Effects of Three Different Doses of Inter-Alpha Inhibitor Proteins on Severe Hypoxia–Ischemia-Related Brain Injury in Neonatal Rats
Abstract
:1. Introduction
2. Results
2.1. Concentrations of hIAIPs in Rat Serum
2.2. Body and Brain Weight
2.3. Behavioral Assessment
2.3.1. Righting Reflex
2.3.2. Negative Geotaxis
2.3.3. Small Open-Field Task
2.4. Brain Infarct Volumes
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Surgeries and Study Design
4.3. Preparation of hIAIPs
4.4. Behavioral Analyses
4.4.1. Righting Reflex
4.4.2. Negative Geotaxis
4.4.3. Small Open-Field Tasks
4.5. Sample Collection
4.6. Histology
4.7. ELISA of hIAIP Concentrations in Rat Serum
4.8. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Koehn, L.M.; Nguyen, K.; Chen, X.; Santoso, A.; Tucker, R.; Lim, Y.-P.; Stonestreet, B.S. Effects of Three Different Doses of Inter-Alpha Inhibitor Proteins on Severe Hypoxia–Ischemia-Related Brain Injury in Neonatal Rats. Int. J. Mol. Sci. 2022, 23, 13473. https://doi.org/10.3390/ijms232113473
Koehn LM, Nguyen K, Chen X, Santoso A, Tucker R, Lim Y-P, Stonestreet BS. Effects of Three Different Doses of Inter-Alpha Inhibitor Proteins on Severe Hypoxia–Ischemia-Related Brain Injury in Neonatal Rats. International Journal of Molecular Sciences. 2022; 23(21):13473. https://doi.org/10.3390/ijms232113473
Chicago/Turabian StyleKoehn, Liam M., Kevin Nguyen, Xiaodi Chen, Andre Santoso, Richard Tucker, Yow-Pin Lim, and Barbara S. Stonestreet. 2022. "Effects of Three Different Doses of Inter-Alpha Inhibitor Proteins on Severe Hypoxia–Ischemia-Related Brain Injury in Neonatal Rats" International Journal of Molecular Sciences 23, no. 21: 13473. https://doi.org/10.3390/ijms232113473