Next Article in Journal
Properties of Protein Isolates from Marine Hydrobionts Obtained by Isoelectric Solubilisation/Precipitation: Influence of Temperature and Processing Time
Previous Article in Journal
Flavones, Flavonols, Lignans, and Caffeic Acid Derivatives from Dracocephalum moldavica and Their In Vitro Effects on Multiple Myeloma and Acute Myeloid Leukemia
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 23
Issue 22
10.3390/ijms232214213
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

DORN1 Is Involved in Drought Stress Tolerance through a Ca2+-Dependent Pathway

by
Qingwen Wang
1,2,
Hongbao Bai
1,
Ahmad Zada
1 and
Qingsong Jiao
1,3,*
1
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China
2
National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210046, China
3
Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(22), 14213; https://doi.org/10.3390/ijms232214213
Submission received: 18 October 2022 / Revised: 12 November 2022 / Accepted: 15 November 2022 / Published: 17 November 2022
(This article belongs to the Section Molecular Plant Sciences)
Browse Figures
Review Reports Versions Notes

Abstract

:
Water shortages caused by climate change seriously threaten the survival and production of plants and are also one of the major environmental pressures faced by plants. DORN1 was the first identified purinoceptor for the plant response to extracellular ATP. It has been established that DORN1 could play key roles in a series of biological activities in plants. However, the biological roles of DORN1 and the mechanism remain unclear under drought stress conditions in plants. Here, DORN1 was targeted for knockout by using the CRISPR/Cas 9 system. It was found that the loss function of DORN1 resulted in a significant decrease in the effective quantum yield of PSII [Y(II)], the photochemical quenching coefficient (qP), and the rate of photosynthetic electron transport through PSII (ETR), which reflected plants’ photochemical efficiency. Whereas Y(NO) values showed obvious enhancement under drought stress conditions. Further experimental results showed that the Y(II), qP, and ETR, which reflect plants’ photochemical efficiency, increased significantly with CaCl2 treatment. These results indicated that the drought tolerance of the mutant was decreased, and the exogenous application of calcium ions could effectively promote the drought tolerance of the dorn1 mutant. Transpiration loss controlled by stomata is closely related to drought tolerance, further, we examined the transpirational water loss in dorn1 and found that it was greater than wild-type (WT). Besides, the dorn1 mutant’s stomatal aperture significantly increased compared with the WT and the stomata of dorn1 mutant plants tend to close after CaCl2 treatment. Taken together, our results show that DORN1 plays a key role in drought stress tolerance in plants, which may depend on calcium and calcium-related signaling pathways.

1. Introduction

Drought stress is one of the biggest stresses on plant growth and development. Recently, it has become more accentuated due to global warming. Stomata are leaf pores that control gas exchange and water transport, and therefore, impact critical functions such as photosynthesis and drought tolerance. Studies have shown that Ca2+-dependent signaling plays a role in regulating stomatal movement [1], and this Ca2+-regulated stomatal movement is crucial to water use efficiency and drought tolerance in plants [2,3].
Besides being a cellular energy source, the adenosine triphosphate (ATP) can be transferred to the extracellular space through wound leakage, secretion pathways, or membrane transporter. Extracellular ATP (eATP) could act as a famous signal molecule used by plants and functions in various processes, such as growth, development, and stress responses [4,5,6]. These functions depend on the cell surface receptor eATP receptors and activate second messenger molecules intracellularly, such as cytosolic free Ca2+ ([Ca2+]cyt) and hydrogen peroxide [7,8]. As one of the famous second messengers, [Ca2+]cyt plays a vital role in plants’ development and stress tolerance [3,9,10]. Such as brassinosteroid biosynthesis and plant growth [11], balancing plant growth and immunity [12], and enhancing normal pollen tube growth and fertilization [13]. Recently, does not respond to nucleotides (DORN1, also known as P2K1) and has been identified as the first purinoceptor on the surface of the plant cell, which is essential for plant response to ATP [14]. DORN1 is a legume-type lectin receptor kinase that became widely known as an eATP receptor through its role in increasing [Ca2+]cyt in seedlings [14]. Transgenic plants overexpressing DORN1 showed longer roots length, a greater rosette surface area, and more resistance to the pathogen [15,16]. The dorn1 mutant plants had a reduced transcriptional response to both ATP treatment and wounding [17], as well as pathogen resistance [18]. Although there is mounting evidence that DORN1 plays a role in plant biotic stress tolerance, the mechanisms of signal transduction through DORN1 remain largely unknown.
eATP and its receptor DORN1 have been established as an important signaling pathway responding to salt stress tolerance [19], chilling response [20], and biotic stress resistance [21,22] in plants. Drought stress is a major factor limiting plant growth and development in nature, and it is also an important factor affecting crop production. However, little is known about whether DORN1 is involved in drought stress tolerance and the possible mechanisms. As a rapid, noninvasive technique for detecting subtle differences in leaf metabolism or developmental disorders of the seedlings [23]. It has long been known that the chlorophyll fluorescence induction kinetics of leaves from plants provide an indicator of plant physiological state and are sensitive to changes in stress responses [24]. The activity of photosystem II (PSII) declines more rapidly than any other physiological activity [25,26]. In the present study, we created the dorn1 mutant by CRISPR/Cas9 and found that the loss functions of DORN1 impaired drought stress tolerance in plants, which may be related to the Ca2+-regulated stomatal movement. These findings revealed the role and underlying mechanism of DORN1 in maintaining drought tolerance in plants and also offered a strategy for enhancing plant drought tolerance.

