Gonadal Transcriptome Analysis and Sequence Characterization of Sex-Related Genes in Cranoglanis bouderius
Abstract
:1. Introduction
2. Results
2.1. Sequencing Quality Assessment
2.2. Transcriptome Data Validation
2.3. Analysis of Differentially Expressed Genes (DEGs)
2.4. Identification of Sex-Related DEGs
2.5. Sequence Characterization of Sex-Related Genes
2.6. Sex-Related Gene Tissue Expression
3. Discussion
4. Materials and Methods
4.1. Experimental Fish and Sample Collection
4.2. RNA Extraction, Library Building and Sequencing
4.3. Transcriptome Data Validation
4.4. Identification and Functional Annotation of Differentially Expressed Genes (DEGs)
4.5. Sequence Characterization of Sex-Related Gene
4.6. Sex-Related Gene Tissue Expression Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | Male 1 | Male 2 | Male 3 | Female 1 | Female 2 | Female 3 |
---|---|---|---|---|---|---|
Raw reads | 50,755,254 | 44,882,700 | 54,264,414 | 51,980,490 | 59,555,972 | 56,712,898 |
Raw bases (GB) | 7.66 | 6.77 | 8.19 | 7.84 | 8.99 | 8.56 |
Clean reads | 50,230,124 | 44,382,438 | 53,706,854 | 51,603,750 | 59,081,800 | 56,248,822 |
Clean bases (GB) | 7.38 | 6.50 | 7.89 | 7.64 | 8.76 | 8.34 |
Error rate (%) | 0.0246 | 0.0246 | 0.0247 | 0.0241 | 0.0241 | 0.0244 |
Q20 (%) | 98.11 | 98.07 | 98.05 | 98.34 | 98.34 | 98.21 |
Q30 (%) | 94.64 | 94.63 | 94.56 | 95.11 | 95.11 | 94.77 |
GC (%) | 49.04 | 49.1 | 49.98 | 50.19 | 50.26 | 50.45 |
Mapped | 46,878,397 | 41,483,671 | 50,154,719 | 49,439,775 | 56,398,939 | 53,722,386 |
Mapped (%) | 93.33 | 93.47 | 93.39 | 95.81 | 95.46 | 95.51 |
Uniquely mapped | 43,477,040 | 38,131,053 | 45,239,980 | 44,625,697 | 51,147,482 | 48,902,184 |
Uniquely mapped (%) | 86.56 | 85.91 | 84.24 | 86.48 | 86.57 | 86.94 |
Clean reads | 50,230,124 | 44,382,438 | 53,706,854 | 51,603,750 | 59,081,800 | 56,248,822 |
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Wang, D.; Pan, Z.; Wang, G.; Ye, B.; Wang, Q.; Zuo, Z.; Zou, J.; Xie, S. Gonadal Transcriptome Analysis and Sequence Characterization of Sex-Related Genes in Cranoglanis bouderius. Int. J. Mol. Sci. 2022, 23, 15840. https://doi.org/10.3390/ijms232415840
Wang D, Pan Z, Wang G, Ye B, Wang Q, Zuo Z, Zou J, Xie S. Gonadal Transcriptome Analysis and Sequence Characterization of Sex-Related Genes in Cranoglanis bouderius. International Journal of Molecular Sciences. 2022; 23(24):15840. https://doi.org/10.3390/ijms232415840
Chicago/Turabian StyleWang, Dongjie, Zhengkun Pan, Guoxia Wang, Bin Ye, Qiujie Wang, Zhiheng Zuo, Jixing Zou, and Shaolin Xie. 2022. "Gonadal Transcriptome Analysis and Sequence Characterization of Sex-Related Genes in Cranoglanis bouderius" International Journal of Molecular Sciences 23, no. 24: 15840. https://doi.org/10.3390/ijms232415840