, Zhou Y, et al. HOTAIR/miR-203/CAV1 Crosstalk Influences Proliferation, Migration, and Invasion in the Breast Cancer Cell. International Journal of Molecular Sciences. 2022; 23(19):11755. https://doi.org/10.3390/ijms231911755
Chicago/Turabian StyleShi, Fuxiu, Xinyue Chen, Yi Wang, Yujie Xie, Junpei Zhong, Kangtai Su, Miao Li, Yuqiu Li, Qing Lin, Youjia Zhou, and et al. 2022. "HOTAIR/miR-203/CAV1 Crosstalk Influences Proliferation, Migration, and Invasion in the Breast Cancer Cell" International Journal of Molecular Sciences 23, no. 19: 11755. https://doi.org/10.3390/ijms231911755