2. Results

2.1. Creation and Identification of the Loss Function Mutants for DORN1

CRISPR/Cas9 provided a convenient tool for creating loss-of-function mutants for genes of interest. To create mutations in DORN1, synthetic guide RNA targeting the first exon of DORN1 was designed by the E-CRISP tool. Finally, we successfully achieved site-specific deletion of DORN1, dorn1-c3 with an 8 bp deletion, and dorn1-c6 with a 7 bp insertion and an 8 bp deletion (Figure 1A,B). Phenotypic analyses showed the mutant plants could grow normally under normal conditions, and there was no significant difference in fresh weight analysis (Figure 1C,D).

2.2. The Survival Rate Was Decreased at Day 10 of Soil drying in the dorn1 Mutant

Water shortages are a global environmental threat that seriously threatens plants. To determine whether the DORN1 functions in drought tolerance, we further confirmed this by phenotypic analysis of plants with direct drought treatment. After 10 days of drought treatment, the dorn1 mutant plants mutant showed obvious severe dehydration and could not survive after rehydration (Figure 2A). The survival rate is often taken as an indicator of the drought tolerance of plants. WT and the dorn1 mutant plants were rehydrated after 10 days of drought treatment, and the survival rate was calculated by counting the number of surviving plants at 2 days after rewatering the plants. The ratio of surviving plants to total treated plants was then calculated. Statistical analysis showed that the survival rate of the mutant decreased significantly after rehydration (Figure 2B).

2.3. Loss Function of DORN1 Has No Obvious Influence on Fv/Fm Performance

The maximum yield of the PSII photochemical reactions (Fv/Fm) reflects the maximum quantum efficiency of PSII photochemistry, and it has been widely used for early stress detection in plants [27]. Using a chlorophyll fluorometer, we first examined whether this mutation could affect the Fv/Fm. The results showed that there were no reliable changes in Fv/Fm under normal conditions. While Fv/Fm was characterized by a slight downward trend compared to the WT under drought stress conditions, the difference did not reach statistical significance (Figure 3A). Similarly, there were no significant changes in Fv/Fm after treatment with CaCl2, neither in the dorn1 mutant nor the WT (Figure 3A).

2.4. Exogenous CaCl2 Promotes the Photochemical Reaction Efficiency Performance in dorn1 Plants under Drought Stress

To further investigate the function of DORN1 in the actual photochemical efficiency of plants, we examined the major photosynthetic index under normal and drought conditions. Y(II) denotes the effective quantum yield of PSII, which is an indicator of the actual photochemical activity of PSII [28]. The results showed that no difference in the Y(II) of the dorn1 mutant compared with the WT under normal growth conditions. It is worth noting that Y(II) was significantly reduced in the dorn1 mutant under drought conditions, although no visible changes were observed in the WT under drought conditions. However, after treatment with CaCl2, Y(II) of the dorn1 mutant increased significantly and returned to WT levels (Figure 3B). The photochemical quenching coefficient (qP) indicates a fraction of “open” PSII reaction centers based on a puddle model [29,30]. In the dorn1 mutant, qP was significantly reduced when compared with WT under drought conditions, but this decrease was effectively delayed or even improved to the WT levels by the exogenous CaCl2 treatment (Figure 3C).
The actual photochemical efficiency of PSII is considered a reasonable index of the quantum yield of photosynthetic electron transport. To perform these analyses Next, we examined the rate of photosynthetic electron transport directly through PSII (ETR). The results showed that ETR had the same changing tendency as the Y(II) and qP. Drought treatments resulted in a significant reduction of ETR levels in the dorn1 mutant, and CaCl2 treatment significantly recovered ETR levels in the dorn1 mutant under drought stress conditions. whereas there was no obvious effect on the ETR levels of WT plants under similar treatment (Figure 3D).

2.5. Photooxidative Damage Increased in the dorn1 Plant under Drought Stress

The quantum yield of non-light-induced non-photochemical fluorescence quenching Y(NO) (also denoted as Φf.D) reflects the yield for other energy losses [29]. It is generally accepted that high Y(NO) indicates both photochemical energy conversion and protective regulatory mechanisms are inefficient. Plants may have serious problems coping with the incident radiation; either they had been damaged already or they would be photodamaged upon further irradiation [31]. Results showed that there was no visible change in Y(NO) between the dorn1 mutant and WT plants under normal growth conditions (Figure 4A). As a sensitive indicator of the plant stress response, Y(NO) increased substantially due to drought stress in the dorn1 mutant relative to the WT. Y(NO) was characterized by a slight downward trend in the dorn1 mutant by the exogenous CaCl2 treatment, rather than an increasing trend in the WT plants (Figure 4A).
Y(NPQ) represents the quantum yield of light-induced non-photochemical fluorescence quenching, without such dissipation there would be the formation of singlet oxygen and reactive radicals, which cause irreversible damage. On the one hand, a high Y(NPQ) value indicates that the photon flux density is excessive. On the other hand, it shows that the sample has retained the physiological means to protect itself by regulation. No change was observed in Y(NPQ) between the dorn1 mutant and WT plants under normal growth conditions (Figure 4B). The dorn1 mutant showed a clear decrease in Y(NPQ) relative to the WT under drought conditions, while it showed an increasing trend after treatment by the exogenous CaCl2 (Figure 4B). These results suggest that exogenous CaCl2 could alleviate photooxidative damage induced by drought stress in the dorn1 plant.

