MicroRNA-200c Affects Milk Fat Synthesis by Targeting PANK3 in Ovine Mammary Epithelial Cells
Abstract
:1. Introduction
2. Results
2.1. MiR-200c Is Associated with Development and Lactation of Mammary Gland
2.2. miR-200c Affects the Proliferation of Ovine MECs
2.3. PANK3 Is a Direct Target Gene of miR-200c
2.4. miR-200c Affects the Milk Synthesis Process by Regulating the Expression of PANK3
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Collection of Ovine Tissue Samples
4.3. Isolation and Culture of Ovine MECs
4.4. Transfection of RNA Oligo
4.5. Cell Proliferation Assay
4.6. Target Predictions and Luciferase Activity Assay
4.7. RT-qPCR and Western Blot
4.8. Triglycerides Content Detection
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hao, Z.; Wang, J.; Luo, Y.; Hu, J.; Liu, X.; Li, S.; Li, M.; Shi, B.; Hu, L.; Liu, Y.; et al. MicroRNA-200c Affects Milk Fat Synthesis by Targeting PANK3 in Ovine Mammary Epithelial Cells. Int. J. Mol. Sci. 2022, 23, 15601. https://doi.org/10.3390/ijms232415601
Hao Z, Wang J, Luo Y, Hu J, Liu X, Li S, Li M, Shi B, Hu L, Liu Y, et al. MicroRNA-200c Affects Milk Fat Synthesis by Targeting PANK3 in Ovine Mammary Epithelial Cells. International Journal of Molecular Sciences. 2022; 23(24):15601. https://doi.org/10.3390/ijms232415601
Chicago/Turabian StyleHao, Zhiyun, Jiqing Wang, Yuzhu Luo, Jiang Hu, Xiu Liu, Shaobin Li, Mingna Li, Bingang Shi, Liyan Hu, Yuan Liu, and et al. 2022. "MicroRNA-200c Affects Milk Fat Synthesis by Targeting PANK3 in Ovine Mammary Epithelial Cells" International Journal of Molecular Sciences 23, no. 24: 15601. https://doi.org/10.3390/ijms232415601