Overexpression of β-Ketoacyl CoA Synthase 2B.1 from Chenopodium quinoa Promotes Suberin Monomers’ Production and Salt Tolerance in Arabidopsis thaliana

Tariq, Faheem; Zhao, Shuangshuang; Ahmad, Naveed; Wang, Pingping; Shao, Qun; Ma, Changle; Yang, Xianpeng

doi:10.3390/ijms232113204

Open AccessArticle

Overexpression of β-Ketoacyl CoA Synthase 2B.1 from Chenopodium quinoa Promotes Suberin Monomers’ Production and Salt Tolerance in Arabidopsis thaliana

by

Faheem Tariq

^1,†

,

Shuangshuang Zhao

^1,†,

Naveed Ahmad

²

,

Pingping Wang

¹,

Qun Shao

¹,

Changle Ma

^1,* and

Xianpeng Yang

^1,*

¹

Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan 250014, China

²

Institute of Crop and Germplasm Resource (Institute of Biotechnology), Shandong Academy of Agricultural Sciences, Jinan 250100, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2022, 23(21), 13204; https://doi.org/10.3390/ijms232113204

Submission received: 10 September 2022 / Revised: 12 October 2022 / Accepted: 26 October 2022 / Published: 30 October 2022

(This article belongs to the Special Issue Molecular Regulatory Mechanisms of Salinity Tolerance in Plants)

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Abstract

Very-long-chain fatty acids (VLCFAs) are precursors for the synthesis of various lipids, such as triacylglycerols, sphingolipids, cuticular waxes, and suberin monomers, which play important roles in plant growth and stress responses. However, the underlying molecular mechanism regulating VLCFAs’ biosynthesis in quinoa (Chenopodium quinoa Willd.) remains unclear. In this study, we identified and functionally characterized putative 3-ketoacyl-CoA synthases (KCSs) from quinoa. Among these KCS genes, CqKCS2B.1 showed high transcript levels in the root tissues and these were rapidly induced by salt stress. CqKCS2B.1 was localized to the endoplasmic reticulum. Overexpression of CqKCS2B.1 in Arabidopsis resulted in significantly longer primary roots and more lateral roots. Ectopic expression of CqKCS2B.1 in Arabidopsis promoted the accumulation of suberin monomers. The occurrence of VLCFAs with C22–C24 chain lengths in the overexpression lines suggested that CqKCS2B.1 plays an important role in the elongation of VLCFAs from C20 to C24. The transgenic lines of overexpressed CqKCS2B.1 showed increased salt tolerance, as indicated by an increased germination rate and improved plant growth and survival under salt stress. These findings highlight the significant role of CqKCS2B.1 in VLCFAs’ production, thereby regulating suberin biosynthesis and responses to salt stress. CqKCS2B.1 could be utilized as a candidate gene locus to breed superior, stress-tolerant quinoa cultivars.

Keywords: Chenopodium quinoa; β-Ketoacyl CoA Synthase2B.1; very-long-chain fatty acids; suberin monomers; lateral root; salt tolerance

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MDPI and ACS Style

Tariq, F.; Zhao, S.; Ahmad, N.; Wang, P.; Shao, Q.; Ma, C.; Yang, X. Overexpression of β-Ketoacyl CoA Synthase 2B.1 from Chenopodium quinoa Promotes Suberin Monomers’ Production and Salt Tolerance in Arabidopsis thaliana. Int. J. Mol. Sci. 2022, 23, 13204. https://doi.org/10.3390/ijms232113204

AMA Style

Tariq F, Zhao S, Ahmad N, Wang P, Shao Q, Ma C, Yang X. Overexpression of β-Ketoacyl CoA Synthase 2B.1 from Chenopodium quinoa Promotes Suberin Monomers’ Production and Salt Tolerance in Arabidopsis thaliana. International Journal of Molecular Sciences. 2022; 23(21):13204. https://doi.org/10.3390/ijms232113204

Chicago/Turabian Style

Tariq, Faheem, Shuangshuang Zhao, Naveed Ahmad, Pingping Wang, Qun Shao, Changle Ma, and Xianpeng Yang. 2022. "Overexpression of β-Ketoacyl CoA Synthase 2B.1 from Chenopodium quinoa Promotes Suberin Monomers’ Production and Salt Tolerance in Arabidopsis thaliana" International Journal of Molecular Sciences 23, no. 21: 13204. https://doi.org/10.3390/ijms232113204

APA Style

Tariq, F., Zhao, S., Ahmad, N., Wang, P., Shao, Q., Ma, C., & Yang, X. (2022). Overexpression of β-Ketoacyl CoA Synthase 2B.1 from Chenopodium quinoa Promotes Suberin Monomers’ Production and Salt Tolerance in Arabidopsis thaliana. International Journal of Molecular Sciences, 23(21), 13204. https://doi.org/10.3390/ijms232113204

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Overexpression of β-Ketoacyl CoA Synthase 2B.1 from Chenopodium quinoa Promotes Suberin Monomers’ Production and Salt Tolerance in Arabidopsis thaliana

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