In Vitro Combination of Ascorbic and Ellagic Acids in Sperm Oxidative Damage Inhibition
Abstract
:1. Introduction
2. Results
2.1. Semen Analysis
2.2. Sperm Parameters after Exposure to Different Substances
2.3. DNA Fragmentation
2.4. Intracellular ROS Detection
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Sample Collection
4.3. Semen Analysis and Exposure Procedure
4.4. Sperm DNA Fragmentation (SDF)
4.5. DCF Assay
4.6. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sperm Parameters | Mean ± SD |
---|---|
Semen volume (mL) | 3.25 ± 1.31 |
pH | 7.70 ± 0.50 |
Sperm concentration (×106 sperm/mL) | 76.62 ± 25.36 |
Vitality (%) | 74.50 ± 6.20 |
Motility (%) | |
Progressive (P) | 32.35 ± 16.04 |
Non-progressive (NP) | 16.25 ± 7.97 |
Substances Concentration | Exposure Minutes | Motility (P + NP) (%) | Vitality (%) |
---|---|---|---|
AA 100 µM | 45 | 48.55 ± 7.40 | 78.20 ± 0.30 |
60 | 45.30 ± 6.90 | 72.50 ± 0.60 | |
90 | 38.50 ± 4.60 | 68.80 ± 2.00 | |
EA 100 µM | 45 | 47.00 ± 5.10 | 76.00 ± 1.25 |
60 | 43.80 ± 8.50 | 74.30 ± 1.70 | |
90 | 38.00 ± 9.80 | 69.00 ± 3.50 | |
BZ 0.4 µL/mL | 45 | 30.00 ± 5.70 * | 70.00 ± 1.50 |
60 | 25.35 ± 6.80 * | 66.50 ± 1.80 | |
90 | 17.85 ± 4.40 * | 53.20 ± 2.50 * | |
AA 100 µM + BZ 0.4 µL/mL | 45 | 47.00 ± 3.70 | 77.50 ± 3.20 |
60 | 42.60 ± 2.40 | 71.35 ± 3.50 | |
90 | 38.00 ± 5.20 | 66.80 ± 4.20 | |
EA 100 µM + BZ 0.4 µL/mL | 45 | 45.80± 4.50 | 75.00 ± 1.00 |
60 | 43.00 ± 6.70 | 72.80 ± 0.20 | |
90 | 37.60 ± 8.30 | 67.50 ± 2.40 | |
AA 100 µM + EA 100 µM | 45 | 48.00 ± 1.50 | 75.10 ± 0.50 |
60 | 44.50 ± 2.65 | 71.00 ± 2.00 | |
90 | 38.80 ± 4.80 | 69.50 ± 1.50 | |
AA 100 µM + EA 100 µM + BZ 0.4 µL/mL | 45 | 46.90 ± 5.20 | 75.30 ± 2.60 |
60 | 44.75 ± 1.50 | 72.60 ± 0.55 | |
90 | 40.35 ± 4.60 | 68.77 ± 3.45 |
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Mottola, F.; Iovine, C.; Carannante, M.; Santonastaso, M.; Rocco, L. In Vitro Combination of Ascorbic and Ellagic Acids in Sperm Oxidative Damage Inhibition. Int. J. Mol. Sci. 2022, 23, 14751. https://doi.org/10.3390/ijms232314751
Mottola F, Iovine C, Carannante M, Santonastaso M, Rocco L. In Vitro Combination of Ascorbic and Ellagic Acids in Sperm Oxidative Damage Inhibition. International Journal of Molecular Sciences. 2022; 23(23):14751. https://doi.org/10.3390/ijms232314751
Chicago/Turabian StyleMottola, Filomena, Concetta Iovine, Maria Carannante, Marianna Santonastaso, and Lucia Rocco. 2022. "In Vitro Combination of Ascorbic and Ellagic Acids in Sperm Oxidative Damage Inhibition" International Journal of Molecular Sciences 23, no. 23: 14751. https://doi.org/10.3390/ijms232314751