Iron Deficiency Promotes the Lack of Photosynthetic Cytochrome c550 and Affects the Binding of the Luminal Extrinsic Subunits to Photosystem II in the Diatom Phaeodactylum tricornutum
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Cell Growth
4.2. Protein Analysis Methods
4.3. Photosynthetic Measurements
4.4. Statistical Significance Level
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameter (after 15 Days of Growth) | 10 µM Fe | 0.1 µM Fe | 0.04 µM Fe |
---|---|---|---|
Specific growth rate (µ, day−1) | 0.252 ± 0.002 (100%) | 0.204 ± 0.009 (80.9%) | 0.190 ± 0.011 (75.4%) |
Net respiration rate per 106 cells (nmol O2 h−1) | −0.57 ± 0.02 (100%) | −0.93 ± 0.01 (163.1%) | −0.82 ± 0.01 (142.6%) |
Net photosynthetic rate per 106 cells (nmol O2 h−1) | 6.51 ± 0.03 (100%) | 3.16 ± 0.05 (48.5%) | 2.72 ± 0.04 (41.5%) |
TL intensity (a.u.) | 340.9 ± 20.5 (100%) | 206.7 ± 17.3 (60.6%) | 161.2 ± 9.4 (47.3%) |
Relative PSII content (Fm − F0) | 0.142 ± 0.037 (100%) | 0.084 ± 0.017 (59.1%) | 0.079 ± 0.021 (55.4%) |
Maximum quantum yield of PSII (Fv/Fm) | 0.621 ± 0.016 (100%) | 0.463 ± 0.050 (74.5%) | 0.394 ± 0.014 (63.4%) |
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Castell, C.; Díaz-Santos, E.; Heredia-Martínez, L.G.; López-Maury, L.; Ortega, J.M.; Navarro, J.A.; Roncel, M.; Hervás, M. Iron Deficiency Promotes the Lack of Photosynthetic Cytochrome c550 and Affects the Binding of the Luminal Extrinsic Subunits to Photosystem II in the Diatom Phaeodactylum tricornutum. Int. J. Mol. Sci. 2022, 23, 12138. https://doi.org/10.3390/ijms232012138
Castell C, Díaz-Santos E, Heredia-Martínez LG, López-Maury L, Ortega JM, Navarro JA, Roncel M, Hervás M. Iron Deficiency Promotes the Lack of Photosynthetic Cytochrome c550 and Affects the Binding of the Luminal Extrinsic Subunits to Photosystem II in the Diatom Phaeodactylum tricornutum. International Journal of Molecular Sciences. 2022; 23(20):12138. https://doi.org/10.3390/ijms232012138
Chicago/Turabian StyleCastell, Carmen, Encarnación Díaz-Santos, Luis G. Heredia-Martínez, Luis López-Maury, José M. Ortega, José A. Navarro, Mercedes Roncel, and Manuel Hervás. 2022. "Iron Deficiency Promotes the Lack of Photosynthetic Cytochrome c550 and Affects the Binding of the Luminal Extrinsic Subunits to Photosystem II in the Diatom Phaeodactylum tricornutum" International Journal of Molecular Sciences 23, no. 20: 12138. https://doi.org/10.3390/ijms232012138