2.6. The dorn1 Plants Have a Greater Transpirational Water Loss

The dorn1 plants showed an accelerated decline in photosynthesis under drought stress conditions, indicating that drought tolerance performance in the mutant seedlings was impaired. We further confirmed this by phenotypic analysis of plants with direct drought treatment (Figure 2). It is known that transpirational water loss has a decisive effect on plant water metabolism and drought resistance in the plant. To investigate whether the reduced drought tolerance in this study may be attributed to transpirational water loss, we examined the rate of transpirational water loss rate both in WT and the dorn1 mutant. The two mutants’ transpiration water loss rates of the showed an increasing trend compared with the WT (Figure 5).

2.7. Exogenous CaCl2 Could Relieve Excessive Stomatal Opening in the dorn1 Mutants

Since more than 90% of water loss occurs through transpiration from the stomata in terrestrial plants [32,33]. We further analyzed the stomatal phenotypes in WT and the dorn1 mutant. As we expected, the stomatal aperture of the mutant was significantly increased compared with the WT (Figure 6A). Besides, stomatal opening showed a decreasing trend after treatment by the exogenous CaCl2 (Figure 6B). All these results suggested that excessive water loss caused by increased stomatal aperture is an important reason for the decreased drought tolerance of the dorn1 plants. Furthermore, according to the experimental results and previous reports, we also proposed a working model for the roles of DORN1 in plant drought response (Figure 6C).

3. Discussion

The eATP has attracted more and more attention since it was found to be a signaling molecule. In 2014, Choi et al. identified the first ATP receptor-DORN1 in the model plant Arabidopsis thaliana. eATP is recognized by DORN1 on the cell surface and dependents on downstream signaling pathways activation. Numerous studies have shown that eATP plays multiple roles in plant development and stress response [6], such as seed germination, photosynthesis, plant immune response, and salt tolerance. It was reported that these signaling pathways are closely related to the induction of intracellular second messengers, such as [Ca2+]cyt [5]. Drought is one of the most important and prevalent stress factors for plants. In the present study, we found that the photochemical efficiency of PSII parameters was significantly lower in dorn1 mutants than in WT under drought conditions. These results suggested that the drought tolerance of the mutant plants was decreased. In addition, exogenous CaCl2 alleviates drought stress on the photosynthetic apparatus of the plants. We also observed that the dorn1 mutants showed an increased water loss rate, which was accompanied by the opening status of leaf stomata, the main channel for water loss through transpiration. Moreover, the application of CaCl2 could effectively alleviate the excessive opening of stomata (Figure 6). Based on our results, it is attractive to hypothesize that DORN1 plays a vital role in drought stress tolerance in Arabidopsis thaliana, and this may be associated with [Ca2+]cyt.-induced stomatal closure.
Although no obvious phenotype was observed in the dorn1 mutants compared with WT when grown under optimal growth conditions. The latest study has shown that Arabidopsis lectin receptor kinase P2K2 is a second plant receptor for extracellular ATP and contributes to innate immunity [34]. These findings suggest that there may be functional redundancy between the two eATP receptors, which could explain the normal phenotype of the dorn1 mutants under optimal growth conditions. A recent report elucidated that DORN1 may be an important signal that helps mediate water homeostasis [35], especially under conditions of drought. Here we found that drought tolerance was significantly reduced in the dorn1 mutants, our results confirmed the conclusion from previous research that DORN1 plays a role in water homeostasis [35]. Plants regularly face adverse growth conditions, such as drought and plant disease. Chlorophyll fluorescence is a powerful and widely used indicator of the physiological status of plants under abiotic or biotic stress [25]. We found Y(II), qP and ETR showed a significant reduction in the dorn1 mutant under drought stress conditions, while exogenous CaCl2 could alleviate drought stress on the photosynthetic apparatus of these parameters. These results suggested that the loss function of DORN1 led to decreased photosynthetic capacity in plants, which might be related to [Ca2+]cyt. Y(NO) is considered an indicator for PSII photodamage. Under normal conditions, the rate of repair of PS II is balanced with the rate of damage. Drought stress reduced the rate of repair and disturbed the dynamic balance, which led to the decreased activity of PS II. Therefore, it is also worth investigating whether overexpression of DORN1 can promote drought tolerance in plants. Furthermore, CaCl2 could reduce the increased Y(NO) caused by drought stress in the dorn1 mutant. This finding suggested the dorn1 mutant exhibited more severe photo or oxidative damage under drought conditions, and this damage is also closely related to [Ca2+]cyt.
Studies have shown [Ca2+]cyt as a specific second messenger through the plasma membrane DORN1 receptor in plants [36,37]. eATP could cause the production of reactive oxygen species by NADPH oxidase AtRBOHC/AtRBOHD [6]. This may further activate calcium channels in the plasma membrane, which contributes to the elevation of cytosolic-free Ca2+ [7]. This is also associated with the phenomenon of Ca2+ waves, in which a [Ca2+]cyt signal could propagate along with tissues [14,38]. The Ca2+ level in the dorn1 mutant was significantly lower than that in the wild type after ATP treatment [14]. Moreover, [Ca2+]cyt is critical for stomatal movement, application of exogenous Ca2+ to plant tissue induced stomatal closure [39,40], external calcium elicited cytosolic calcium oscillations, and induced stomatal closure. Consistent with previous reports, our results showed that exogenous Ca2+ alleviated the stomatal aperture in the mutant deficient in DORN1. Calcium-permeable channels in the plasma membrane (PM) of guard cells play an important role in Ca2+-regulated stomatal closure [40]. Therefore, the relationship between DORN1 and calcium channel proteins deserves further discussion. Studies have shown that exogenous calcium effectively improves plant photosynthesis under heat or cold stress conditions [41,42]. Similarly, exogenous CaCl2 treatment significantly enhanced Y(II), qP, and ETR levels in the dorn1 mutant under drought stress conditions. Besides, the increase in Y(NO) in the dorn1 mutant was also alleviated by exogenous CaCl2 treatment. These findings showed that Ca2+ is essential in restoring the rate of repair and disturbs the dynamic balance of PS II in the dorn1 mutant drought stress condition.
We show that DORN1 regulates drought avoidance via modulating [Ca2+]cyt.-induced stomatal closure, which may provide a link between the DORN1-associated eATP signaling pathway and plant drought stress tolerance.

