Remarkable Plasticity of Bone Iron Homeostasis in Hibernating Daurian Ground Squirrels (Spermophilus dauricus) May Be Involved in Bone Maintenance
Abstract
:1. Introduction
2. Results
2.1. Bone Mass, Mechanical Properties, and Microstructure
2.2. Total Iron Content in Femur and Liver during Different Periods
2.3. Iron Distribution in Femur and Liver during Different Periods
2.4. Regulation of Hepcidin in Liver during Different Periods
2.5. Iron-Metabolism-Related Protein Expression in Femur during Different Periods
3. Discussion
4. Materials and Methods
4.1. Animals and Groups
4.2. Sample Collection and Preparation
4.3. Micro-Computed Tomography (CT)
4.4. Mechanical Properties
4.5. Iron Measurements
4.6. Histological Analysis
4.7. Enzyme-Linked Immunosorbent Assay (ELISA)
4.8. Western Blot Analysis
4.9. Statistical Analysis
5. Conclusions
6. Limitations
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Group | Sample Time | Tb of Animal |
---|---|---|
SA | Mid-June | >37 °C |
PRE | Mid-September | >37 °C |
TOR | After two months hibernation, animals enter a new hibernation bout | ≥5 days with stable Tb of 5–8 °C |
IBA | Same as TOR | 34–37 °C for less than 12 h |
NP | March of following year | 5–8 °C for less than 24 h |
POST | Same as NP | 36–38 °C for more than 3 d |
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He, Y.; Kong, Y.; Yin, R.; Yang, H.; Zhang, J.; Wang, H.; Gao, Y. Remarkable Plasticity of Bone Iron Homeostasis in Hibernating Daurian Ground Squirrels (Spermophilus dauricus) May Be Involved in Bone Maintenance. Int. J. Mol. Sci. 2022, 23, 15858. https://doi.org/10.3390/ijms232415858
He Y, Kong Y, Yin R, Yang H, Zhang J, Wang H, Gao Y. Remarkable Plasticity of Bone Iron Homeostasis in Hibernating Daurian Ground Squirrels (Spermophilus dauricus) May Be Involved in Bone Maintenance. International Journal of Molecular Sciences. 2022; 23(24):15858. https://doi.org/10.3390/ijms232415858
Chicago/Turabian StyleHe, Yue, Yong Kong, Rongrong Yin, Huajian Yang, Jie Zhang, Huiping Wang, and Yunfang Gao. 2022. "Remarkable Plasticity of Bone Iron Homeostasis in Hibernating Daurian Ground Squirrels (Spermophilus dauricus) May Be Involved in Bone Maintenance" International Journal of Molecular Sciences 23, no. 24: 15858. https://doi.org/10.3390/ijms232415858