Analysis of QTLs and Candidate Genes for Tassel Symptoms in Maize Infected with Sporisorium reilianum
Abstract
:1. Introduction
2. Results
2.1. Sporisorium reilianum Causes Damage to Maize Cell Structure
2.2. Identification of Tassel Symptoms in the Two F2 Populations
2.3. Identification of Polymorphisms between the Parents
2.4. Construction of Mixed Gene Pools and Selection of Primers
2.5. Preliminary Mapping of QTLs
2.6. Predicting Candidate Gene Related to Tassel Symptoms of Maize Infected with Sporisorium reilianum
3. Discussion
3.1. Differences in Tassel Symptoms Infected by Head Smut of Sipingtou Bloodlines of Maize
3.2. Advantage of Using the BSA Method to Analyze Tassel Symptoms in Maize Infected with Sporisorium reilianum
3.3. Genetic Study of Maize Tassels Infected with Sporisorium reilianum
3.4. Function of Two Candidate Genes Related to the Tassel Symptoms of Head Smut
4. Materials and Methods
4.1. Plant and Sporisorium reilianum Materials
4.2. Analysis of Cell Morphological Changes in Tassels Infected with Sporisorium reilianum
4.3. Artificial Inoculation and Phenotypic Investigation
4.4. Construction of a Mixed Gene Pool with Different Symptoms from the F2 Population
4.5. DNA Extraction and Screening of SSR Markers
4.6. Data Processing and Statistical Analysis
4.7. Candidate Genes Prediction and Bioinformatics Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Phenotype | Huangzao4×Jing7 F2 (Numbers) | Jing7×Chang7-2 F2 (Numbers) |
---|---|---|
A | 265 | - |
B | 234 | 146 |
C | - | 183 |
D | 890 | 263 |
Inbred Lines | Types of Tassel Symptoms | Blood and Pedigree | Average Incidence | Tassel Symptoms |
---|---|---|---|---|
Huangzao4 | A | Sipingtou group, widely used in China | 92.31% | Exhibits severe carbonization of tassels and shows black filament with many spores |
Jing7 | B | Sipingtou group, selfing of Jingzao7 (Huangzao4×luoxi3) | 89.58% | Tassels expand without spores |
Chang7-2 | C | Sipingtou group, single-cross hybrid Changdan7 (Huangzao4×Wei95) | 73.81% | Base of its tassel is afflicted, whereas the upper part develops normally |
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Zhou, Y.; Yao, M.; Wang, Q.; Zhang, X.; Di, H.; Zhang, L.; Dong, L.; Xu, Q.; Liu, X.; Zeng, X.; et al. Analysis of QTLs and Candidate Genes for Tassel Symptoms in Maize Infected with Sporisorium reilianum. Int. J. Mol. Sci. 2022, 23, 14416. https://doi.org/10.3390/ijms232214416
Zhou Y, Yao M, Wang Q, Zhang X, Di H, Zhang L, Dong L, Xu Q, Liu X, Zeng X, et al. Analysis of QTLs and Candidate Genes for Tassel Symptoms in Maize Infected with Sporisorium reilianum. International Journal of Molecular Sciences. 2022; 23(22):14416. https://doi.org/10.3390/ijms232214416
Chicago/Turabian StyleZhou, Yu, Minhao Yao, Qian Wang, Xiaoming Zhang, Hong Di, Lin Zhang, Ling Dong, Qingyu Xu, Xianjun Liu, Xing Zeng, and et al. 2022. "Analysis of QTLs and Candidate Genes for Tassel Symptoms in Maize Infected with Sporisorium reilianum" International Journal of Molecular Sciences 23, no. 22: 14416. https://doi.org/10.3390/ijms232214416