4. Materials and Methods

4.1. Plant Materials and Growth Condition

All Arabidopsis plants used in the current study were in the Columbia-0 background. The seeds were surface sterilized with 75% ethanol containing 0.05% Tween-20. The seeds were placed on plates that contained 1/2 MS medium (Duchefa, M0222) supplemented with 0.6% agar (Sangon Biotech, A505255) and 1% sucrose (Sangon Biotech, A502792), and then stratified at 4 °C for 2 days in darkness. The plates were then placed for seed germination and plant growth in a growth chamber set at 22 °C under 110 ± 5 μmol m−2 s−1 light of long-day conditions with a 16 h light/8 h dark cycle, then plants transferred to nutrient soil after one week of growth. For the CaCl2 treatment, seedlings were grown on nutrient soil for the indicated number of days, and then 1 mM CaCl2 was sprayed on the leaves once per day (1 mL per plant).

4.2. Construction of CRISPR/Cas9 Plant Expression Vectors

Nucleotide sequence encoding for DORN1 (AT5G60300) was retrieved from the Arabidopsis Information Resource (https://www.arabidopsis.org/, accessed on 20 December 2017). Synthetic guide RNA (AT5G60300-sgRNA:5′-GATATTGACCACAATCATGT-3′) targeting the first exon was designed by the E-CRISP tool (http://www.e-crisp.org, (accessed on 20 December 2017). The oligomer of designed sgRNA was synthesized and cloned into the pKSE401 binary vector carrying the Cas9 gene (Addgene plasmid # 62202).

4.3. Identification of the Homozygous Mutant Plants

Genomic DNA was extracted from the positive T1 plants’ leaves, as the template for PCR reaction. The DNA was amplified with the forward primer DORN1-F 5′-CAGTTCATCTGGACCACTCT-3′; Reverse primer DORN1-R 5′-CACTGCTATTTCTCTGCCTT-3′. The PCR-amplified fragment of each DNA sample was sequenced and compared to the gene sequence of DORN1 in WT by SnapGene software (www.snapgene.com, (accessed on 17 May 2018).

4.4. Measurement of Chlorophyll Fluorescence

Chlorophyll fluorescence parameters were measured using Image-PAM with a MAXI measuring head (Image–PAM, WALZ, Effeltrich, Germany), as described previously by Demmig-Adams [43]. Fv/Fm, the PSII maximal photochemical efficiency was defined as (Fm − F0)/Fm, where Fm is the maximum fluorescence emission from the dark-adapted state measured with a pulse of saturating light and F0 is the minimal fluorescence from the dark-adapted state. Y(II), the effective quantum yield of PSII photochemistry was defined as (Fm’ − Fs)/Fm’, where Fm’ is the maximum fluorescence emission from the light-adapted state measured with a pulse of saturating light and Fs is the steady-state level of fluorescence emission at 110 μmol m−2 s−1. qP, was defined as (Fm’ − Fs)/(Fm’ − F0’) according to Lazár [29]. As Y(II) represents the number of electrons transferred per photons absorbed by PSII, the ETR (the rate of photosynthetic electron transport through PSII can be calculated as ETR = Y(II) × PAR × 0.5 × 0.84 as per Genty [44]. Y(NO), the quantum yield of non-regulated energy dissipation of PSII, and Y(NPQ), the quantum yield of regulated energy dissipation of PSII were also defined by Kramer [31].

4.5. Measurement of Transpirational Water Loss

Water loss was measured as described previously with minor modifications [45]. Shoots of four-week-old relative to the WT and dorn1-c3/c6 were detached from plants cultivated in the growth chamber and quickly weighed to obtain fresh weights (W0). The shoots were then placed in filter paper under the light intensity of 110 ± 5 μmol m−2 s−1 at 25 °C and 50% relative humidity. The weight was recorded every 30 min (W1, W2, W3, W4), and the loss of fresh weight ([W0 − W1]/W0, [W 0− W2,]/W0, [W0 − Wn…]/W0,) was used to indicate the transpirational water loss rate.

4.6. Measurement of Stomatal Closure in Response to CaCl2 and Drought Treatment

The leaves from the same position within the rosette were preserved in the wash solution (ethanol/acetic acid, 19:1) at room temperature for 3–5 h, washed with distilled water, and placed in the clear solution (glycerol/chloral hydrate/water, 1:8:1, vol/wt/vol) overnight or for a few days [46]. Four images at 400× magnification were captured per leaf using a Zeiss LSM 880 NLO system (Carl Zeiss Microscopy GmbH, Jena, Germany) with a 40× water objective and processed using Adobe Photoshop. Stomatal aperture width and length were measured from the images using the Image J software (ImageJ nih.gov).

4.7. Statistical Analysis

The results were expressed as the mean ± standard deviation. The data were statistically evaluated by one-way ANOVA between the different groups and Tukey’s multiple comparisons test; The difference was considered to be statistically significant when p < 0.05.

