Piperine Attenuates Cigarette Smoke-Induced Oxidative Stress, Lung Inflammation, and Epithelial–Mesenchymal Transition by Modulating the SIRT1/Nrf2 Axis
Abstract
:1. Introduction
2. Results
2.1. CSE Exposure Results in the Loss of Cell Viability in Lung Epithelial Cells
2.2. Piperine Protects the CSE-Induced Cell Death in Lung Epithelial Cells
2.3. Piperine Curtails Oxidative Stress and Inflammation by Upregulating SIRT1, Nrf2 and Inhibiting NF-κB Translocation in Lung Epithelial Cells
2.4. Piperine Inhibits the Wnt Signaling in Lung Epithelial Cells
2.5. Piperine Diminishes Oxidative Stress, NF-κB, and Wnt Signaling Resulting in Inhibition of EMT in Lung Epithelial Cells
2.6. Piperine Restores the CS-Induced Altered Lung Mechanics in Mice
2.7. Piperine Inhibits The Release of Inflammatory Cytokines and Reduces Immune Cells Infiltration in Isolated BAL Fluid and Lung Tissues
2.8. Piperine Curtails CS-Induced Oxidative Stress and Inflammatory Signaling in Isolated Lung Tissues
2.9. Piperine Inhibits EMT Changes and Modulates SIRT1 Signaling in Isolated Lung Tissues
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Preparation of Aqueous Cigarette Smoke Extract (CSE)
4.3. Cell Viability Assay
4.4. Immunofluorescence
4.5. Cigarette Smoke Exposure in a Mouse Model
4.6. Assessment of Lung Function
4.7. Assessment of BAL Cytology
4.8. Measurement of Biochemical Parameters
4.9. RNA Isolation and Real-Time Polymerase Chain Reaction
4.10. Histopathological Analysis
4.11. Western Blotting
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Saha, P.; Durugkar, S.; Jain, S.; Shantanu, P.A.; Panda, S.R.; Jala, A.; Gokhale, S.; Sharma, P.; Naidu, V.G.M. Piperine Attenuates Cigarette Smoke-Induced Oxidative Stress, Lung Inflammation, and Epithelial–Mesenchymal Transition by Modulating the SIRT1/Nrf2 Axis. Int. J. Mol. Sci. 2022, 23, 14722. https://doi.org/10.3390/ijms232314722
Saha P, Durugkar S, Jain S, Shantanu PA, Panda SR, Jala A, Gokhale S, Sharma P, Naidu VGM. Piperine Attenuates Cigarette Smoke-Induced Oxidative Stress, Lung Inflammation, and Epithelial–Mesenchymal Transition by Modulating the SIRT1/Nrf2 Axis. International Journal of Molecular Sciences. 2022; 23(23):14722. https://doi.org/10.3390/ijms232314722
Chicago/Turabian StyleSaha, Pritam, Sneha Durugkar, Siddhi Jain, P. A. Shantanu, Samir R. Panda, Aishwarya Jala, Sharad Gokhale, Pawan Sharma, and V. G. M. Naidu. 2022. "Piperine Attenuates Cigarette Smoke-Induced Oxidative Stress, Lung Inflammation, and Epithelial–Mesenchymal Transition by Modulating the SIRT1/Nrf2 Axis" International Journal of Molecular Sciences 23, no. 23: 14722. https://doi.org/10.3390/ijms232314722