m6A-Mediated PPARA Translational Suppression Contributes to Corticosterone-Induced Visceral Fat Deposition in Chickens
Abstract
:1. Introduction
2. Results
2.1. Chronic CORT Exposure Induces Visceral Fat Deposition
2.2. Chronic CORT Exposure Decreases Lipolytic Functions in Visceral Fat Tissue
2.3. Chronic CORT Exposure Inhibits Global m6A Methylation Levels in Visceral Fat Tissue
2.4. Chronic CORT Exposure Decreased Site-Specific RNA Methylation in Exon7 of PPARA
2.5. METTL3 Knockdown Decreased PPARA Protein Content via m6A Demethylation in CDS Region
3. Discussion
4. Materials and Methods
4.1. Animals and Treatment
4.2. HE Staining
4.3. Plasma Parameters Determination
4.4. RNA Isolation and Real-Time PCR
4.5. Western Blot
4.6. Global and Site-Specific M6A Detection
4.7. Plasmids Construction
4.8. Cell Culture and Transfection
4.9. Immunofluorescence Analysis
4.10. Deadenylation and Decay Assays
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
Appendix A
Target Genes | Primer Sequences (5′ to 3′) | PCR Products (bp) | Amplification Efficiency (%) |
---|---|---|---|
GR | TGCACAAGCCCCTGTATCTG | 265 | 102.21% |
NM_001037826.1 | AATGCTTTCCTCCAGGAGCC | ||
HSD11B1 | TTGGGACTGGTTTTGGCCTT | 238 | 98.84% |
XM_025143927.3 | TGGCCTTCCTGTGTTTGTGT | ||
HSD11B2 | AGAGAGTGATCGTGACGGGA | 294 | 107.23% |
XM_417988.8 | CGTCCCCGTTGAAGAAGTCA | ||
ABCD2 | CACACAGACCTTCTCTTTGTACC | 136 | 93.43% |
NM_001199395.2 | CCTGCAAGTTGGGACTCCAG | ||
ACAA1 | TGGAAAACAGCAAAGCTCGC | 117 | 91.29% |
NM_001197288.2 | GTTGGGAAGCCAAGGCAAAG | ||
ACAA2 | TAATGGGCATTGGCCCTGTT | 280 | 91.29% |
NM_001006571.3 | CCAATGCAAGCTGACCCAAC | ||
ATG7 | CTGAGGGGCATGGAGGATGT | 100 | 93.43% |
NM_001030592.2 | CCCAACTCCCCTTCTGGAAC | ||
TFAM | AAAGCTTCGCTGCGTGAAAG | 242 | 92.71% |
NM_204100.2 | CACAGCTCAGGTTACACCGT | ||
ATP5A | TCTGGCTCCTTACTCAGGCT | 143 | 101.78% |
NM_001001935.3 | AAAGCAGTCAGAGAGCCACC | ||
VDAC | TGCTGATCTGGGCAAGTCTG | 201 | 103.54% |
NM_001033869.3 | TTCTCCGTGAACATCAGCCC | ||
PPARGC1A | AAAACGGAGGGATGGCGAAT | 134 | 98.03% |
NM_001006457.2 | TCGAGCAGCTGTGTTCATGT | ||
CA4 | GTGAAGACCCTCGACACTGG | 282 | 100.92% |
XM_415893.8 | GCTGTTCCTCTTGGACTCCC | ||
CIDEA | ATGGTTGCAAGCAAAGCTGG | 103 | 104.89% |
NM_001195123.2 | GTGATGCAAAGCAACAGGCA | ||
CD137 | TTGCCTTGCTGCTCTACCTC | 117 | 93.80% |
NM_001025366.3 | AGCTGCGCTGATAGACATCC | ||
TMEM26 | TGATGGTTCTGCTCAACGCT | 145 | 91.29% |
NM_001199598.2 | CCTGTCTCCAGGCAAAGGAG | ||
RB1 | GAAACCTCCACAAACGGCAC | 184 | 99.25% |
NM_204419.2 | GCCCCAATGCAACACACATT | ||
RBL1 | CACAAAGTGTGAGCCGGTTG | 285 | 94.17% |
XM_417312.8 | TCCCATGCAGTCTTCGCATT | ||
HSL | TATGGGAGGTGTCTCGGGTT | 231 | 109.34% |
XM_040657096.1 | AAGCTCTTCCAGAAGCCCAC | ||
ATGL | TCACCTTCAGCGTCCAAGTC | 267 | 108.68% |
NM_001113291.1 | AGCAGGTAGGGGACCATCAT | ||
PLIN1 | GACCACAGCAAGGTACACGA | 177 | 102.56% |
XM_015292005.3 | GATTGCTGCTGGGAGACCTT | ||
PPARA | ATGCCAAGGTCTGAGAAGGC | 168 | 108.68% |
XM_025150258.2 | CCCTGCAAGGATGACTCTGG | ||
PPARG | ACCTCACGAGGAGTCTTCCA | 287 | 94.92% |
NM_001001460.1 | GCTTCTCCTTCTCCGCTTGT | ||