Author Contributions

Q.W. and Q.J. conceived the work; Q.W. designed the experimental and analytical approaches; Q.W. and H.B. performed the experiments and data analysis; Q.W., H.B., and Q.J. discussed the results; A.Z., Q.W. and Q.J. wrote the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This research was supported by the National Natural Science Foundation of China (32200429), Entrepreneurship and Innovation Program Fund of Jiangsu Province (JSSCBS20211026), Yangzhou “Green Yang Jin Feng Project” excellent doctor fund (YZLYJFJH2021YXBS161).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

This work was supported by Scientific Research Start-up Fund at Yangzhou University. The Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University and National Key Laboratory of Crop Genetics and Germplasm Enhancement and Nanjing Agricultural University, China provided preliminary experiments.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Roelfsema, M.R.G.; Hedrich, R. Making sense out of Ca2+ signals: Their role in regulating stomatal movements. Plant Cell Environ. 2010, 33, 305–321. [Google Scholar] [CrossRef] [PubMed]
  2. Wang, W.H.; Chen, J.; Liu, T.W.; Chen, J.; Han, A.D.; Simon, M.; Dong, X.J.; He, J.X.; Zheng, H.L. Regulation of the calcium-sensing receptor in both stomatal movement and photosynthetic electron transport is crucial for water use efficiency and drought tolerance in Arabidopsis. J. Exp. Bot. 2014, 65, 223–234. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  3. Yoo, C.Y.; Mano, N.; Finkler, A.; Weng, H.; Day, I.S.; Reddy, A.S.N.; Poovaiah, B.W.; Fromm, H.; Hasegawa, P.M.; Mickelbart, M.V. A Ca2+/CaM-regulated transcriptional switch modulates stomatal development in response to water deficit. Sci. Rep. 2019, 9, 12282. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Roux, S.J.; Steinebrunner, I. Extracellular ATP: An unexpected role as a signaler in plants. Trends Plant Sci. 2007, 12, 522–527. [Google Scholar] [CrossRef] [PubMed]
  5. Matthus, E.; Sun, J.; Wang, L.M.; Bhat, M.G.; Mohammad-Sidik, A.B.; Wilkins, K.A.; Leblanc-Fournier, N.; Legue, V.; Moulia, B.; Stacey, G.; et al. DORN1/P2K1 and purino-calcium signalling in plants: Making waves with extracellular ATP. Ann. Bot. 2019, 124, 1227–1242. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  6. Cao, Y.R.; Tanaka, K.; Nguyen, C.T.; Stacey, G. Extracellular ATP is a central signaling molecule in plant stress responses. Curr. Opin. Plant Biol. 2014, 20, 82–87. [Google Scholar] [CrossRef] [PubMed]
  7. Wang, L.; Wilkins, K.A.; Davies, J.M. Arabidopsis DORN1 extracellular ATP receptor; activation of plasma membrane K+ -and Ca2+-permeable conductances. New Phytol. 2018, 218, 1301–1304. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  8. Mohammad-Sidik, A.; Sun, J.; Shin, R.; Song, Z.Z.; Ning, Y.Z.; Matthus, E.; Wilkins, K.A.; Davies, J.M. Annexin 1 Is a Component of eATP-Induced Cytosolic Calcium Elevation in Arabidopsis thaliana Roots. Int. J. Mol. Sci. 2021, 22, 494. [Google Scholar] [CrossRef]
  9. Moeder, W.; Phan, V.; Yoshioka, K. Ca2+ to the rescue—Ca2+ channels and signaling in plant immunity. Plant Sci. 2019, 279, 19–26. [Google Scholar] [CrossRef]
  10. Dong, Q.; Wallrad, L.; Almutairi, B.O.; Kudla, J. Ca2+ signaling in plant responses to abiotic stresses. J. Integr. Plant Biol. 2022, 64, 287–300. [Google Scholar] [CrossRef]
  11. Du, L.Q.; Poovaiah, B.W. Ca2+/calmodulin is critical for brassinosteroid biosynthesis and plant growth. Nature 2005, 437, 741–745. [Google Scholar] [CrossRef] [PubMed]
  12. Yuan, P.; Du, L.; Poovaiah, B.W. Ca2+/Calmodulin-Dependent AtSR1/CAMTA3 Plays Critical Roles in Balancing Plant Growth and Immunity. Int. J. Mol. Sci. 2018, 19, 1764. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Schiøtt, M.; Romanowsky, S.M.; Bækgaard, L.; Jakobsen, M.K.; Palmgren, M.G.; Harper, J.F. A plant plasma membrane Ca2+ pump is required for normal pollen tube growth and fertilization. Proc. Natl. Acad. Sci. USA 2004, 101, 9502–9507. [Google Scholar] [CrossRef] [Green Version]
  14. Choi, J.; Tanaka, K.; Cao, Y.; Qi, Y.; Qiu, J.; Liang, Y.; Lee, S.Y.; Stacey, G. Identification of a plant receptor for extracellular ATP. Science 2014, 343, 290–294. [Google Scholar] [CrossRef] [PubMed]
  15. Tripathi, D.; Zhang, T.; Koo, A.J.; Stacey, G.; Tanaka, K. Extracellular ATP Acts on Jasmonate Signaling to Reinforce Plant Defense. Plant Physiol. 2018, 176, 511–523. [Google Scholar] [CrossRef] [Green Version]
  16. Kumar, S.; Tripathi, D.; Okubara, P.A.; Tanaka, K. Purinoceptor P2K1/DORN1 Enhances Plant Resistance Against a Soilborne Fungal Pathogen, Rhizoctonia solani. Front. Plant Sci. 2020, 11, 572920. [Google Scholar] [CrossRef]
