Genome-Wide Identification and Characterization of the RCI2 Gene Family in Allotetraploid Brassica napus Compared with Its Diploid Progenitors
Abstract
:1. Introduction
2. Results
2.1. Identification and Phylogenetic Analysis of RCI2 Gene Family Members
2.2. Chromosomal Localization of the RCI2 Genes
2.3. Synteny and Duplicated Gene Analysis
2.4. The Gene Duplication Types of RCI2 Genes
2.5. Structural Analysis of RCI2 Genes
2.6. Sequence Alignment of All Identified RCI2 Proteins
2.7. Subcellular Localization Analysis of RCI2 Proteins
2.8. Analysis of Cis-Acting Elements in the Promoters of RCI2 Genes
2.9. Analysis of RCI2 Gene Expression
3. Discussion
3.1. The BnRCI2 Gene Family Was Amplified in Allotetraploid
3.2. The Loss of RCI2 Genes in Diploid Progenitors was Associated with the Process of Diploidization after the WGT Event
4. Materials and Methods
4.1. Materials and Transcriptome Sequencing
4.2. Identification of RCI2 Gene Family
4.3. Chromosomal Mapping, Gene Structures and Gene Duplication Types of the RCI2 Gene Family
4.4. Conserved Motif and Characteristic Analysis and Subcellular Localization Analysis
4.5. Phylogenetic Relationship Analysis
4.6. Gene Duplication and Syntenic Analysis
4.7. Cis-Elements Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Replication Types | Number | ||
---|---|---|---|
B. rapa | B. oleracea | B. napus | |
WGD | 9 | 5 | 13 |
TRD | 9 | 5 | 14 |
TD | 9 | 6 | 10 |
PD | 9 | 5 | 7 |
DSD | 8 | 5 | 21 |
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Sun, W.; Li, M.; Wang, J. Genome-Wide Identification and Characterization of the RCI2 Gene Family in Allotetraploid Brassica napus Compared with Its Diploid Progenitors. Int. J. Mol. Sci. 2022, 23, 614. https://doi.org/10.3390/ijms23020614
Sun W, Li M, Wang J. Genome-Wide Identification and Characterization of the RCI2 Gene Family in Allotetraploid Brassica napus Compared with Its Diploid Progenitors. International Journal of Molecular Sciences. 2022; 23(2):614. https://doi.org/10.3390/ijms23020614
Chicago/Turabian StyleSun, Weiqi, Mengdi Li, and Jianbo Wang. 2022. "Genome-Wide Identification and Characterization of the RCI2 Gene Family in Allotetraploid Brassica napus Compared with Its Diploid Progenitors" International Journal of Molecular Sciences 23, no. 2: 614. https://doi.org/10.3390/ijms23020614