CEBPB | CTCCTACCTGGGCTACCAGT | 195 | 95.30% |
NM_205253.2 | TTGTACTCGTCGCTGTGCTT | ||
FASN | GCTAAGATGGCATTGCACGG | 300 | 106.28% |
NM_205155.3 | TGCCAGAGCCTCCACTATCT | ||
SCD1 | ACACCTTAGGGCTCAATGCC | 171 | 98.84% |
NM_204890.1 | TGGTGGAGTAGTCGTAGGGG | ||
CD36 | CTCTTTGTGGCCTTTGCACC | 291 | 107.71% |
XM_040686380.1 | CAGCACAGCACCAATGACAG | ||
FATP | GGTGAGAAGTGGACCTTCCG | 165 | 102.21% |
NM_001039602.2 | CGCTTCGATGCTGACTTTCG | ||
Beclin1 | ACAGTGGTCAGTTTGGCACA | 285 | 105.82% |
NM_001006332.1 | AGAAAGCCACCATCGCATGA | ||
LC3 | GTTTCCAATGCCTGCGTGTT | 244 | 94.54% |
NM_001031461.1 | AACAGGGTCCTAATGCCAGC | ||
FTO | TCACCAAGGCGACCTCTACT | 88 | 103.54% |
XM_040707054.1 | GCTGAACCGAGGTGAAAAGC | ||
METTL14 | ATTCGACCAGGATGGCTGAC | 155 | 99.25% |
XM_040698977.1 | GACTTGGGTGGTGGTGACTT | ||
METTL3 | ATCCTGGAGCTGCTCAACAC | 195 | 108.20% |
XM_040655036.1 | AGATTCGTCCGTGTGCTTGT | ||
YTHDF1 | AGTTCAGCATACGGGAGCAG | 236 | 93.07% |
XM_040650685.1 | CCACCATTACCAGAGAGGGC | ||
YTHDF2 | AAGGCCAAGGCAACAAAGTG | 110 | 94.54% |
XM_040690125.1 | ATATGCATTGTTCGGCCGGG | ||
YTHDF3 | GTGACCCTCCAATGCCATACT | 108 | 105.82% |
NM_001006391.1 | GGGTGTTTCCTAATGCCCCA | ||
PPIA | GTCGTGTTCTTCGACATCGC | 265 | 106.76% |
XM_046903390.1 | CGAAGAGAAAATGGCGGCG |
Name | X Site | N Site |
---|---|---|
ATGL-3’UTR-up | tagccagtaccgtagtgcgtgGCAGGCACTCTTTTGCACAG | tagccagtaccgtagtgcgtgTCTTTTGCACAGTCCAGAGG |
ATGL-3’UTR-down | CCAGAGGTGGAAGTTTCATCcagaggctgagtcgctgcat | GGAAGTTTCATCTGAAATCCcagaggctgagtcgctgcat |
PPARA-Exon7-up | tagccagtaccgtagtgcgtgTTCGGTAAGTTCTGTGACAG | tagccagtaccgtagtgcgtgTGTGACAGTCTCTACAGAGG |
PPARA-Exon7-down | CTCTACAGAGGTACACTGGCcagaggctgagtcgctgcat | ACACTGGCAGCAGTGGAAGAcagaggctgagtcgctgcat |
PPARA-Lastexon-up | tagccagtaccgtagtgcgtgTTAAAAATCCTTAATACATG | tagccagtaccgtagtgcgtgTGTGACAGTCTCTACAGAGG |
PPARA-Lastexon-down | CCCTGTAGATTTCCTGCAGTcagaggctgagtcgctgcat | ACACTGGCAGCAGTGGAAGAcagaggctgagtcgctgcat |
BECN1-Exon1-up | tagccagtaccgtagtgcgtgCATGAATTTTAGTGCTTTTG | tagccagtaccgtagtgcgtgTTAGTGCTTTTGTCCATTGC |
BECN1-Exon1-down | CCATTGCTCCTCAGAGTTAAcagaggctgagtcgctgcat | CCTCAGAGTTAAATTGTGTTcagaggctgagtcgctgcat |
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Zhou, Z.; Zhang, A.; Liu, X.; Yang, Y.; Zhao, R.; Jia, Y. m6A-Mediated PPARA Translational Suppression Contributes to Corticosterone-Induced Visceral Fat Deposition in Chickens. Int. J. Mol. Sci. 2022, 23, 15761. https://doi.org/10.3390/ijms232415761
Zhou Z, Zhang A, Liu X, Yang Y, Zhao R, Jia Y. m6A-Mediated PPARA Translational Suppression Contributes to Corticosterone-Induced Visceral Fat Deposition in Chickens. International Journal of Molecular Sciences. 2022; 23(24):15761. https://doi.org/10.3390/ijms232415761
Chicago/Turabian StyleZhou, Zixuan, Aijia Zhang, Xinyi Liu, Yang Yang, Ruqian Zhao, and Yimin Jia. 2022. "m6A-Mediated PPARA Translational Suppression Contributes to Corticosterone-Induced Visceral Fat Deposition in Chickens" International Journal of Molecular Sciences 23, no. 24: 15761. https://doi.org/10.3390/ijms232415761