Effects of the Chloroplast Fructose-1,6-Bisphosphate Aldolase Gene on Growth and Low-Temperature Tolerance of Tomato
Abstract
:1. Introduction
2. Results
2.1. Production and Selection of Tomato Transformants
2.2. Effects of SlFBA4 on Transgenic Tomato Plant Growth
2.3. Effects of SlFBA4 on Net Photosynthetic Rate as well as Gene Expression and Activity of Select Enzymes in the Calvin-Benson Cycle
2.4. Effects of SlFBA4 on Germination Rate, Net Photosynthetic Rate and Malonaldehyde Content under Chilling Stress
3. Discussion
4. Materials and Methods
4.1. Plant Materials, Growth Conditions and Measurements
4.2. Gene Cloning, Vector Construction and Generation of Transgenic Lines
4.3. Quantitative Reverse Transcription Polymerase Chain Reaction Analysis
4.4. Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and Western Blot Analysis
4.5. Measurement of Net Photosythetic Rate
4.6. Enzyme Activity Assay
4.7. Measurement of Malonaldehyde Content
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
CBC | Calvin-Benson cycle |
CDS | Coding sequence |
cFBA | Cytosolic fructose-1,6-bisphosphate aldolase |
CpFBA | Chloroplast/plastid fructose-1,6-bisphosphate aldolase |
DHAP | Dihydroxyacetone phosphate |
FBA | Fructose-1,6-bisphosphate aldolase |
FBP | Fructose-1,6-bisphosphate |
FBPase | Fructose-1,6-bisphosphatase |
G3P | Glyceraldehyde-3-phosphate |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase |
MDA | Malonaldehyde |
PCR | Polymerase chain reaction |
Pn | Net photosynthetic rate |
qRT-PCR | Quantitative reverse transcription polymerase chain reaction |
RNAi | RNA interference |
Rubisco | Ribulose-1,5-bisphosphate carboxylase/oxygenase |
RuBP | Ribulose-1,5-bisphosphate |
RuBPCase | RuBP carboxylase |
SBPase | Sedoheptulose-1,7-bisphosphatase |
SDS-PAGE | Sodium dodecyl sulfate polyacrylamide gel electrophoresis |
TK | Transketolase |
WT | Wild-type |
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Lines | Thousand-Seed-Weight | Germination Rate (%) | Plant Height (cm) | Stem Diameter (mm) | |
---|---|---|---|---|---|
18 °C | 28 °C | ||||
WT | 2.2 ± 0.3 d | 37.3 ± 5 b | 97.0 ± 4 a | 25.4 ± 2 d | 14.2 ± 0.7 d |
T2-19 | 2.7 ± 0.2 c | 41.0 ± 3 ab | 96.6 ± 4 a | 29.0 ± 1 c | 16.5 ± 0.5 c |
T2-22 | 3.0 ± 0.2 b | 46.0 ± 5 a | 97.0 ± 3 a | 32.2 ± 1 b | 17.2 ± 0.3 b |
T2-33 | 3.2 ± 0.3 a | 48.6 ± 2 a | 99.3 ± 1 a | 35.4 ± 1 a | 21.0 ± 0.6 a |
T2′-16 | 2.1 ± 0.1 e | 34.3 ± 5 bc | 60.0 ± 2 b | 25.8 ± 1 d | 14.1 ± 0.7 d |
T2′-30 | 1.9 ± 0.2 f | 29.0 ± 1 cd | 53.7 ± 5 c | 26 ± 1 d | 13.7 ± 1.1 d |
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Cai, B.; Ning, Y.; Li, Q.; Li, Q.; Ai, X. Effects of the Chloroplast Fructose-1,6-Bisphosphate Aldolase Gene on Growth and Low-Temperature Tolerance of Tomato. Int. J. Mol. Sci. 2022, 23, 728. https://doi.org/10.3390/ijms23020728
Cai B, Ning Y, Li Q, Li Q, Ai X. Effects of the Chloroplast Fructose-1,6-Bisphosphate Aldolase Gene on Growth and Low-Temperature Tolerance of Tomato. International Journal of Molecular Sciences. 2022; 23(2):728. https://doi.org/10.3390/ijms23020728
Chicago/Turabian StyleCai, Bingbing, Yu Ning, Qiang Li, Qingyun Li, and Xizhen Ai. 2022. "Effects of the Chloroplast Fructose-1,6-Bisphosphate Aldolase Gene on Growth and Low-Temperature Tolerance of Tomato" International Journal of Molecular Sciences 23, no. 2: 728. https://doi.org/10.3390/ijms23020728