  17. Tanaka, K.; Choi, J.M.; Cao, Y.R.; Stacey, G. Extracellular ATP acts as a damage-associated molecular pattern (DAMP) signal in plants. Front. Plant Sci. 2014, 5, 446. [Google Scholar] [CrossRef] [Green Version]
  18. Nizam, S.; Qiang, X.Y.; Wawra, S.; Nostadt, R.; Getzke, F.; Schwanke, F.; Dreyer, I.; Langen, G.; Zuccaro, A. Serendipita indica E5’NT modulates extracellular nucleotide levels in the plant apoplast and affects fungal colonization. EMBO Rep. 2019, 20, e47430. [Google Scholar] [CrossRef]
  19. Hou, Q.Z.; Sun, K.; Zhang, H.; Su, X.; Fan, B.Q.; Feng, H.Q. The responses of photosystem II and intracellular ATP production of Arabidopsis leaves to salt stress are affected by extracellular ATP. J. Plant Res. 2018, 131, 331–339. [Google Scholar] [CrossRef]
  20. Shan, Y.X.; Li, F.J.; Lian, Q.Q.; Xie, L.H.; Zhu, H.; Li, T.T.; Zhang, J.; Duan, X.W.; Jiang, Y.M. Role of apyrase-mediated eATP signal in chilling injury of postharvest banana fruit during storage. Postharvest Biol. Technol. 2022, 187, 111874. [Google Scholar] [CrossRef]
  21. Jiao, Q.S.; Feng, H.Q.; Tian, W.Y.; Bai, J.Y.; Sun, K.; Jia, L.Y.; Zhang, J.P. Extracellular ATP functions in alleviating the decrease of PSII photochemistry caused by the infection of Xanthomonas campestris pv. phaseoli. Plant Pathol. 2016, 65, 819–825. [Google Scholar] [CrossRef]
  22. Chivasa, S.; Murphy, A.M.; Hamilton, J.M.; Lindsey, K.; Carr, J.P.; Slabas, A.R. Extracellular ATP is a regulator of pathogen defence in plants. Plant J. 2009, 60, 436–448. [Google Scholar] [CrossRef] [PubMed]
  23. Kalaji, H.M.; Baba, W.; Gediga, K.; Goltsev, V.; Samborska, I.A.; Cetner, M.D.; Dimitrova, S.; Piszcz, U.; Bielecki, K.; Karmowska, K.; et al. Chlorophyll fluorescence as a tool for nutrient status identification in rapeseed plants. Photosynth. Res. 2018, 136, 329–343. [Google Scholar] [CrossRef] [Green Version]
  24. Lotfi, R.; Abbasi, A.; Kalaji, H.M.; Eskandari, I.; Sedghieh, V.; Khorsandi, H.; Sadeghian, N.; Yadav, S.; Rastogi, A. The role of potassium on drought resistance of winter wheat cultivars under cold dryland conditions: Probed by chlorophyll a fluorescence. Plant Physiol. Biochem. 2022, 182, 45–54. [Google Scholar] [CrossRef]
  25. Murchie, E.H.; Lawson, T. Chlorophyll fluorescence analysis: A guide to good practice and understanding some new applications. J. Exp. Bot. 2013, 64, 3983–3998. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  26. Venios, X.; Korkas, E.; Nisiotou, A.; Banilas, G. Grapevine Responses to Heat Stress and Global Warming. Plants 2020, 9, 1754. [Google Scholar] [CrossRef]
  27. Schansker, G.; Tóth, S.Z.; Holzwarth, A.R.; Garab, G.J.P.R. Chlorophyll a fluorescence: Beyond the limits of the QA model. Photosynth. Res. 2014, 120, 43–58. [Google Scholar] [CrossRef]
  28. Jiao, Q.S.; Niu, G.T.; Wang, F.; Dong, J.Y.; Hong, Z. N-glycosylation regulates photosynthetic efficiency of Arabidopsis thaliana. Photosynthetica 2019, 58, 72–79. [Google Scholar] [CrossRef] [Green Version]
  29. Lazar, D. Parameters of photosynthetic energy partitioning. J. Plant Physiol. 2015, 175, 131–147. [Google Scholar] [CrossRef]
  30. Stirbet, A. Excitonic connectivity between photosystem II units: What is it, and how to measure it? Photosynth. Res. 2013, 116, 189–214. [Google Scholar] [CrossRef]
  31. Kramer, D.M.; Johnson, G.; Kiirats, O.; Edwards, G.E. New Fluorescence Parameters for the Determination of Q A Redox State and Excitation Energy Fluxes. Photosynth. Res. 2004, 79, 209–218. [Google Scholar] [CrossRef] [PubMed]
  32. Tanaka, Y.; Nose, T.; Jikumaru, Y.; Kamiya, Y. ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves. Plant J. 2013, 74, 448–457. [Google Scholar] [CrossRef] [PubMed]
  33. Spiegelhalder, R.P.; Raissig, M.T. Morphology made for movement: Formation of diverse stomatal guard cells. Curr. Opin. Plant Biol. 2021, 63, 102090. [Google Scholar] [CrossRef] [PubMed]
  34. Pham, A.Q.; Cho, S.H.; Nguyen, C.T.; Stacey, G. Arabidopsis Lectin Receptor Kinase P2K2 Is a Second Plant Receptor for Extracellular ATP and Contributes to Innate Immunity. Plant Physiol. 2020, 183, 1364–1375. [Google Scholar] [CrossRef]
  35. Chen, D.; Cao, Y.; Li, H.; Kim, D.; Ahsan, N.; Thelen, J.; Stacey, G. Extracellular ATP elicits DORN1-mediated RBOHD phosphorylation to regulate stomatal aperture. Nat. Commun. 2017, 8, 2265. [Google Scholar] [CrossRef] [Green Version]
  36. Matthus, E.; Wilkins, K.A.; Mohammad-Sidik, A.; Ning, Y.; Davies, J.M. Spatial origin of the extracellular ATP-induced cytosolic calcium signature in Arabidopsis thaliana roots: Wave formation and variation with phosphate nutrition. Plant Biol. 2022, 24, 863–873. [Google Scholar] [CrossRef]
  37. Song, C.J.; Steinebrunner, I.; Wang, X.; Stout, S.C.; Roux, S.J. Extracellular ATP induces the accumulation of superoxide via NADPH oxidases in Arabidopsis. Plant Physiol. 2006, 140, 1222–1232. [Google Scholar] [CrossRef] [Green Version]
