Yeast Protein Asf1 Possesses Modulating Activity towards Protein Kinase CK2
Abstract
:1. Introduction
2. Results
3. Materials and Methods
3.1. Materials
3.2. Purification of Yeast CK2
3.3. Overexpression and Purification of Human CK2α and CK2α’ Subunits
3.4. Purification of Human CK2 Holoenzymes
3.5. Cloning of the Asf1, Asf11-169, and Asf1170-279 Proteins into Bacterial Expression Vector pET28a
3.6. Cloning of Asf1 Protein and the C-Terminal Fragment Asf1170-279 into Yeast Expression Vector pYES2/CT
3.7. Overexpression and Purification of Asf1, Asf11-169, and Asf1170-279 Proteins
3.8. Protein Substrates
3.9. Protein Phosphorylation
3.10. Asf1 Digestion by Trypsin
3.11. In Vivo Analysis
3.12. Western Blot Analysis
3.13. Statistical Analysis and Management Data
3.14. CABS-Dock Method
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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CK2α | Asf1 170-193 | Distance (Å) | CK2α’ | Asf1 170-193 | Distance (Å) |
---|---|---|---|---|---|
K158 | D177 | 3.553 | K50 | E174 | 3.291 |
K158 | E180 | 3.295 | K50 | E175 | 3.061 |
E180 | D189 | 2.938 | K50 | E178 | 3.141 |
Q186 | E187 | 3.302 | S195 | E180 | 2.826 |
E230 | D170 | 2.747 | S195 | E180 | 2.845 |
R196 | E180 | 3.362 | |||
E231 | D186 | 3.084 | |||
G236 | D181 | 2.983 | |||
K280 | D192 | 3.249 |
Protein | Expression Vector | Primer Sequences * |
---|---|---|
Asf1 | pET28a | 5′-CCGGATCCTCAATTGTTTCACTGTTAGGCA-3′ 5′-ACGTCGACTTAATTCGTTGAACGTGCCGCA-3′ |
Asf11-169 | pET28a | 5′-CCGGATCCTCAATTGTTTCACTGTTAGGCA-3′ 5′-CGGTCGACTTATAC GCCGGGCTGTTCAG-3′ |
Asf1170-279 | pET28a | 5′-ACGGATCCGATGATGAAGAGGAGGAGGACG-3′ 5′-ACGTCGACTTAATTCGTTGAACGTGCCGCA-3′ |
Asf1 | pYES2/CT | 5′-CCAAGCTTGGAAAAATGTCAATTGTTTCACTG-3′ 5′-CCGAATTCTTAATTCGTTGAACGTGCCGC-3′ |
Asf1170-279 | pYES2/CT | 5′-CCAAGCTTGGAAAAATGGATGATGAAGAGGAG-3′ 5′-CCGAATTCTTAATTCGTTGAACGTGCCGC-3′ |
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Baier, A.; Szyszka, R.; Jach, M.E. Yeast Protein Asf1 Possesses Modulating Activity towards Protein Kinase CK2. Int. J. Mol. Sci. 2022, 23, 15764. https://doi.org/10.3390/ijms232415764
Baier A, Szyszka R, Jach ME. Yeast Protein Asf1 Possesses Modulating Activity towards Protein Kinase CK2. International Journal of Molecular Sciences. 2022; 23(24):15764. https://doi.org/10.3390/ijms232415764
Chicago/Turabian StyleBaier, Andrea, Ryszard Szyszka, and Monika Elżbieta Jach. 2022. "Yeast Protein Asf1 Possesses Modulating Activity towards Protein Kinase CK2" International Journal of Molecular Sciences 23, no. 24: 15764. https://doi.org/10.3390/ijms232415764