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Volume 23
Issue 2
10.3390/ijms23020661
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Article
Peer-Review Record

Glucocorticoid Regulates the Synthesis of Porcine Muscle Protein through m6A Modified Amino Acid Transporter SLC7A7

Int. J. Mol. Sci. 2022, 23(2), 661; https://doi.org/10.3390/ijms23020661
by Wei-Jing Xu 1,2,†, Kai Guo 1,2,†, Jia-Li Shi 1,2, Chang-Tong Guo 1,2, Jia-Le Xu 1,2, Rong Zheng 1,2, Si-Wen Jiang 1,2 and Jin Chai 1,2,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2022, 23(2), 661; https://doi.org/10.3390/ijms23020661
Submission received: 6 December 2021 / Revised: 30 December 2021 / Accepted: 31 December 2021 / Published: 10 January 2022
(This article belongs to the Section Molecular Endocrinology and Metabolism)

Round 1

Reviewer 1 Report

The data included in this manuscript are compelling overall. However I have several concerns that need to be addressed regarding the details and content, which I have outlined below. 

Major concerns:

  • Please quantify puromycin protein synthesis (SUnSET assay) and include in figures.
  • Methodological details are missing or need clarification
    • Species of pig used
    • There is nothing in the methods regarding the cortisol-fed pig experiment shown in Figure 3A. Please include concentration and duration of cortisol, age of pigs, etc.. 
    • MeRIP-qPCR not included in methods--need details of experiment
    • Need to include final concentration of siRNA used, not volume 
  • Substantial grammatical edits are needed throughout.
    • In addition, please attend to the following:
      • Please reduce repetitive statements throughout.
      • Intro rationale and flow needs work- please improve clarification for the reader as to why you are looking into m6A/methylation genes.
  • Please discuss how closely your treatment paradigm compares to that of endogenous circulating cortisol due to stress in pigs.
  • There are a few statements throughout that do not have any citations, please include. 

Minor concerns:

  • Nomenclature of genes and proteins should be consistent throughout
  • Data interpretation is included in the results section 
  • In line 228, you sate that 'our study found that puromycin binding efficiency...treatment of DEX on C2C12 cells' I did not see anything about using C2C12s in the methods or figures. Please clarify if this was a previous study, if so please cite that study (the citations included are not from your group), if not please provide the details of this experiment or correct any mistakes.

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report

GLUCOCORTICOID REGULATES THE SYNTHESIS OF PORCINE MUSCLE PROTEIN THROUGH M6A MODIFIED AMINO ACID RANSPORTER SLC7A7

 

We-Jing Xu and colleagues studied the effect of dexamethasone on the protein synthesis level of porcine skeletal muscle cells.

Since glucocorticoid-induced leucize zipper (gilz) is an early glucococrticoid induced gene, i would ask the authors to see if gilz i salso involved in the patways they studied.

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Round 2

Reviewer 1 Report

I appreciate the authors addressing all of my concerns in detail and revising the manuscript as suggested.

Author Response

Please see the attachment

Author Response File: Author Response.docx

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