  38. Evans, M.J.; Choi, W.G.; Gilroy, S.; Morris, R.J. A ROS-Assisted Calcium Wave Dependent on the AtRBOHD NADPH Oxidase and TPC1 Cation Channel Propagates the Systemic Response to Salt Stress. Plant Physiol. 2016, 171, 1771–1784. [Google Scholar] [CrossRef] [Green Version]
  39. Rhaman, M.S.; Nakamura, T.; Nakamura, Y.; Munemasa, S.; Murata, Y. The Myrosinases TGG1 and TGG2 Function Redundantly in Reactive Carbonyl Species Signaling in Arabidopsis Guard Cells. Plant Cell Physiol. 2020, 61, 967–977. [Google Scholar] [CrossRef]
  40. Tan, Y.-Q.; Yang, Y.; Shen, X.; Zhu, M.; Shen, J.; Zhang, W.; Hu, H.; Wang, Y.-F. Multiple cyclic nucleotide-gated channels function as ABA-activated Ca2+ channels required for ABA-induced stomatal closure in Arabidopsis. Plant Cell 2022. [Google Scholar] [CrossRef]
  41. Zhang, G.; Liu, Y.; Ni, Y.; Meng, Z.; Lu, T.; Li, T. Exogenous calcium alleviates low night temperature stress on the photosynthetic apparatus of tomato leaves. PLoS ONE 2014, 9, e97322. [Google Scholar] [CrossRef] [PubMed]
  42. Tan, W.; Meng, Q.W.; Brestic, M.; Olsovska, K.; Yang, X.H. Photosynthesis is improved by exogenous calcium in heat-stressed tobacco plants. J. Plant Physiol. 2011, 168, 2063–2071. [Google Scholar] [CrossRef] [PubMed]
  43. Demmig-Adams, B.; Adams Iii, W.W.; Barker, D.H.; Logan, B.A.; Bowling, D.R.; Verhoeven, A.S. Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation. Physiol. Plant. 1996, 98, 253–264. [Google Scholar] [CrossRef]
  44. Genty, B.; Briantais, J.-M.; Baker, N.R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim. Biophys. Acta (BBA)-Gen. Subj. 1989, 990, 87–92. [Google Scholar] [CrossRef]
  45. Jiao, Q.; Chen, T.; Niu, G.; Zhang, H.; Zhou, C.; Hong, Z. N-glycosylation is involved in stomatal development by modulating the release of active abscisic acid and auxin in Arabidopsis. J. Exp. Bot. 2020, 71, 5865–5879. [Google Scholar] [CrossRef]
  46. Zhang, J.Y.; He, S.B.; Li, L.; Yang, H.Q. Auxin inhibits stomatal development through MONOPTEROS repression of a mobile peptide gene STOMAGEN in mesophyll. Proc. Natl. Acad. Sci. USA 2014, 111, E3015–E3023. [Google Scholar] [CrossRef]
Ijms 23 14213 g001 550
Figure 1. The dorn1 mutants showed no obvious phenotype under normal growth conditions. (A) Gene structure of DORN1 and the Cas9/guide RNA target site. (B) Mutation site information of the dorn1-c3 and dorn1-c6, dorn1-c3 characterized a 10 bp deletion and dorn1-c6 with 7 bp insertion/8 bp deletion. (C) The dorn1 mutants grew well under normal culture conditions. (D) Statistical analysis of fresh plant weight. Each data point represents the average fresh weight of ~15 seedlings of three duplicate experiments, and error bars represent the standard deviation (SD).
Figure 1. The dorn1 mutants showed no obvious phenotype under normal growth conditions. (A) Gene structure of DORN1 and the Cas9/guide RNA target site. (B) Mutation site information of the dorn1-c3 and dorn1-c6, dorn1-c3 characterized a 10 bp deletion and dorn1-c6 with 7 bp insertion/8 bp deletion. (C) The dorn1 mutants grew well under normal culture conditions. (D) Statistical analysis of fresh plant weight. Each data point represents the average fresh weight of ~15 seedlings of three duplicate experiments, and error bars represent the standard deviation (SD).
Ijms 23 14213 g001
Ijms 23 14213 g002 550
Figure 2. Phenotypic analysis of drought tolerance in the dorn1 mutant. (A) Phenotype of 7-day seedlings treated with different periods of drought and rehydration. (B) The survival rate of different plants after rehydration, different letters indicate significant differences (p < 0.05) using ANOVA analysis. Each data point represents the average survival rate of ~15 seedlings of three duplicate experiments, and error bars represent the standard deviation (SD).
Figure 2. Phenotypic analysis of drought tolerance in the dorn1 mutant. (A) Phenotype of 7-day seedlings treated with different periods of drought and rehydration. (B) The survival rate of different plants after rehydration, different letters indicate significant differences (p < 0.05) using ANOVA analysis. Each data point represents the average survival rate of ~15 seedlings of three duplicate experiments, and error bars represent the standard deviation (SD).
Ijms 23 14213 g002
Ijms 23 14213 g003 550
Figure 3. Effects of CaCl2 on the photochemical reaction performance of the dorn1 plant under drought stress. Effects of CaCl2 on the performance of Fv/Fm (A), Y(II) (B), qP(C) and ETR (D) in the dorn1 mutant and WT plants. The treatment group was pre-treated by spraying 1 mM CaCl2 once a day for three days. Different letters indicate significant differences (p < 0.05) using ANOVA analysis. Each data point represents the average data of ~15 seedlings of three duplicate experiments, and error bars represent the standard deviation (SD).
