Characterization of Glossy Spike Mutants and Identification of Candidate Genes Regulating Cuticular Wax Synthesis in Barley (Hordeum vulgare L.)
Abstract
:1. Introduction
2. Results
2.1. Phenotypic and Physiological Analysis of the Spike Cuticular Wax-Deficient Cer-GN1 Mutant
2.2. Genetic Analysis of the Spike Cuticular Wax-Deficient Cer-GN1 Mutant
2.3. Identification of Differential Metabolites in Spike Glumes between Cer-GN1 and WT
2.4. Global Analysis of DEGs in Cer-GN1 and WT Spike Glumes
2.5. Cer-GN1 DEGs Are Associated with Wax Biosynthesis
2.6. Verification of HvMSTRG.29184 and HvMSTRG.29185 Gene Expression by qRT-PCR
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Spike Sampling and Analysis
4.3. Metabolite Extraction, Detection, and Analysis
4.4. RNA Extraction, Library Construction, and Sequencing
4.5. Bioinformatics Analysis
4.6. Quantitative RT-PCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Crossing Combination | F1 Plants | F2 Populations | ||||
---|---|---|---|---|---|---|
Total Plants | Glaucous Plants | Glossy Plants | χ2 3:1 | χ2 0.05 | ||
Cer-GN1 × Mei 42 | Glaucous | 488 | 372 | 116 | 0.3307 | 3.84 |
Cer-GN1 × Ganpi 6 | Glaucous | 552 | 417 | 129 | 0.0604 | 3.84 |
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Bian, X.; Yao, L.; Si, E.; Meng, Y.; Li, B.; Ma, X.; Yang, K.; Lai, Y.; Shang, X.; Li, C.; et al. Characterization of Glossy Spike Mutants and Identification of Candidate Genes Regulating Cuticular Wax Synthesis in Barley (Hordeum vulgare L.). Int. J. Mol. Sci. 2022, 23, 13025. https://doi.org/10.3390/ijms232113025
Bian X, Yao L, Si E, Meng Y, Li B, Ma X, Yang K, Lai Y, Shang X, Li C, et al. Characterization of Glossy Spike Mutants and Identification of Candidate Genes Regulating Cuticular Wax Synthesis in Barley (Hordeum vulgare L.). International Journal of Molecular Sciences. 2022; 23(21):13025. https://doi.org/10.3390/ijms232113025
Chicago/Turabian StyleBian, Xiuxiu, Lirong Yao, Erjing Si, Yaxiong Meng, Baochun Li, Xiaole Ma, Ke Yang, Yong Lai, Xunwu Shang, Chengdao Li, and et al. 2022. "Characterization of Glossy Spike Mutants and Identification of Candidate Genes Regulating Cuticular Wax Synthesis in Barley (Hordeum vulgare L.)" International Journal of Molecular Sciences 23, no. 21: 13025. https://doi.org/10.3390/ijms232113025