Lipid Droplets in Lung Cancers Are Crucial for the Cell Growth and Starvation Survival
Abstract
:1. Introduction
2. Results
2.1. Expression of Molecules Involved in Lipid Biosynthesis and Uptake in Lung Cancer Cells
2.2. Expression of Transcriptional Regulators Related to Lipid Biosynthesis
2.3. Lipid Droplets in Lung Cancer Cells
2.4. Lipid Droplet Formation Induced in Lung Cancer Cells under Lipoprotein-Deficient Growth Medium
2.5. Blocking of Lipid Drops’ Formation Slows down the Cell Proliferation of the Lung Cancer Cells
2.6. Lipid Droplets Increase Survival of Lung Cancer Cells under Starvation
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Oil Red O Staining and Quantitation
4.3. Annexin V/propidium Iodide (PI) Staining Assay
4.4. Immunoblot Analysis
4.5. Chemicals and Antibodies
4.6. Immunohistochemistry
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lung, J.; Hung, M.-S.; Wang, T.-Y.; Chen, K.-L.; Luo, C.-W.; Jiang, Y.-Y.; Wu, S.-Y.; Lee, L.-W.; Lin, P.-Y.; Chen, F.-F.; et al. Lipid Droplets in Lung Cancers Are Crucial for the Cell Growth and Starvation Survival. Int. J. Mol. Sci. 2022, 23, 12533. https://doi.org/10.3390/ijms232012533
Lung J, Hung M-S, Wang T-Y, Chen K-L, Luo C-W, Jiang Y-Y, Wu S-Y, Lee L-W, Lin P-Y, Chen F-F, et al. Lipid Droplets in Lung Cancers Are Crucial for the Cell Growth and Starvation Survival. International Journal of Molecular Sciences. 2022; 23(20):12533. https://doi.org/10.3390/ijms232012533
Chicago/Turabian StyleLung, Jrhau, Ming-Szu Hung, Ting-Yao Wang, Kuan-Liang Chen, Chi-Wen Luo, Yuan-Yuan Jiang, Shin-Yi Wu, Li-Wen Lee, Paul-Yann Lin, Fen-Fen Chen, and et al. 2022. "Lipid Droplets in Lung Cancers Are Crucial for the Cell Growth and Starvation Survival" International Journal of Molecular Sciences 23, no. 20: 12533. https://doi.org/10.3390/ijms232012533