Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis
Abstract
:1. Introduction
2. Results
2.1. The Wound Healing Potential of Oleosomes Fused with Human Fibroblast Growth Factor 1 in Rats
2.2. The Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on the Collagen Deposition and CD68+ Macrophage Infiltration of Wounds
2.3. Oleosomes Fused with Human Fibroblast Growth Factor 1 Facilitated Angiogenesis at the Wound Sites
2.4. The Effect of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Vascular Permeability in Rats
2.5. The Oleosomes Fused with Human Fibroblast Growth Factor 1 Stimulated Angiogenesis on CAM
2.6. OLAF-Induced Cell Proliferation, Scratch Wound Healing, Migration and Tube Formation in HUVECs
2.7. OLAF Improved Angiogenesis through the PI3K-AKT Pathway
3. Discussion
4. Methods and Materials
4.1. Oleosome Extraction Procedure
4.2. Wound Healing Potential of Oleosomes Fused with hFGF1 in Full-Thickness Skin Defect Rats
4.2.1. Creation and Treatment of Full-Thickness Skin Defect Rat Models
4.2.2. Monitoring of Wound Closure and Specimen Collection
4.2.3. Histopathological, Immunohistochemical and Immunofluorescence Assessment
4.2.4. Evan’s Blue Assay
4.3. Chick Embryo Chorioallantoic Membrane (CAM) Assay for the Pro-Angiogenic Potential of OLAF
4.4. In Vitro Evaluation of the Beneficial Effect of OLAF on Angiogenesis
4.4.1. Cell Culture
4.4.2. Cell Proliferation Vitality Assessment and Morphological Observation
4.4.3. Scratch Wound Assay and Transwell Migration Test
4.4.4. Tube Formation Assay
4.5. RNA-Seq-Based Transcriptome Analysis of HUVECs under OLAF Treatment
4.6. Western Blot and qRT-PCR
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Guo, Y.; Chu, G.; Cai, W.; Li, Y.; Lan, X.; Li, J.; Du, L.; Yang, J. Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis. Int. J. Mol. Sci. 2022, 23, 13152. https://doi.org/10.3390/ijms232113152
Guo Y, Chu G, Cai W, Li Y, Lan X, Li J, Du L, Yang J. Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis. International Journal of Molecular Sciences. 2022; 23(21):13152. https://doi.org/10.3390/ijms232113152
Chicago/Turabian StyleGuo, Yongxin, Guodong Chu, Weijia Cai, Yaying Li, Xinxin Lan, Jing Li, Linna Du, and Jing Yang. 2022. "Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis" International Journal of Molecular Sciences 23, no. 21: 13152. https://doi.org/10.3390/ijms232113152