Zona Pellucida like Domain Protein 1 (ZPLD1) Polymerization Is Regulated by Two Distinguished Hydrophobic Motifs
Abstract
:1. Introduction
2. Results
2.1. Transformed MDCK Cells Express Physiological Active ZPLD1
2.2. ZPLD1 Proteins Lacking the EHP or IHP Are Not Properly Processed
2.3. Depletion of EHP or IHP Leads to the Formation of Intracellular Polymeric Structures
2.4. Release of the C-Terminal Pro-Peptide and Hence Loss of the Putative EHP Is a Prerequisite for Proper Extracellular Polymerization
3. Discussion
4. Materials and Methods
4.1. Protein Sequence Analyses
4.2. ZPLD1 Expression Constructs
4.3. Cell Lines and Culture Conditions
4.4. Protein Purification and Western Blot
4.5. Immunofluorescence
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Knepper, M.I.; Dernedde, J. Zona Pellucida like Domain Protein 1 (ZPLD1) Polymerization Is Regulated by Two Distinguished Hydrophobic Motifs. Int. J. Mol. Sci. 2022, 23, 13894. https://doi.org/10.3390/ijms232213894
Knepper MI, Dernedde J. Zona Pellucida like Domain Protein 1 (ZPLD1) Polymerization Is Regulated by Two Distinguished Hydrophobic Motifs. International Journal of Molecular Sciences. 2022; 23(22):13894. https://doi.org/10.3390/ijms232213894
Chicago/Turabian StyleKnepper, Marie Isabell, and Jens Dernedde. 2022. "Zona Pellucida like Domain Protein 1 (ZPLD1) Polymerization Is Regulated by Two Distinguished Hydrophobic Motifs" International Journal of Molecular Sciences 23, no. 22: 13894. https://doi.org/10.3390/ijms232213894