Integrative Metabolomic and Transcriptomic Analysis Reveals the Mechanism of Specific Color Formation in Phoebe zhennan Heartwood
Abstract
:1. Introduction
2. Results
2.1. Comparison of DAMs among Sapwood, the Transition Zone, and Heartwood
2.2. Screening of Key Golden Thread-Related Metabolites in Nanmu Heartwood
2.3. Transcriptome Analysis and DEG Identification in Different Tissues
2.4. Correlation between Metabolites and Genes
2.5. Phenylpropanoid and Flavonoid Biosynthesis Pathways Related to Nanmu Special Color Heartwood Formation
2.6. Transcription Factors Related to Phenylpropanoid and Flavonoid Biosynthesis
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Sampling
4.2. Microscopy Analyses and Flavonoid Content Measurement
4.3. Identification and Quantification of Metabolites
4.4. RNA-Seq Analysis
4.5. Real-Time Quantitative PCR
4.6. Integrative Transcriptomic and Metabolomic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yang, H.; An, W.; Gu, Y.; Peng, J.; Jiang, Y.; Li, J.; Chen, L.; Zhu, P.; He, F.; Zhang, F.; et al. Integrative Metabolomic and Transcriptomic Analysis Reveals the Mechanism of Specific Color Formation in Phoebe zhennan Heartwood. Int. J. Mol. Sci. 2022, 23, 13569. https://doi.org/10.3390/ijms232113569
Yang H, An W, Gu Y, Peng J, Jiang Y, Li J, Chen L, Zhu P, He F, Zhang F, et al. Integrative Metabolomic and Transcriptomic Analysis Reveals the Mechanism of Specific Color Formation in Phoebe zhennan Heartwood. International Journal of Molecular Sciences. 2022; 23(21):13569. https://doi.org/10.3390/ijms232113569
Chicago/Turabian StyleYang, Hanbo, Wenna An, Yunjie Gu, Jian Peng, Yongze Jiang, Jinwu Li, Lianghua Chen, Peng Zhu, Fang He, Fan Zhang, and et al. 2022. "Integrative Metabolomic and Transcriptomic Analysis Reveals the Mechanism of Specific Color Formation in Phoebe zhennan Heartwood" International Journal of Molecular Sciences 23, no. 21: 13569. https://doi.org/10.3390/ijms232113569