Figure 3. Effects of CaCl2 on the photochemical reaction performance of the dorn1 plant under drought stress. Effects of CaCl2 on the performance of Fv/Fm (A), Y(II) (B), qP(C) and ETR (D) in the dorn1 mutant and WT plants. The treatment group was pre-treated by spraying 1 mM CaCl2 once a day for three days. Different letters indicate significant differences (p < 0.05) using ANOVA analysis. Each data point represents the average data of ~15 seedlings of three duplicate experiments, and error bars represent the standard deviation (SD).
Ijms 23 14213 g003
Ijms 23 14213 g004 550
Figure 4. Effects of CaCl2 on photooxidative damage of the dorn1 plant under drought stress. Effects of CaCl2 on Y(NO) (A) and Y(NPQ) (B) performance in the dorn1 mutant and WT plants. The treatment group was pre-treated by spraying 1 mM CaCl2 once a day for three days. Different letters indicate significant differences (p < 0.05) using ANOVA analysis. Each data point represents the average data of ~15 seedlings of three duplicate experiments, and error bars represent the standard deviation (SD).
Figure 4. Effects of CaCl2 on photooxidative damage of the dorn1 plant under drought stress. Effects of CaCl2 on Y(NO) (A) and Y(NPQ) (B) performance in the dorn1 mutant and WT plants. The treatment group was pre-treated by spraying 1 mM CaCl2 once a day for three days. Different letters indicate significant differences (p < 0.05) using ANOVA analysis. Each data point represents the average data of ~15 seedlings of three duplicate experiments, and error bars represent the standard deviation (SD).
Ijms 23 14213 g004
Ijms 23 14213 g005 550
Figure 5. The transpirational water loss of the dorn1 and WT plants. Mutant and WT plants are grown on soil for 21 days, then the water loss rate is measured and analyzed. Each data point represents the average water loss of ~15 seedlings of three duplicate experiments, and error bars represent the standard deviation (SD). “*“ indicate significant differences (p < 0.05) using Tukey’s analysis.
Figure 5. The transpirational water loss of the dorn1 and WT plants. Mutant and WT plants are grown on soil for 21 days, then the water loss rate is measured and analyzed. Each data point represents the average water loss of ~15 seedlings of three duplicate experiments, and error bars represent the standard deviation (SD). “*“ indicate significant differences (p < 0.05) using Tukey’s analysis.
Ijms 23 14213 g005
Ijms 23 14213 g006 550
Figure 6. Effects of CaCl2 on stomatal aperture. (A) Stomatal phenotype analysis of the plants with or without 1 mM CaCl2 treatment, for the treatment group, plants were pre-treated by spraying 1 mM CaCl2 one hour before decoloring. (B) Statistical analysis of the stomatal aperture (width/length). Different letters indicate significant differences (p < 0.05) using ANOVA analysis. Each data point represents the average stomatal aperture of ~15 seedlings of three duplicate experiments, and error bars represent standard deviation (SD). (C) A possible working model for DORN1 in drought tolerance in plants.
Figure 6. Effects of CaCl2 on stomatal aperture. (A) Stomatal phenotype analysis of the plants with or without 1 mM CaCl2 treatment, for the treatment group, plants were pre-treated by spraying 1 mM CaCl2 one hour before decoloring. (B) Statistical analysis of the stomatal aperture (width/length). Different letters indicate significant differences (p < 0.05) using ANOVA analysis. Each data point represents the average stomatal aperture of ~15 seedlings of three duplicate experiments, and error bars represent standard deviation (SD). (C) A possible working model for DORN1 in drought tolerance in plants.
Ijms 23 14213 g006
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Wang, Q.; Bai, H.; Zada, A.; Jiao, Q. DORN1 Is Involved in Drought Stress Tolerance through a Ca2+-Dependent Pathway. Int. J. Mol. Sci. 2022, 23, 14213. https://doi.org/10.3390/ijms232214213

AMA Style

Wang Q, Bai H, Zada A, Jiao Q. DORN1 Is Involved in Drought Stress Tolerance through a Ca2+-Dependent Pathway. International Journal of Molecular Sciences. 2022; 23(22):14213. https://doi.org/10.3390/ijms232214213

Chicago/Turabian Style

Wang, Qingwen, Hongbao Bai, Ahmad Zada, and Qingsong Jiao. 2022. "DORN1 Is Involved in Drought Stress Tolerance through a Ca2+-Dependent Pathway" International Journal of Molecular Sciences 23, no. 22: 14213. https://doi.org/10.3390/ijms232214213

APA Style

Wang, Q., Bai, H., Zada, A., & Jiao, Q. (2022). DORN1 Is Involved in Drought Stress Tolerance through a Ca2+-Dependent Pathway. International Journal of Molecular Sciences, 23(22), 14213. https://doi.org/10.3390/ijms232214213

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop