Diacylglycerol Acyltransferase 3(DGAT3) Is Responsible for the Biosynthesis of Unsaturated Fatty Acids in Vegetative Organs of Paeonia rockii
Abstract
:1. Introduction
2. Results
2.1. Molecular Characterization of DGAT3 from P. rockii
2.2. Expression Profile of PrDGAT3
2.3. Overexpression of PrDGAT3 Functionally Recovers oil Biosynthesis in TAG-deficient S. cerevisiae H1246
2.4. PrDGAT3 Manifests High Substrate Preference for LA and ALA Revealed by Yeast Lipid Toxicity Test
2.5. Transient Expression of PrDGAT3 Enhances oil and ALA Content in Nicotiana Benthamiana Leaves
2.6. Reduced Expression of PrDGAT3 in Paeonia rockii Represses the Oil Accumulation and Affects Fatty Acids Composition
2.7. Stable Expression of PrDGAT3 Increases Oil, Especially LA and ALA Accumulation, in Transgentic Arabidopsis Rosette Leaves
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Regents
4.2. The Cloning of PrDGAT3 and Sequence Analysis
4.3. Expression Analysis of PrDGAT3
4.4. Construction of PrDGAT3 Expression Vectors
4.5. Subcellular Location
4.6. Heterogeous Expression in the S. cerevisiae Mutant H1246 Strain
4.7. Yeast Fatty Acid Toxicity Test for acyl-CoA Substrate Specificity Assay
4.8. Lipid Extraction, Fatty Acid Analysis, and Visualization of Lipid Droplets
4.9. Transient Overexpression in Nicotiana Benthamiana Leaves
4.10. Virus-Induced Gene Silencing of PrDGAT3
4.11. Overexpression of PrDGAT3 in Arabidopsis Thaliana
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Han, L.; Zhai, Y.; Wang, Y.; Shi, X.; Xu, Y.; Gao, S.; Zhang, M.; Luo, J.; Zhang, Q. Diacylglycerol Acyltransferase 3(DGAT3) Is Responsible for the Biosynthesis of Unsaturated Fatty Acids in Vegetative Organs of Paeonia rockii. Int. J. Mol. Sci. 2022, 23, 14390. https://doi.org/10.3390/ijms232214390
Han L, Zhai Y, Wang Y, Shi X, Xu Y, Gao S, Zhang M, Luo J, Zhang Q. Diacylglycerol Acyltransferase 3(DGAT3) Is Responsible for the Biosynthesis of Unsaturated Fatty Acids in Vegetative Organs of Paeonia rockii. International Journal of Molecular Sciences. 2022; 23(22):14390. https://doi.org/10.3390/ijms232214390
Chicago/Turabian StyleHan, Longyan, Yuhui Zhai, Yumeng Wang, Xiangrui Shi, Yanfeng Xu, Shuguang Gao, Man Zhang, Jianrang Luo, and Qingyu Zhang. 2022. "Diacylglycerol Acyltransferase 3(DGAT3) Is Responsible for the Biosynthesis of Unsaturated Fatty Acids in Vegetative Organs of Paeonia rockii" International Journal of Molecular Sciences 23, no. 22: 14390. https://doi.org/10.3390/ijms232214390