Lipocalin 2 Influences Bone and Muscle Phenotype in the MDX Mouse Model of Duchenne Muscular Dystrophy
Abstract
:1. Introduction
2. Results
2.1. Lcn2 Levels Are Increased in MDX Mice and Correlate with Il1b and Tnfa Levels
2.2. Genetic Ablation of Lcn2 Improves the Bone Phenotype of MDX Mice
2.3. Genetic Ablation of Lcn2 Improves the Muscle Phenotype of MDX Mice
2.4. Lcn2 Blockade via a Monoclonal Antibody Improves Bone and Muscle Phenotype of MDX Mice
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Study Approval
4.3. Animals
4.4. Forelimb Grip Strenght Tests
4.5. Anti-Lcn2 Antibody Treatment
4.6. Comparative Real-Time RT-PCR
4.7. Histology and Histopathology
4.8. Bone Histomorphometry
4.9. Micro Computed Tomography (µCT) Analysis
4.10. Biodent® Mechanical Testing
4.11. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Ponzetti, M.; Ucci, A.; Maurizi, A.; Giacchi, L.; Teti, A.; Rucci, N. Lipocalin 2 Influences Bone and Muscle Phenotype in the MDX Mouse Model of Duchenne Muscular Dystrophy. Int. J. Mol. Sci. 2022, 23, 958. https://doi.org/10.3390/ijms23020958
Ponzetti M, Ucci A, Maurizi A, Giacchi L, Teti A, Rucci N. Lipocalin 2 Influences Bone and Muscle Phenotype in the MDX Mouse Model of Duchenne Muscular Dystrophy. International Journal of Molecular Sciences. 2022; 23(2):958. https://doi.org/10.3390/ijms23020958
Chicago/Turabian StylePonzetti, Marco, Argia Ucci, Antonio Maurizi, Luca Giacchi, Anna Teti, and Nadia Rucci. 2022. "Lipocalin 2 Influences Bone and Muscle Phenotype in the MDX Mouse Model of Duchenne Muscular Dystrophy" International Journal of Molecular Sciences 23, no. 2: 958. https://doi.org/10.3390/ijms23020958