Differences in Leaf Morphology and Related Gene Expression between Diploid and Tetraploid Birch (Betula pendula)
Abstract
:1. Introduction
2. Results
2.1. Differences in Leaf Morphology between Diploid and Tetraploid Birch
2.2. No Change in the Polarity Pattern of Leaf Growth in Tetraploid Birch
2.3. The Leaf Area of Tetraploid Birch Is Significantly Larger Than That of Diploid
2.4. Cell Expansion Causes the Large Size of Tetraploid Birch Leaves
2.5. Gene Expression of the ATHB12 Is Significantly Correlated with Leaf Size in Birch
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Determination of Ploidy Levels in Birch
4.3. Measurement of Leaf Dimensional Blade Parameters
4.4. Leaf Morphometric Analysis
4.5. Leaf Growth Analysis
4.6. Determination of the Size and Number of Leaf Cells
4.7. Measurement of Leaf Growth Ratio (α)
4.8. qRT-PCR Analysis
4.9. Pearson Correlation Analysis
4.10. RNA-Seq Experiment and Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhang, X.; Chen, K.; Wang, W.; Liu, G.; Yang, C.; Jiang, J. Differences in Leaf Morphology and Related Gene Expression between Diploid and Tetraploid Birch (Betula pendula). Int. J. Mol. Sci. 2022, 23, 12966. https://doi.org/10.3390/ijms232112966
Zhang X, Chen K, Wang W, Liu G, Yang C, Jiang J. Differences in Leaf Morphology and Related Gene Expression between Diploid and Tetraploid Birch (Betula pendula). International Journal of Molecular Sciences. 2022; 23(21):12966. https://doi.org/10.3390/ijms232112966
Chicago/Turabian StyleZhang, Xiaoyue, Kun Chen, Wei Wang, Guifeng Liu, Chuanping Yang, and Jing Jiang. 2022. "Differences in Leaf Morphology and Related Gene Expression between Diploid and Tetraploid Birch (Betula pendula)" International Journal of Molecular Sciences 23, no. 21: 12966. https://doi.org/10.3390/ijms232112966