Bioinformatics and Experimental Analyses Reveal Immune-Related LncRNA–mRNA Pair AC011483.1-CCR7 as a Biomarker and Therapeutic Target for Ischemic Cardiomyopathy
Abstract
:1. Introduction
2. Results
2.1. Data Preprocessing and DEGs Screening
2.2. Construction of Weighted Co-Expression Network and Identification of ICM-Related Key Modules
2.3. Identification of Immune-Related LncRNAs in the Key Modules
2.4. The ceRNA Network Construction
2.5. Validation of the Immune-Related lncRNA–mRNA Pairs
2.6. Immune Cell Infiltration
3. Discussion
4. Materials and Methods
4.1. Data Selection
4.2. Data Preprocessing and DEGs Screening
4.3. Co-Expression Network Analysis
4.4. Identification of Immune-Related LncRNAs
4.5. Construction and Analysis of an Immune-Related LncRNA-Associated Competing Endogenous RNA Network
4.6. Construction of Ischemic Cell Model and Cell Culture
4.7. Quantitative Real-Time PCR (qRT-PCR)
4.8. CIBERSORTx
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Immune-Related mRNA | Immune-Related lncRNA | Correlation Coefficient |
---|---|---|
KL | C3orf35 | 0.938991 |
HSPA2 | CCDC144NL-AS1 | −0.92054 |
IL17RB | FP700111.1 | 0.908619 |
IL17RB | AL137230.2 | 0.904658 |
HSPA2 | AL137230.2 | 0.900697 |
HSPA2 | NBR2 | 0.898993 |
OAS1 | AL355483.1 | 0.893618 |
IL17RB | AL353576.1 | 0.891683 |
CD8A | AL109924.2 | −0.89132 |
SSTR2 | NUTM2B-AS1 | 0.891205 |
HSPA2 | AC018462.1 | −0.88867 |
HSPA2 | FP700111.1 | 0.888298 |
SSTR2 | AL357033.1 | −0.88791 |
PPBP | AL355483.1 | 0.886973 |
PPBP | MYCL-AS1 | 0.886181 |
JAG2 | AL445253.1 | 0.886144 |
HSPA2 | AC006115.2 | −0.88587 |
WFIKKN1 | AC099811.3 | 0.885222 |
HSPA2 | AL139011.1 | −0.88446 |
HSPA2 | AC005776.2 | −0.884 |
SORT1 | AC135050.6 | −0.88333 |
IL17RB | LINC00958 | 0.88294 |
HSPA2 | HCG25 | −0.87913 |
CD8A | NBR2 | 0.879123 |
HSPA2 | AL357033.1 | −0.87882 |
CD8A | AL109924.4 | −0.87795 |
HSPA2 | LIPE-AS1 | −0.87617 |
HSPA2 | AC100802.1 | −0.87556 |
IL24 | NBR2 | −0.87349 |
HSPA2 | AP002336.2 | −0.87336 |
SORT1 | LINC01788 | 0.870983 |
CD8A | AL136419.2 | 0.869864 |
HSPA2 | STAM-AS1 | 0.869411 |
HSPA2 | GLYCTK-AS1 | −0.86937 |
CD8A | AL139011.1 | −0.86919 |
SSTR2 | AC018462.1 | −0.86902 |
HSPA2 | Z84492.1 | −0.86834 |
HSPA2 | AC016727.1 | 0.86786 |
HSPA2 | AC008124.1 | 0.867474 |
IL24 | AL590666.2 | 0.867407 |
A2M | AC117945.1 | −0.86682 |
CD8A | CCDC144NL-AS1 | −0.86612 |
PPBP | KIF9-AS1 | 0.865588 |
PPBP | AC015687.1 | 0.865373 |
SORT1 | AL513285.1 | 0.864497 |
PPBP | AC023282.1 | 0.864312 |
HSPA2 | AL445253.1 | −0.86361 |
HSPA2 | SEMA3F-AS1 | −0.86289 |
A2M | AP003721.2 | −0.8627 |
HSPA2 | AC021092.1 | −0.86154 |
Immune-Related mRNA | Immune-Related lncRNA | Correlation Coefficient |
---|---|---|
HSPA2 | NBR2 | 0.898993 |
CD8A | NBR2 | 0.879123 |
NFYC | C3orf35 | 0.853143 |
ITK | C3orf35 | 0.828428 |
PTGFR | C3orf35 | 0.801013 |
KL | C3orf35 | 0.938991 |
SORT1 | C3orf35 | 0.822644 |
PDK1 | C3orf35 | 0.800123 |
IL17RB | LINC00452 | 0.801915 |
HSPA2 | LINC00452 | 0.804171 |
GZMB | UBE2Q1-AS1 | 0.819774 |
HLA-DOB | UBE2Q1-AS1 | 0.807795 |
CCR7 | AC011483.1 | 0.809768 |
MICB | AC011483.1 | 0.812744 |
HSPA2 | AC011407.1 | 0.818169 |
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Jin, Q.; Gong, Q.; Le, X.; He, J.; Zhuang, L. Bioinformatics and Experimental Analyses Reveal Immune-Related LncRNA–mRNA Pair AC011483.1-CCR7 as a Biomarker and Therapeutic Target for Ischemic Cardiomyopathy. Int. J. Mol. Sci. 2022, 23, 11994. https://doi.org/10.3390/ijms231911994
Jin Q, Gong Q, Le X, He J, Zhuang L. Bioinformatics and Experimental Analyses Reveal Immune-Related LncRNA–mRNA Pair AC011483.1-CCR7 as a Biomarker and Therapeutic Target for Ischemic Cardiomyopathy. International Journal of Molecular Sciences. 2022; 23(19):11994. https://doi.org/10.3390/ijms231911994
Chicago/Turabian StyleJin, Qiao, Qian Gong, Xuan Le, Jin He, and Lenan Zhuang. 2022. "Bioinformatics and Experimental Analyses Reveal Immune-Related LncRNA–mRNA Pair AC011483.1-CCR7 as a Biomarker and Therapeutic Target for Ischemic Cardiomyopathy" International Journal of Molecular Sciences 23, no. 19: 11994. https://doi.org/10.3390/ijms231911994