Hepatocyte DAX1 Deletion Exacerbates Inflammatory Liver Injury by Inducing the Recruitment of CD4+ and CD8+ T Cells through NF-κB p65 Signaling Pathway in Mice
Abstract
:1. Introduction
2. Results
2.1. Loss of DAX1 in Myeloid Cells Does Not Affect ConA-Induced Liver Injury
2.2. DAX1 Deficiency in Hepatocytes Elevates the Susceptibility to ConA-Induced Liver Injury
2.3. Hepatic Expression Levels of Inflammatory Cytokines Are Increased in Dax1 LKO Mice following ConA Treatment
2.4. Absence of DAX1 in Hepatocytes Enhances CD4+ and CD8+ T-Cell Infiltration against ConA-Induced Liver Injury
2.5. DAX1 Modulates the Expression of Both Chemokines and Adhesion Molecules in Hepatocytes to Promote CD4+ and CD8+ T-Cell Migration
2.6. Hepatocyte DAX1 Deletion Negatively Regulates ConA-Induced Hepatic Injury via Activation of NF-κB p65
3. Discussion
4. Materials and Methods
4.1. Animals Studies
4.2. Blood Analysis
4.3. Histopathology and Immunohistochemistry
4.4. Quantitative Real-Time PCR (RT-qPCR)
4.5. Isolation of Intrahepatic Immune Cells
4.6. Flow-Cytometric Analysis of Intrahepatic Immune Cells
4.7. Bone Marrow-Derived Neutrophils
4.8. Isolation of Primary Mouse Hepatocytes and In Vitro TNFα Treatment
4.9. Western Blot Analysis
4.10. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yun, H.-J.; Suh, Y.-J.; Kim, Y.-B.; Kang, E.-J.; Choi, J.H.; Choi, Y.-K.; Lee, I.-B.; Choi, D.-H.; Seo, Y.J.; Noh, J.-R.; et al. Hepatocyte DAX1 Deletion Exacerbates Inflammatory Liver Injury by Inducing the Recruitment of CD4+ and CD8+ T Cells through NF-κB p65 Signaling Pathway in Mice. Int. J. Mol. Sci. 2022, 23, 14009. https://doi.org/10.3390/ijms232214009
Yun H-J, Suh Y-J, Kim Y-B, Kang E-J, Choi JH, Choi Y-K, Lee I-B, Choi D-H, Seo YJ, Noh J-R, et al. Hepatocyte DAX1 Deletion Exacerbates Inflammatory Liver Injury by Inducing the Recruitment of CD4+ and CD8+ T Cells through NF-κB p65 Signaling Pathway in Mice. International Journal of Molecular Sciences. 2022; 23(22):14009. https://doi.org/10.3390/ijms232214009
Chicago/Turabian StyleYun, Hyo-Jeong, Young-Joo Suh, Yu-Bin Kim, Eun-Jung Kang, Jung Hyeon Choi, Young-Keun Choi, In-Bok Lee, Dong-Hee Choi, Yun Jeong Seo, Jung-Ran Noh, and et al. 2022. "Hepatocyte DAX1 Deletion Exacerbates Inflammatory Liver Injury by Inducing the Recruitment of CD4+ and CD8+ T Cells through NF-κB p65 Signaling Pathway in Mice" International Journal of Molecular Sciences 23, no. 22: 14009. https://doi.org/10.3390/ijms232214009