Genome-Wide Association and Expression Analysis of the Lipoxygenase Gene Family in Passiflora edulis Revealing PeLOX4 Might Be Involved in Fruit Ripeness and Ester Formation
Abstract
:1. Introduction
2. Results and Analysis
2.1. Identification of LOX Gene Family Members
2.2. Phylogenetic Analysis
2.3. Analysis of PeLOX Gene Family Structure
2.4. Cis-Acting Element Prediction
2.5. Expression Profile Analysis of PeLOX Genes
2.5.1. Transcriptome Analysis under Different Abiotic Stresses
2.5.2. Differential Expression in Seven Tissues
2.5.3. Transcriptome Analysis (qRT-PCR) at Three Different Fruit Development Stages
2.6. Correlation Analysis of PeLOX4 Gene Expression, Enzyme Activity, and Fruit Development
3. Discussion
4. Materials and Methods
4.1. Identification of LOX Gene Family Members in Passion Fruit
4.2. Phylogenetic, Gene Structure and Conserved Motifs Analysis
4.3. Cis-Acting Elements Prediction
4.4. Expression Profile Analysis and Plant Growth Conditions
4.5. qRT-PCR Verification
4.6. Correlation Analysis of PeLOX4 Gene Expression, Enzyme Activity and Fruit Ripeness
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene ID | Gene Location | CDS Length (bp) | Protein Length (aa) | Molecular Formula | Theoretical pI | Subcellular Location | Secondary Structure | ||
---|---|---|---|---|---|---|---|---|---|
Alpha Helix/% | Extended Strand/% | Random Coil/% | |||||||
PeLOX1 | P_edulis010002705.g | 2688 | 895 | C4581H7094N1230O1356S24 | 5.42 | Cytoplasm | 33.10 | 5.05 | 61.85 |
PeLOX2 | P_edulis030007751.g | 1587 | 528 | C2743H4284N730O792S14 | 5.98 | Cytoplasm | 46.40 | 1.70 | 51.89 |
PeLOX3 | P_edulis030007753.g | 2610 | 869 | C4400H6816N1174O1321S20 | 5.51 | Nucleus | 37.77 | 3.27 | 62.72 |
PeLOX4 | P_edulis030007765.g | 2610 | 869 | C4503H6977N1187O1314S19 | 5.55 | Cytoplasm | 30.61 | 6.67 | 62.72 |
PeLOX5 | P_edulis050012485.g | 2697 | 898 | C4631H7151N1239O1341S21 | 5.9 | Chloroplast | 32.63 | 5.26 | 62.11 |
PeLOX6 | P_edulis050012486.g | 2802 | 933 | C4822H7451N1297O1384S22 | 6.27 | Chloroplast | 30.12 | 6.54 | 63.34 |
PeLOX7 | P_edulis050012489.g | 2679 | 892 | C4597H7088N1218O1321S19 | 6.26 | Chloroplast | 33.97 | 4.37 | 61.66 |
PeLOX8 | P_edulis060014414.g | 2649 | 882 | C4500H7048N1270O1301S26 | 9.16 | Nucleus | 31.63 | 8.39 | 59.98 |
PeLOX9 | P_edulis060014719.g | 1419 | 472 | C2443H3770N660O694S12 | 6.5 | Chloroplast | 43.64 | 1.69 | 54.66 |
PeLOX10 | P_edulis060016092.g | 2601 | 866 | C4481H6950N1208O1300S28 | 5.97 | Cytoplasm | 30.72 | 4.97 | 64.32 |
PeLOX11 | P_edulis070018494.g | 2643 | 880 | C4463H6996N1242O1308S22 | 7.16 | Chloroplast | 33.89 | 5.73 | 60.38 |
PeLOX12 | P_edulis090020726.g | 2649 | 882 | C4475H7021N1233O1305S21 | 6.93 | Cytoplasm | 37.59 | 4.01 | 58.40 |
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Huang, D.; Ma, F.; Wu, B.; Lv, W.; Xu, Y.; Xing, W.; Chen, D.; Xu, B.; Song, S. Genome-Wide Association and Expression Analysis of the Lipoxygenase Gene Family in Passiflora edulis Revealing PeLOX4 Might Be Involved in Fruit Ripeness and Ester Formation. Int. J. Mol. Sci. 2022, 23, 12496. https://doi.org/10.3390/ijms232012496
Huang D, Ma F, Wu B, Lv W, Xu Y, Xing W, Chen D, Xu B, Song S. Genome-Wide Association and Expression Analysis of the Lipoxygenase Gene Family in Passiflora edulis Revealing PeLOX4 Might Be Involved in Fruit Ripeness and Ester Formation. International Journal of Molecular Sciences. 2022; 23(20):12496. https://doi.org/10.3390/ijms232012496
Chicago/Turabian StyleHuang, Dongmei, Funing Ma, Bin Wu, Wenhui Lv, Yi Xu, Wenting Xing, Di Chen, Bingqiang Xu, and Shun Song. 2022. "Genome-Wide Association and Expression Analysis of the Lipoxygenase Gene Family in Passiflora edulis Revealing PeLOX4 Might Be Involved in Fruit Ripeness and Ester Formation" International Journal of Molecular Sciences 23, no. 20: 12496. https://doi.org/10.3390/ijms232012496