In Vivo Preclinical Assessment of β-Amyloid–Affine [11C]C-PIB Accumulation in Aluminium-Induced Alzheimer’s Disease-Resembling Hypercholesterinaemic Rat Model
Abstract
:1. Introduction
2. Results
2.1. Results of Blood Test Analysis
2.2. In Vivo Positron Emission Tomography (PET) Imaging Results after One Month of Combined (Cholesterol and Al2(SO4)3) Treatment
2.3. Results of PET Examinations One and Two Months after Restoration to Normal Diet and Water
3. Discussion
4. Materials and Methods
4.1. Experimental Animals
4.2. Chemical Induction of the Experimental Animals
- Group 1 diet rich in butter and cholesterol and normal tap water ad libitum.
- Group 2 normal rodent chow and drinking water containing 200 mg/L Al2(SO4)3 ad libitum.
- Group 3 diet rich in butter and cholesterol and drinking water containing 200 mg/L Al2(SO4)3 ad libitum.
- Group 4 normal rodent chow and normal tap water ad libitum (control group).
4.3. PET Examinations
4.4. Analyses of Serum Blood Samples
4.5. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
AD | Alzheimer’s disease |
APP | Amyloid Precursor Protein |
BCR | butter cholesterol rich |
CCH | chronic cerebral hypoperfusion |
Aβ plaques | β-amyloid plaques |
[11C]C-PIB | [11C]-labelled Pittsburgh Compound-B |
[18F]F-FDG | [18F]-2-fluoro-deoxy-D-glucose |
HDL | high-density lipoprotein cholesterol |
HFCD | high fat cholesterol diet |
iAβ | intracellular β-amyloid |
i.v. | intravenous |
LDL | low-density lipoprotein cholesterol |
NFTs | neurofibrillary tangles |
PET | positron emission tomography |
SUV | standardised uptake value |
VOI | volume of interest |
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Standard VRF1 Diet | |
---|---|
Component | Composition (%) |
moisture | 10.00 |
crude protein | 19.11 |
crude fat | 4.75 |
crude fibre | 3.85 |
ash | 6.97 |
NFE | 55.32 |
BCR Diet | |
---|---|
Component | Composition (%) |
Standard VRF1 feed | 60.00 |
casein | 9.50 |
butter | 20.00 |
cholesterol | 4.00 |
sodium cholate | 1.00 |
“SM8” salt mixture, according to Sós [49] | 5.00 |
6-Methyl-2-thio-uracil | 0.50 |
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Képes, Z.; Barkóczi, A.; Szabó, J.P.; Kálmán-Szabó, I.; Arató, V.; Jószai, I.; Deák, Á.; Kertész, I.; Hajdu, I.; Trencsényi, G. In Vivo Preclinical Assessment of β-Amyloid–Affine [11C]C-PIB Accumulation in Aluminium-Induced Alzheimer’s Disease-Resembling Hypercholesterinaemic Rat Model. Int. J. Mol. Sci. 2022, 23, 13950. https://doi.org/10.3390/ijms232213950
Képes Z, Barkóczi A, Szabó JP, Kálmán-Szabó I, Arató V, Jószai I, Deák Á, Kertész I, Hajdu I, Trencsényi G. In Vivo Preclinical Assessment of β-Amyloid–Affine [11C]C-PIB Accumulation in Aluminium-Induced Alzheimer’s Disease-Resembling Hypercholesterinaemic Rat Model. International Journal of Molecular Sciences. 2022; 23(22):13950. https://doi.org/10.3390/ijms232213950
Chicago/Turabian StyleKépes, Zita, Alexandra Barkóczi, Judit P. Szabó, Ibolya Kálmán-Szabó, Viktória Arató, István Jószai, Ádám Deák, István Kertész, István Hajdu, and György Trencsényi. 2022. "In Vivo Preclinical Assessment of β-Amyloid–Affine [11C]C-PIB Accumulation in Aluminium-Induced Alzheimer’s Disease-Resembling Hypercholesterinaemic Rat Model" International Journal of Molecular Sciences 23, no. 22: 13950. https://doi.org/10.3390/ijms232213950